NIE Teng-kun,ZHAO Lin,LI Hai-yan,et al.Cloning and Bioinformatics Analysis of MPBQ-MT Gene in Soybean[J].Soybean Science,2016,35(01):39-45.[doi:10.11861/j.issn.1000-9841.2016.01.0039]
大豆-MPBQ-MT-基因的克隆与生物信息学分析
- Title:
- Cloning and Bioinformatics Analysis of MPBQ-MT Gene in Soybean
- 关键词:
- 大豆; 维生素E; MPBQ-MT-基因; 生物信息学分析
- Keywords:
- Soybean; Vitamin E; MPBQ-MT gene; Bioinformatics analysis
- 文献标志码:
- A
- 摘要:
- 以大豆品种合丰25和Bayfield为材料,克隆大豆2-甲基-6-植基-1,4-苯醌甲基转移酶(MPBQMTase)基因,获得1 029 bp的MPBQ-MT-基因cDNA序列。通过序列比对发现10个大豆MPBQ-MT-基因cDNA区内的潜在SNP位点。采用生物信息学方法分析MPBQ-MT-基因编码的氨基酸序列和蛋白质结构。结果表明:MPBQ-MT-基因CDS区共编码342个氨基酸,其中缬氨酸(Val)含量最多,占该基因编码氨基酸总数的8.8%;半胱氨酸(Cys)含量最少,占该基因编码氨基酸总数的1.2%;MPBQ-MT蛋白N端包含由58个氨基酸组成的叶绿体转运肽,为亲水性蛋白质。合丰25与Bayfield的MPBQ-MT蛋白质二级结构有所不同,2个品种的MPBQ-MT蛋白质中α-螺旋分别约占25.44%和27.49%,β-转角分别约占9.36%和9.06%,无规则卷曲分别约占40.35%和38.60%。2个品种的MPBQ-MT蛋白质中片层结构均约占24.85%。两个品种的MPBQ-MT蛋白质均有1个S-腺苷基甲硫氨酸结合位点。对MPBQ-MT蛋白三级结构进行预测,发现去掉转运肽后,MPBQ-MT蛋白质三级结构主要由11个连续的α-螺旋区域和8个连续的片层结构区域组成。结合MPBQ-MT蛋白三级结构对MPBQ-MT-基因的生物学功能进一步分析,提出了较为合理的MPBQ-MT作用模型。由系统发生树可知MPBQ-MT蛋白在大豆与菜豆中的亲缘关系较近。
- Abstract:
- Cloned the 2-methyl-6-phytyl-1,4-benzoquinone methyltransferase (MPBQMTase) of soybean and obtained the cDNA encoding MPBQMTase using Hefeng 25 and Bayfield as materials. The cDNA sequence analysis showed that the cDNA was 1 029 bp in length.Ten latent SNPs were found in CDS of MPBQ-MT gene in soybean by sequences blast.The amino acid sequence and protein structure of MPBQ-MT gene were analyzed by bioinformatics method. The result showed that the CDS region of MPBQ-MT gene encoded 342 amino acid residues with the most content of Valine which accounted for 8.8% of the total amino acids and the least content of Cysteine which accounted for 1.2% of the total amino acids N-terminal of MPBQ-MT protein contains chloroplast transit peptide composed by 58 terminal amino acids and MPBQ-MT was hydrophilic protein. The secondary structure of MPBQ-MT protein was different in Hefeng 25 and Bayfield.About 25.44% and 27.49% of the protein sequence were alpha helixes respectively in the two varieties, about 9.36% and 9.06% of the protein sequence were beta turn, 40.35% and 38.60% of the protein sequence were random coil 24.85% of the protein sequence were lamellar structure in both varieties. S-adenosyl methionine binding sites exist in MPBQ-MT protein of both varieties.The three stage structure analysis showed 11 continuous alpha helixes and 8 continuous film layer structure exist in MPBQ-MT protein. The model of MPBQ-MT was obtained based on the three stage structure of MPBQ-MT protein and biological function analysis of MPBQ-MT. Phylogenetic tree indicated the relationship of MPBQ-MT protein was close in soybean and bean.
参考文献/References:
[1]Sen C K, Khanna S, Roy S. Tocotrienols: Vitamin E beyond tocopherols [J]. Life Sciences, 2006, 78(18):2088-2098.[2]Fryer M J. The antioxidant effects of thylakoid Vitamin E(α-tocopherol) [J]. Plant, Cell & Environment, 1992, 15(4): 381-392.
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备注/Memo
基金项目:中国博士后项目(20110491025);东北农业大学博士启动基金(2012RCB32)。