[1]李海燕,武小霞,刘淼,等.大豆子叶节、胚尖再生植株的研究[J].大豆科学,2007,26(05):709-712.[doi:10.3969/j.issn.1000-9841.2007.05.014]
　LI Hai-yan,WU Xiao-xia,LIU Miao,et al.PLANT REGENERATION FROM COTYLDEONARY NODES AND EMBRYONIC TIPS OF SOYBEAN[J].Soybean Science,2007,26(05):709-712.[doi:10.3969/j.issn.1000-9841.2007.05.014]
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大豆子叶节、胚尖再生植株的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年05期 页码: 709-712 栏目: 出版日期: 2007-10-25
Title:
PLANT REGENERATION FROM COTYLDEONARY NODES AND EMBRYONIC TIPS OF SOYBEAN
文章编号:
1000-9841(2007)05-0709-04
作者:
李海燕; 武小霞; 刘淼; 韩英鹏
东北农业大学大豆研究所，教育部大豆生物学重点实验室，哈尔滨，150030
Author(s):
LI Hai-yan; WU Xiao-xia; LIU Miao; HAN Ying-peng
Soybean Research Institute of Northeast Agricultural University，Key Laboratory of Soybean Biology in Chinese Ministry of Education，Harbin 150030
关键词:
大豆; 子叶节; 茎尖; 激素; 再生
Keywords:
Soybean; Cotyledonary node; Stem tip; Hormone; Regeneration
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.05.014
文献标志码:
A
摘要:
为了获得高效的大豆再生体系，以大豆品种合丰35和北京小黑豆的成熟种子为材料，利用氯气和升汞两种消毒方法，研究不同浓度6-BA和2,4-D对子叶节和茎出芽的影响。结果表明：在大豆萌发时，加入 1.0 mg L-16-BA和 2.0 mg L-12,4-D，每个外植体分化的芽数较高；在茎尖再生系统中，6-BA和2,4-D诱导茎尖不定芽形成的最佳浓度是3.0 mg L-1和3.5 mg L-1，最佳诱导时间是24-48 h。
Abstract:
In order to obtain high efficiency soybean regeneration system，cotyledonary nodes and stem tips of soybean Hefeng 35 and Peking was used as explants.The disinfect ways of mercuric chloride and chlorine gas were compared，and the effect of 6-BA and 2,4-D on the regeneration of soybean cotyledonary node and stem tip were investigated.The results showed chlorine gas was a better disinfection method.The best concentration of 6-BA and 2,4-D was 1.0 and 2.0 mg L-1in germination medium respectively.The optimum concentration of 6-BA and 2,4-D was 3.0 and 3.5 mg L-1in regeneration of stem tips.And the best inducing time was 24-28 h.

参考文献/References:

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备注/Memo

基金项目：大豆生物学教育部重点实验室主任基金项目(SB07A04)

作者简介：李海燕(1978-)，女，研究实习员，主要从事大豆基因工程方面的研究。Tel: 0451-55191945; E-mail: lhy2112002@yahoo.com.cn

更新日期/Last Update: 2014-10-20