[1]杨喆,刘丽君,高明杰,等.大豆高油相关QTL分子标记辅助选择研究[J].大豆科学,2008,27(06):921-924.[doi:10.11861/j.issn.1000-9841.2008.06.0921]
　YANG Zhe,LIU Li-jun,GAO Ming-jie,et al.Molecular Marker Assistant Selection for High Oil QTL in Soybean[J].Soybean Science,2008,27(06):921-924.[doi:10.11861/j.issn.1000-9841.2008.06.0921]
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大豆高油相关QTL分子标记辅助选择研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年06期 页码: 921-924 栏目: 出版日期: 2008-12-25
Title:
Molecular Marker Assistant Selection for High Oil QTL in Soybean
文章编号:
1000-9841(2008)06-0921-04
作者:
杨喆; 刘丽君; 高明杰; 蒲国锋; 张雷
黑龙江省农业科学院大豆研究所，黑龙江 哈尔滨 150086
Author(s):
YANG Zhe; LIU Li-jun; GAO Ming-jie; PU Guo-feng; ZHANG Lei
Soybean Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang，China
关键词:
大豆;  SSR; 高油QTL; 分子标记辅助选择
Keywords:
Soybean; SSR; High oil QTL ;  Marker assistant selection
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.06.0921
文献标志码:
A
摘要:
选用高产大豆品系哈交5448-4和高油大豆品种黑农45为亲本杂交获得F2分离群体，进行大豆高油基因SSR分子标记。共筛选覆盖大豆全基因组的325对SSR引物，利用筛选出的63对在亲本间具有多态性的SSR引物对F2分离群体的120个单株进行SSR扩增，经电泳检测后，所得数据用于作图和定位分析。定位到高油QTL 1个，与satt160连锁，遗传距离为26.0cM，贡献率为23.20％，位于大豆公共遗传图谱的F连锁群。利用该引物在24份大豆材料中进行高油材料检测和筛选，在15份高油材料中筛选到11份，检出率达到73.33％。结果说明satt160具有一定的检测通用性，可以利用它对高油大豆材料进行分子标记辅助选择。
Abstract:
Soybean seeds contain high level of oil which is useful for human consumption.Increasing emphasis ?in breeding programs to produce soybean with specific oil content for specialty markets demands that more efficient manipulation of these traits have be achieved.Heinong 45 and Hajiao5448-4 were crossed to obtain the F2 separate population in this research，and the 120 F2：3individuals were used to tag SSR molecular marker for high oil gene.Three hundred and twenty-five pair of SSR primers covering the whole soybean genome were screened，and 63 pair of primers have the polymorphism.Composite Interval Mapping method (CIM) were used and one high oil QTL linking with satt160 was located on LG F，the genetic distance was 26.0 cM and the variance ratio was 23.20%.Satt160 was used to exam and screen the high oil content soybean materials，eleven share were screened from 15 high oil content soybean materials，the ratio of the examination was 73.33%.Result suggests that satt160 can be used to screen the high oil content soybean materials for marker assistant selection.

参考文献/References:

[1]方宣均，吴为人，唐纪良.作物DNA标记辅助育种[M].北京：科学出版社，2001.（Fang X J，Wu W R，Tang J L.Crops DNA marker assistant breeding[M].Beijing:Science Press，2001.）

