[1]陈薇,王涛,李冬梅,等.大豆脯氨酸生物合成相关基因Glyma01g24530的功能预测及表达分析[J].大豆科学,2017,36(05):705-709.[doi:10.11861/j.issn.1000-9841.2017.05.0705]
　CHEN Wei,WANG Tao,LI Dong-mei,et al.Functional Prediction and Expression Analysis of Proline Synthetase Related Gene Glyma01g24530[J].Soybean Science,2017,36(05):705-709.[doi:10.11861/j.issn.1000-9841.2017.05.0705]
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大豆脯氨酸生物合成相关基因Glyma01g24530的功能预测及表达分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年05期 页码: 705-709 栏目: 出版日期: 2017-09-20
Title:
Functional Prediction and Expression Analysis of Proline Synthetase Related Gene Glyma01g24530
作者:
陈薇; 王涛; 李冬梅; 李悦; 李永光; 李文滨
（东北农业大学 大豆研究所/大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室, 黑龙江 哈尔滨 150030)
Author(s):
CHEN Wei;  WANG Tao;  LI Dong-mei;  LI Yue;  LI Yong-guang;  LI Wen-bin
(Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Soybean Biology and Breeding(Genetics) of Chinese Agriculture Ministry, Soybean Research Institute, Northeast Agricultural University, Harbin 150030, China)
关键词:
大豆;  脯氨酸; Glyma06g-28890; 植物抗逆
Keywords:
Soybean;  Proline;  Glyma06g-28890;  Plant stress resistance
DOI:
10.11861/j.issn.1000-9841.2017.05.0705
文献标志码:
A
摘要:
干旱胁迫下的脯氨酸积累在许多植物中都存在，人们普遍认为脯氨酸含量的提高促进了植物对干旱胁迫的抵抗能力。拟南芥中研究发现，△1-吡咯啉-5-羧酸合成酶基因P5CS1是一种限速酶用来催化脯氨酸生物合成，对脯氨酸非常重要。本研究通过生物信息学及荧光定量PCR对大豆中的P5CS1同源基因Glyma01g24530进行了初步的功能分析和表达模式验证。结果发现：Glyma01g24530具有保守的N端乙酰谷氨酸合成酶激酶结构域和N端乙酰谷氨酸合成酶激酶结构域，系统进化树显示与各植物中P5CS家族成员相似性很高，启动子顺式作用元件序列分析表明该基因存在逆境胁迫、光反应等元件。表达模式分析显示Glyma01g24530可以被干旱、盐诱导表达，并在多数组织中表达。
Abstract:
Free proline can accumulate to high levels under drought-stress in many plants, and it is widely acknowledged that the increase of proline content could promote the plant resistant to drought stress.The study in Arabidopsis thaliana found that △1pyrroline-5-carboxylate synthetase (P5CS) catalyzed the rate.limiting enzyme in the biosynthesis of proline and played an important role in the biosynthesis of proline.In this study, function and expression of P5CS1 homologous gene in soybean Glyma01g-24530 was analyzed through bioinformatics and real-time quantitative PCR.We found that Glyma01g-24530 had conservative N.acetylglutamate synthase, kinase-like domain and Aldehyde dehydrogenase domain structure Phylogenetic tree showed that Glyma01g-24530 had high similarity with P5CS1 family members in many plants.We also found that there were multiple cis-elements about abiotic stress responses, light responsiveness in its promoter sequence region through the statistics of promoter cis-elements. Expression pattern analysis showed Glyma01g-24530 could be induced by drought and salt, it could express in many tissues.

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备注/Memo

基金项目：黑龙江省普通本科高等学校青年创新人才培养计划（UNPYSCT2016005）；抗逆转基因大豆新品种培育(2016ZX08004002)。

第一作者简介：陈薇（1991-），女，硕士，主要从事作物遗传育种研究。E-mail：573972148qqcom@sina.com。
通讯作者：李文滨（1958-），男，教授，博导，主要从事作物遗传育种研究。E-mail：wenbinli@neau.edu.cn。

更新日期/Last Update: 2017-10-29