[1]李文霞,李文滨,吕文河,等.农杆菌介导大豆子叶节系统的两个问题突破[J].大豆科学,2008,27(01):173-175，180.[doi:10.11861/j.issn.1000-9841.2008.01.0173]
　LI Wen-xia,LI Wen-bin,Lv Wen-he,et al.Breakthrough of Two Questions on the Agrobacterium-mediated Soybean Cotyledonary Node Systems[J].Soybean Science,2008,27(01):173-175，180.[doi:10.11861/j.issn.1000-9841.2008.01.0173]
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农杆菌介导大豆子叶节系统的两个问题突破

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年01期 页码: 173-175，180 栏目: 出版日期: 2008-02-25
Title:
Breakthrough of Two Questions on the Agrobacterium-mediated Soybean Cotyledonary Node Systems
作者:
李文霞1; 李文滨2; 吕文河1; 宁海龙2
1东北农业大学农学院，黑龙江哈尔滨 150030；
2东北农业大学大豆研究所，教育部大豆生物学重点试验室，黑龙江哈尔滨 150030
Author(s):
LI Wen-xia1; LI Wen-bin2; Lv Wen-he1; and NING Hai-long2
1College of Agronomy，Northeast Agricultural University，Harbin 150030，Heilongjiang;
2Soybean Research Institute of Northeast Agricultural University，Key Laboratory of Soybean Biology in Chinese Ministry of Education，Harbin 150030，Heilongjiang，China
关键词:
大豆; 子叶节; 丛生芽伸长; 愈伤组织
Keywords:
Soybean; Cotyledonary node; Multiple shoots elongation; Calli tissue
DOI:
10.11861/j.issn.1000-9841.2008.01.0173
文献标志码:
A
摘要:
为解决大豆子叶节系统存在的再生和检测问题，对影响丛生芽伸长和植株检测的因素进行研究。以大豆品种黑农35的子叶节为外植体，利用L934正交试验确定影响再生系统丛生芽伸长的因素及水平，将转基因小植株的叶片培养愈伤组织以提取基因组DNA进行Southern检测。结果表明，丛生芽伸长的最佳组合为继代培养基与初始芽诱导培养基的6-BA浓度比为1/5，芽伸长培养基中添加50 mg L-1Glu、0.5 mg L-1GA3、0.5 mg L-1ZT；Southern杂交信号说明利用叶片培养的愈伤组织能够提取足够量的基因组DNA。
Abstract:
The factors influencing soybean（Glycine max L.） cotyledonary node regeneration and detection from soybean cultivars “Heinong 35” were studied in this research so as to solve the two problems.L934orthogonal design was used to discuss effect of four key factors and levels on multiple shoot elongation.DNA for Southern blot were extracted from calli tissue which were induced from putative transgenic plants leaves.The results indicated that the better conditions for elongation were got when concentration of 6-Benzylaminopurine in subculture medium for shoot induction was dropped to 1/5 to the preceding shoot induction medium，and 50 mg L-1Glutamic acid，0.5 mg L-1Gibberellin A3 and 0.5 mg L-1Zeatin were added to shoot elongation medium；Southern blot signal indicated that calli tissue from leaves could extract enough genomic DNA.

参考文献/References:

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备注/Memo

基金项目：黑龙江省科技攻关项目“大豆、马铃薯抗病和抗虫基因工程的研究”（GB04B101）

作者简介：李文霞（1974-），女，讲师，博士，研究方向为植物分子生物学。Tel:13796674549；E-mail:wxlee2006@yahoo.com.cn
通讯作者 (Corresponding author)：李文滨，教授，博士。Tel: 55190778； E-mail:wenbinli@yahoo.com；吕文河，教授，博士。Tel: 55191763； E-mail:whlu@mail.neau.edu.cn；宁海龙，副教授，博士。Tel: 55191042； E-mail:ninghailong1975@163.com

更新日期/Last Update: 2014-10-10