[2] Mansur L M，Orf J，Lark K G.Determining the linkage of quantitative trait loci to RFLP maker using extreme phenotype of recombinant inbreds of soybean(Glycine max L.Merr.)[J].Theoretical and Applied Genetics，1993，86:914-918.
[3]吴晓雷，王永军，贺超英，等.大豆重要农艺性状的QTL分析[J].遗传学报，2001， 28(10):947-955.（Wu X L，Wang Y J，He C Y，et al.QTLs mapping of some agronomic traits of soybean[J].Acta Genetica Sinica，2001，28(10):947-955.）
[4]Akkaya M S，Bhagwat A A，Cregan P B.Length polymorphism of simple sequence repeat DNA in soybean[J].Genetics，1992，132:1131-1139.
[5]Akkaya M S，Shoemaker R C，Specht J E，et al.Integration of simple sequence repeat DNA markers into a soybean linkage map[J].Crop Science，1995，35:1439-1445.
[6]Cregan P B，Jarvik T.An integrated genetic linkage map of the soybean genome[J].Crop Science，1999，35(5):1464-1490.
[7] Shoemaker R C，Specht J E.Integration of the soybean molecular and classical genetic linkage groups[J].Crop Science，1995，35:436-446.
[8]关荣霞，常汝镇，邱丽娟.用于SSR分析的大豆DNA的快速提取[J].大豆科学，2003，21(1):73-74.（Guan R X，Chang R Z，Qiu L J.Rapid isolation of soybean DNA for SSR analysis[J].Soybean Science，2003，21(1):73-74.)
[9]宛煜嵩.大豆遗传图谱的构建及若干农艺性状的QTL定位分析[D].北京：中国农业科学院，2002.（Wan Y S.Construction of soybean genetic map and QTL analysis of some agronomic traits[D].Beijing: Chinese Academy of Agricultural Sciences，2002.
[10]Schneider K A，Brothers M E，Kelly J D.Marker assisted selection to improve drought resistance in common bean[J].Crop Science，1997，37:51-60.
[11]刘峰，庄炳昌，张劲松，等.大豆遗传图谱得构建和分析[J].遗传学报，2000，27(11): 1018-1026.（Liu F，Zhuang B C，Zhang J S，et al.Construction and analysis of soybean genetic map[J].Acta Genetica Sinica，2000，27(11):1018-1026.）
[12]蒙忻，刘学义，方宣钧.利用大豆分子连锁图定位大豆孢囊线虫4号生理小种抗性QTL[J].分子植物育种，2003，1(1):6-21.（Meng X，Liu X Y，Fang X J.QTL Mapping genes conferring resistance to race 4 of soybean cyst nematode in soybean ZDD2315 (Glycine max(L) Merr.) based on public molecular genetic linkage map[J].Molecular Plant Breeding，2003，1(1):6-21.）
[13]许占友，常汝镇，邱丽娟，等.大豆遗传图谱研究进展及对应的几个问题[J].大豆科学，2001，20(2):133-137.（Xu Z Y，Chang R Z，Qiu L J，et al.The research evolvement for soybean genetic map and its some problems[J].Soybean Science，2001，20(2):133-137.）
[14]张德水，董伟，惠东威，等.用栽培大豆与野生大豆间的杂种F2群体构建基因组分子标记连锁图谱框架图[J].科学通报，1997，42(2):1326-1330.（Zhang D S，Dong W，Hui D W，et al.Construction of a soybean linkage map using an F2 hybrid population from a cultivated variety and a semi wild soybean[J].Chinese Science Bulletin,1997，42(2):1326-1330.）
[15]Brummer E C，Graef G L，Orf J，et al.Mapping QTL for seed protein and oil content in eight soybean populations[J].Crop Science，1997，37:370-378.
[16] Csanádi G，Vollmann J，Stift G，Lelley T.Seed quality QTLs identified in a molecular map of early maturing soybean[J].Theoretical and Applied Genetics，2001，103:912-919.
[17]Lander E S，Green P，Abrahamson J，et al.Mapmaker:an interactive computer package for constructing genetic linkage maps of experimental and natural populations[J].Genomics，1987，1:174-181.
[18]Li Z，Jakkula L，Hussey R S，et al.SSR mapping and confirmation of the QTL from PI96354 conditioning soybean resistance to southern root-knot nematode[J].Theoretical and Applied Genetics，2001，103:1167-1173.

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备注/Memo

基金项目：黑龙江省自然科学基金资助项目（ZTN04-0401）；引进国际先进农业科学技术计划（948计划）资助项目（2006-G5）；国家高技术研究发展计划（863计划）资助项目（2006AA10Z1F9）。

作者简介：杨喆（1978-），男，助理研究员,硕士，主要从事大豆分子标记及大豆遗传育种研究。E-mail：yz78100@163.com。

更新日期/Last Update: 2014-10-04