[1]张郁松.影响大豆愈伤组织的诱导因素研究[J].大豆科学,2007,26(01):66-70.[doi:10.3969/j.issn.1000-9841.2007.01.015]
　ZHANG Yu-song.RESEARCH ON AFFECTIVE FACTOR OF SOYBEAN CALLUS INDUCTION[J].Soybean Science,2007,26(01):66-70.[doi:10.3969/j.issn.1000-9841.2007.01.015]
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影响大豆愈伤组织的诱导因素研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年01期 页码: 66-70 栏目: 出版日期: 2007-02-25
Title:
RESEARCH ON AFFECTIVE FACTOR OF SOYBEAN CALLUS INDUCTION
文章编号:
1000-9841(2007)01-0066-05
作者:
张郁松
西安武警工程学院军事经济系，西安710086
Author(s):
ZHANG Yu-song
Xian Engineering College of Armed Police Force，Xi-an，710086
关键词:
大豆; 愈伤组织; 诱导
Keywords:
Soybean;  Callus;  Induction
分类号:
S565.103.53
DOI:
10.3969/j.issn.1000-9841.2007.01.015
文献标志码:
A
摘要:
比较MS、B5、N6三种基本培养基，发现MS适合作为诱导大豆愈伤组织的基本培养基。添加激素6-BA 0.5mg/L， 2，4-D 2.0mg/L时愈伤组织诱导的效果最好，过高或过低的浓度激素均不利于愈伤组织的诱导和生长；不同外植体诱导愈伤组织的能力不一样，下胚轴诱导愈伤组织能力强于子叶；在所选择的六种大豆品种中，中黄13诱导愈伤组织的能力最强；光照条件为12h/d时有利于愈伤组织的诱导；培养基pH5.8～6.0时，愈伤组织的诱导率比较高。
Abstract:
Among the 3 media tested， MS was the most suitable for the callus induction of soybean. The medium added hormone with 0.5mg/L 6-BA and 2.0 mg/L 2，4-D was the best one for the induction of callus. Neither higher nor lower would suitable for the induction of callus. The effects of explants type on soybean callus induction were different， and the hypocotyls was more effecive than cotyledon on soybean callus induction. Among the 6 soybean types， zhonghuang13 was the most suitable for the callus induction of soybean. 12 hour light and 12 hour dark were benefit for the callus induction. The best pH for the callus induction and subculture was between 5.8 and 6.0.

参考文献/References:

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备注/Memo

作者简介：张郁松（1974-），男，讲师，硕士，研究方向生物制药。E-mail:zhangyusong123@126.com

更新日期/Last Update: 2014-10-21