[1]曹乐生,王晗慧,张天旭,等.大豆Argonaute4的生物信息学分析[J].大豆科学,2019,38(01):25-34.[doi:10.11861/j.issn.1000-9841.2019.01.0025]
　CAO Le-sheng,WANG Han-hui,ZHANG Tian-xu,et al.Bioinformatics Analysis of Soybean Argonaute4[J].Soybean Science,2019,38(01):25-34.[doi:10.11861/j.issn.1000-9841.2019.01.0025]
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大豆Argonaute4的生物信息学分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷 期数: 2019年01期 页码: 25-34 栏目: 出版日期: 2019-01-20
Title:
Bioinformatics Analysis of Soybean Argonaute4
作者:
曹乐生; 王晗慧; 张天旭; 解莉楠
（东北林业大学 生命科学学院，黑龙江 哈尔滨 150040)
Author(s):
CAO Le-sheng;  WANG Han-hui;  ZHANG Tian-xu;  XIE Li-nan
（School of Life Science, Northeast Forestry Univesity, Harbin 150040, China）
关键词:
大豆; AGO4; 生物信息学; DNA甲基化
Keywords:
Soybean;  AGO4;  Bioinformatics;  DNA methylation
DOI:
10.11861/j.issn.1000-9841.2019.01.0025
文献标志码:
A
摘要:
在RNA介导的DNA甲基化途径（RNA-directed DNA methylation pathway）中，Argonaute4（AGO4）蛋白是DNA被甲基化修饰的关键蛋白。研究大豆中Argonaute4蛋白能够为大豆甲基化研究奠定基础，本研究参考NCBI、Phytozome、UniProt KB等网站及数据库，对拟南芥的AGO4基因进行同源性分析，确定了大豆中AGO4蛋白及其基因序列，并对其进行分析预测。研究发现，大豆中AGO4包括3个基因GmAGO4A、GmAGO4B、GmAGO4C，它们的一级结构、二级结构及理化性质都很相似，三级结构有差异，大豆AGO4C与拟南芥AGO4结构更相似。在302个野生大豆品系中对GmAGO4的3个基因的外显子进行核酸及蛋白序列的分析，发现一些大豆品系的蛋白质由于突变三维结构发生了变化。对大豆中AGO4及其参与的甲基化途径的研究具有指导意义。
Abstract:
In the RNA-directed DNA methylation pathway, the Argonaute4 (AGO4) protein plays a key role in DNA methylation. In order to study Argonaute4 protein in soybean and lay a foundation for the study of methylation in soybean, in this paper, homology analysis of AGO4 gene in Arabidopsis thaliana was performed with NCBI, Phytozome, UniProt KB and other websites, moreover, AGO4 protein and its gene sequence in soybean were confirmed and analyzed. The results indicate that there are three homologous genes of AtAGO4 in soybean, GmAGO4A, GmAGO4B, GmAGO4C respectively, which primary structures, secondary structures and physicochemical property are similar, but the tertiary structures are different, the tertiary structures of GmAGO4C and Arabidopsis AGO4 are more similar. The nucleic acid and protein sequences of the three genes’ exons were analyzed in 302 wild soybean lines, and it was found that the tertiary structures of some soybean lines changed due to mutation.The results of this paper will be helpful for further study on the function of AGO4 in the methylation pathway in soybean.

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备注/Memo

收稿日期：2018-08-03

基金项目：中央高校基本科研业务费专项资金项目（2572017DA06，2572014CA21）。
第一作者简介：曹乐生（1997-），男，学士，主要从事大豆表观遗传学调控因子研究。E-mail：caolesheng@nefu.edu.cn。
通讯作者：解莉楠（1978-），女，博士，副教授，主要从事植物抗逆生物学研究。E-mail：linanxie@nefu.edu.cn。

更新日期/Last Update: 2019-01-22