[1]邱承祥　武天龙.6 -BA 对大豆茎尖诱导再生植株的研究[J].大豆科学,2003,22(01):32-35.[doi:10.11861/j.issn.1000-9841.2003.01.0032]
　Qiu Chengxiang Wu Tianlong.STUDY ON 6 -BA TO THE REGENERATION OF TIP SHOOT OF SOYBEAN[J].Soybean Science,2003,22(01):32-35.[doi:10.11861/j.issn.1000-9841.2003.01.0032]
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6 -BA 对大豆茎尖诱导再生植株的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第22卷 期数: 2003年01期 页码: 32-35 栏目: 出版日期: 2003-02-25
Title:
STUDY ON 6 -BA TO THE REGENERATION OF TIP SHOOT OF SOYBEAN
文章编号:
1000 -9841(2003)01 -0032 -04
作者:
邱承祥　武天龙
上海交通大学上海201101
Author(s):
Qiu Chengxiang 　Wu Tianlong
Shanghai Jiaotong University , Shanghai 201101
关键词:
大豆; 茎尖; 6 -BA ; 植株再生
Keywords:
Soybean ; Tipshoot ; 6 -BA ; Regeneration
分类号:
S 565.035.3
DOI:
10.11861/j.issn.1000-9841.2003.01.0032
文献标志码:
A
摘要:
以大豆茎尖作为材料, 采用MSB5(MS无机+B5 有机)培养基附加不同浓度的6 -BA 诱导不定芽分化, 建立了以茎尖为外植体的大豆组培高效再生植株体系。不同品种研究结果表明:预培养时的6 -BA 浓度对不定芽分化的数量以及芽的伸长有直接影响, 低浓度的6 -BA 产生芽数量少, 变异系数小, 有利于不定芽的伸长, 高浓度的6 -BA 产生芽数量多, 变异系数也小, 不易伸长, 在3 .0mgL 浓度的6 -BA 处理比较理想, 变异系数较大, 有效芽多。不同品种对6 -BA 的耐受程度也存在一定差异, 合适的6 -BA 浓度有使差异减小的趋势。
Abstract:
Tip shoot of soybean (Glycin max L .)were selected as explant and precultured in MSB (inorganic of MS +organic of B5)medium with different concentration of 6 -BA to inducing buds .The high frequency regeneration system oftissue culture of soybean was established .The result indicated that concentration of 6 -BA of preculture had a direct influenceto the number of buds and elongating of buds .Low concentration of 6 -BA produced a few buds , and the coeffecientof variability was small , but benefit to buds’ elongation ;high concentration of 6 -BA producdmany buds , the coeffecientof variability was small too , but not easy elongation .The treatment with 3.0mg L of 6 -BA was better , the coeffecientof variability was large , effective buds were more .Ditterent soybean varietes had difterent responses to the concentrationof 6 -BA , high concentration of 6 -BA tended to reduce the difference .

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备注/Memo

作者简介:邱承祥(1975-), 男, 硕士, 研究方向遗传转化。

更新日期/Last Update: 2016-12-31