[1]王迪,邱红梅,王曙明,等.大豆2S清蛋白基因生物信息学分析[J].大豆科学,2019,38(01):16-24.[doi:10.11861/j.issn.1000-9841.2019.01.0016]
　WANG Di,QIU Hong-mei,WANG Shu-ming,et al.Comparative Analysis of Soybean 2S Albumin Gene[J].Soybean Science,2019,38(01):16-24.[doi:10.11861/j.issn.1000-9841.2019.01.0016]
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大豆2S清蛋白基因生物信息学分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷 期数: 2019年01期 页码: 16-24 栏目: 出版日期: 2019-01-20
Title:
Comparative Analysis of Soybean 2S Albumin Gene
作者:
王迪1; 2; 邱红梅2; 王曙明2; 高淑芹2; 马晓萍2; 王洋2; 郑大浩1; 王跃强2
（1.延边大学，吉林 延吉 133000； 2.吉林省农业科学院 大豆研究所/大豆国家工程研究中心，吉林 长春 130033）
Author(s):
WANG Di1; 2;  QIU Hong-mei2;  WANG Shu-ming2;  GAO Shu-qin2;  MA Xiao-ping2;  WANG Yang2;  ZHENG Da-hao1;  WANG Yue-qiang2
(1.Yanbian University, Yanji 133000, China; 2.Soybean Research Institute/National Engineering Research Center for Soybean, Jilin Academy of Agricultural Sciences, Changchun 130033, China)
关键词:
大豆; LTP; 进化分析; 差异表达
Keywords:
Soybean; LTP; Evolutionary analysis; Differential expression
DOI:
10.11861/j.issn.1000-9841.2019.01.0016
文献标志码:
A
摘要:
2S清蛋白含有动物胰蛋白酶抑制剂及过敏原等多种具有生理活性的多肽，可引起特殊人群尤其是儿童的过敏反应，其编码基因为LTP基因家族。为系统地分析2S清蛋白编码基因，利用生物信息学分析方法获得2S清蛋白基因的全序列、定位以及转录本等信息，鉴定了30个大豆LTP家族基因。基因定位结果表明：23.33%基因在染色体上串联排布，反映了该家族基因的串联重复进化。通过基因功能注释得到22个非特异性脂质转运蛋白，1个类伸展素蛋白，4个脯氨酸蛋白，2个种子储存2S清蛋白和1个雄蕊特殊蛋白。根据系统发育分析将这些蛋白分成3个类群。类群I有类伸展素蛋白、脯氨酸蛋白、雄蕊特殊蛋白和种子储存2S清蛋白，类群II有16个非特异性脂质转运蛋白，类群III有6个非特异性脂肪转移蛋白。种子储存2S清蛋白基因在种子发育中后期特异表达，表达量显著高于其它同源基因。本研究为进一步研究其功能提供指导，为该类基因应用于大豆品质改良提供理论支撑。
Abstract:
2S albumin derived from the LTP gene family contains various physiologically active peptides such as animal trypsin inhibitors and allergens, which can cause allergic reactions in special populations, especially in children. In order to systematically analyze the encoding gene of 2S albumin, this study used bioinformatics analysis to obtain the full sequence, localization and transcript information of the 2S albumin gene, identified 30 soybean LTP family genes. The results of gene mapping indicated that 23.33% of the genes were arranged in tandem on the chromosome, reflecting the tandem repeat evolution of the family genes. And there are 22 non-specific lipid transporters, 1 extensin-like protein, 4 proline proteins, 2 seeds, 2S albumin and 1 stamen-specific proteins were obtained by gene function annotation. These proteins were divided into three groups based on phylogenetic analysis. Group I has extensin-like proteins, proline proteins, stamen-specific proteins and seeds to store 2S albumin, group II has 16 non-specific lipid transporters, and group III has 6 non-specific fat transfer proteins. The 2S albumin gene was specifically expressed in the late stage of seed development, and its expression level was significantly higher than other homologous genes,which provided guidance for further study of its function and theoretical support for the application of this gene in soybean quality improvement.

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备注/Memo

收稿日期：2018-08-09

基金项目：七大农作物育种专项（2016YFD0100201）；国家自然科学基金（31671712）；吉林省科技发展计划（20160414049GH）。
第一作者简介：王迪(1993-), 男, 硕士, 主要从事大豆基因挖掘研究。E-mail:549869052@qq.com。
通讯作者：郑大浩（1963-），男，博士，教授，主要从事作物遗传与育种研究。E-mail:ljzdhdh＠163.com；
王跃强（1968-），男，博士，研究员，主要从事大豆资源与遗传育种研究。E-mail:825164942@qq.com。

更新日期/Last Update: 2019-01-22