ZHANG Yu-song,KOU Wei-cai.RESEARCH ON THE OPTIMUM CONDITIONS FOR SOYBEAN CALLUS SUBCULTURE[J].Soybean Science,2007,26(02):190-193.[doi:10.3969/j.issn.1000-9841.2007.02.015]
大豆愈伤组织继代培养的最佳条件研究
- Title:
- RESEARCH ON THE OPTIMUM CONDITIONS FOR SOYBEAN CALLUS SUBCULTURE
- 文章编号:
- 1000-9841(2007)02-0190-04
- Keywords:
- Soybean; Callus; Subculture
- 分类号:
- S565.103.53
- 文献标志码:
- A
- 摘要:
- 探讨基本培养基、培养条件、植物生长调节剂等因素对大豆继代培养的影响。大豆愈伤组织继代培养的最佳条件:以MS+BA0.5 mg/L+2,4-D2.0 mg/L为培养基,pH在5.8~6.0之间,在1 000~1 500Lx,2 h/d光照条件下,培养21 d后生长较好,愈伤组织生长量和增长倍数可分别达到11.23 g/瓶和10.23。
- Abstract:
- In this paper, the subculture of soybean were studied from the aspects of basic media, culturing conditions, phytohormones etc.The optimum conditions for soybean callus subculture were as follows: Growth status of callus was better on MS medium supplemented with BA0.5 mg/L, 2,4-D2.0 mg/L, the callus yield and increasing times were the highest, it can reached 11.23 g/Flask and 10.23. The suitable pH value of medium was among 5.8~6.0. The callus cultured in light(1000~1500Lx,12 h/d) for 21d were suitable for the callus growth.
参考文献/References:
[1]徐刚标,何方,陈良昌.银杏愈伤组织诱导与继代培养的研究[J].中南林学院学报,1999,19(3):32-36.
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983~2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(02):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(02):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(02):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(02):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(02):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(02):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(02):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(02):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(02):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(02):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]孙明杰,魏玄,邹丹丹,等.大豆子叶节高频愈伤组织诱导及其植株再生[J].大豆科学,2011,30(04):546.[doi:10.11861/j.issn.1000-9841.2011.04.0546]
SUN Ming-jie,WEI Xuan,ZOU Dan-dan,et al.High Frequency Induction of Callus from Soybean Cotyledonary Node Explants and Its Plant Regeneration[J].Soybean Science,2011,30(02):546.[doi:10.11861/j.issn.1000-9841.2011.04.0546]
[12]张庆林,赵艳,张艳,等.不同基因型大豆愈伤组织对农杆菌EHA105的敏感性研究[J].大豆科学,2011,30(04):566.[doi:10.11861/j.issn.1000-9841.2011.04.0566]
ZHANG Qing-lin,ZHAO Yan,ZHANG Yan,et al.Sensitivity of Different Genotype Soybean Callus to Agrobacterium EHA105[J].Soybean Science,2011,30(02):566.[doi:10.11861/j.issn.1000-9841.2011.04.0566]
[13]葛正珍,杨光宇,仲艳,等.硼对大豆愈伤组织的细胞结构及形态的影响[J].大豆科学,2009,28(02):225.[doi:10.11861/j.issn.1000-9841.2009.02.0225]
GE Zheng-zhen,YANG Guang-yu,ZHONG Yan,et al.Effect of Boron on Cell Structure and Shape in Soybean(Glycine max)Callus[J].Soybean Science,2009,28(02):225.[doi:10.11861/j.issn.1000-9841.2009.02.0225]
[14]李文霞,李文滨,吕文河,等.农杆菌介导大豆子叶节系统的两个问题突破[J].大豆科学,2008,27(01):173.[doi:10.11861/j.issn.1000-9841.2008.01.0173]
LI Wen-xia,LI Wen-bin,Lv Wen-he,et al.Breakthrough of Two Questions on the Agrobacterium-mediated Soybean Cotyledonary Node Systems[J].Soybean Science,2008,27(02):173.[doi:10.11861/j.issn.1000-9841.2008.01.0173]
[15]张郁松.影响大豆愈伤组织的诱导因素研究[J].大豆科学,2007,26(01):66.[doi:10.3969/j.issn.1000-9841.2007.01.015]
ZHANG Yu-song.RESEARCH ON AFFECTIVE FACTOR OF SOYBEAN CALLUS INDUCTION[J].Soybean Science,2007,26(02):66.[doi:10.3969/j.issn.1000-9841.2007.01.015]
[16]苏彤,姚陆铭,张鑫,等.大豆愈伤原生质体的制备和培养方式探究[J].大豆科学,2018,37(05):741.[doi:10.11861/j.issn.1000-9841.2018.05.0741]
SU Tong,YAO Lu-ming,ZHANG Xin,et al.Study on the Preparation and Cultivation of Soybean Callus Protoplasts[J].Soybean Science,2018,37(02):741.[doi:10.11861/j.issn.1000-9841.2018.05.0741]
备注/Memo
作者简介:张郁松(1974-),男,讲师,硕士,研究方向生物制药。E-mail:zhangyusong123@126.com