[1]刘金华王丕武武丽敏于彦春张君郝文媛姚丹.脯氨酸、硝酸银对农杆菌转化大豆的影响[J].大豆科学,2003,22(01):36-39.[doi:10.11861/j.issn.1000-9841.2003.01.0036]
　Liu JinhuaWang PeiwuWu LiminYu YanchunZhan JunHao WenyuanYao Dan.EFFECTION OF PROLINE AND AgNO3 ON AGROBACTERIUM-MEDIATEDTRANSFORMATION OF SOYBEAN[J].Soybean Science,2003,22(01):36-39.[doi:10.11861/j.issn.1000-9841.2003.01.0036]
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脯氨酸、硝酸银对农杆菌转化大豆的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第22卷 期数: 2003年01期 页码: 36-39 栏目: 出版日期: 2003-02-25
Title:
EFFECTION OF PROLINE AND AgNO3 ON AGROBACTERIUM-MEDIATEDTRANSFORMATION OF SOYBEAN
文章编号:
1000 -9841(2003)01 -0036 -04
作者:
刘金华1王丕武2武丽敏2于彦春2张君2郝文媛2姚丹2
1 .吉林出入境检验检疫局技术中心长春130062 ;
2.吉林农业大学生物技术中心长春130118
Author(s):
Liu Jinhua1Wang Peiwu2Wu Limin2Yu Yanchun2Zhan Jun2Hao Wenyuan2Yao Dan2
1 .Center of Technology , Jilin Entry -Exit Inspection and Quarantine Bureau of the P .R .CChangchun 130062 ;
2 .Center of Biotechnology , Jilin AgriculturalUniversity , Changchun 130118)
关键词:
大豆; 农杆菌; 遗传转化; 脯氨酸; 硝酸银
Keywords:
Soybean ; Agrobacterium tumefaciens ; Genetic transformation ; Proline ; AgNO3
分类号:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2003.01.0036
文献标志码:
A
摘要:
农杆菌介导的遗传转化技术是植物遗传转化中广泛采用的一种技术。如何提高转化率是植物遗传转化中的一个关键问题。已发现有许多物质能够促进农杆菌介导的遗传转化, 如乙酰丁香酮(AS)等许多酚类化合物都有助于农杆菌T -DNA 的转移从而提高转化率。本实验在农杆菌转化大豆的过程中辅加化合物脯氨酸、硝酸银, 通过对GUS 基因表达的分析, 结果表明这两种化合物均能明显地提高大豆的转化率, 其中2mg L 浓度的脯氨酸及2mg L 的AgNO3 具有最佳效果, 较适合用于大豆的转化。
Abstract:
Agrobacterium tumefaciens has been used extensively for genetic engineering in soybean , but to date , transformationfrequency of soybean is still low relative to that obtained in other plants .At an attempt to improve transformationefficiency , present experiment was conducted to add two compounds on transformation of cotyledonary nodes in soybeanvia Agrobacterium -mediation .The experiment result indicated that 2mg L proline or 2mg L AgNO3 had the optimal effect on soybean transformation, the GUS gene tansformation rate was increased obviously .

参考文献/References:

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4 　Jefferson RA ,TA Kavanagh , WM Bevan.GUS fusions :(X-glu -curonidase)as a sensitive and versatile gene -fusion marker in higher plants[ J] .EMBOJ .1987 , 6 :3901 -3907.
5 　蓝海燕, 陈正华.农杆菌介导的芸薹属植物遗传转化技术的研究进展[ J] .生物技术通报, 1999, 3 :15 -18.
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备注/Memo

基金项目:“ 国家转基因植物中试与产业化基地建设” 专项基金资助项目(J99 -13 -001)　

作者简介:刘金华(1975-), 男, 硕士研究生, 主要从事大豆遗传转化及基因检测工作。

更新日期/Last Update: 2016-12-31