[1]孙雪慧,蔡勤安,尚丽霞,等.耐旱基因cspB转化大豆的研究[J].大豆科学,2017,36(05):699-704.[doi:10.11861/j.issn.1000-9841.2017.05.0699]
　SUN Xue-hui,CAI Qin-an,SHANG Li-xia,et al.Genetic Transformation of Soybean with Drought Tolerance Gene cspB from Bacillus subtilis[J].Soybean Science,2017,36(05):699-704.[doi:10.11861/j.issn.1000-9841.2017.05.0699]
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耐旱基因cspB转化大豆的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年05期 页码: 699-704 栏目: 出版日期: 2017-09-20
Title:
Genetic Transformation of Soybean with Drought Tolerance Gene cspB from Bacillus subtilis
作者:
孙雪慧1; 蔡勤安2; 尚丽霞2; 马瑞2; 杨向东2; 于志晶2; 魏建1
（1.长春师范大学，吉林 长春 130032; 2.吉林省农业科学院 生物技术研究所/吉林省农业生物技术重点实验室，吉林 长春 130033）
Author(s):
SUN Xue-hui1; CAI Qin-an2; SHANG Li-xia2; MA Rui2;  YANG Xiang-dong2;  YU Zhi-jing2; WEI Jian1
（1.Changchun Normal University, Changchun 130032, China; 2.Agro-Biotechnology Research Institute / Jilin Provincial Key Laboratory Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China）
关键词:
大豆; 耐旱; cspB基因; 遗传转化
Keywords:
Soybean;  Drought tolerance;  cspB- gene;  Genetic transformation
DOI:
10.11861/j.issn.1000-9841.2017.05.0699
文献标志码:
A
摘要:
为提高大豆的耐旱性，本研究根据大豆偏爱的密码子将来源于枯草杆菌抗旱相关基因cspB序列进行了优化，并构建了植物表达载体pCAMBIA3301-cspB，利用农杆菌介导法将cspB基因导入大豆栽培品种Bert 中。用于遗传转化子叶节外植体共3 800个，经PCR和Southern鉴定，并结合PPT抗性筛选，共获得了95棵阳性转基因植株，转化率为2.5%。经初步筛选，获得了7份耐旱性较好的转基因大豆新材料。这些材料将进一步用于耐旱转基因大豆新品种培育工作。
Abstract:
To improve the drought tolerance of soybean, a vector pCAMBIA3301-cspB with drought tolerance related modified gene -cspB, was constructed.The modified-cspB gene was introducted into soybean variety Bert by Agrobacterium-mediated transformation. In this experiment total-3 800-cotyledonary node explants were infected and 95 independent transgenic plants were obtained by PCR and Southern blot analysis combined with PPT selection.The transformation efficiency was 2.5%. Seven transgenic plants with drought tolerance were selected，which could be used for soybean drought tolerance breeding in further.

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备注/Memo

基金项目：转基因生物新品种培育重大专项(20148X0800403B-002)；吉林省科技厅重点科技攻关项目（20130206005NY）。

第一作者简介：孙雪慧（1992-），女，硕士，主要从事植物遗传研究。E-mail:2433003118@qq.com。
通讯作者：于志晶（1977-），女，硕士，副研究员，主要从事植物分子生物学、遗传转化与代谢工程研究。Email:yuzhijing0001@163.com;魏建（1980-），男，博士，教授，主要从事植物遗传研究。E-mail:148050459@qq.com。

更新日期/Last Update: 2017-10-29