[1]李娜,于希森,李琪,等.大豆异黄酮合成关键酶基因对蚜虫取食的防御响应分析[J].大豆科学,2016,35(05):800-804.[doi:10.11861/j.issn.1000-9841.2016.05.0800]
　LI Na,YU Xi-sen,LI Qi,et al.The Analysis of Key Gene of Soybean Isoflavone Synthesis Response to Aphid Feeding Defense[J].Soybean Science,2016,35(05):800-804.[doi:10.11861/j.issn.1000-9841.2016.05.0800]
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大豆异黄酮合成关键酶基因对蚜虫取食的防御响应分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年05期 页码: 800-804 栏目: 出版日期: 2016-09-20
Title:
The Analysis of Key Gene of Soybean Isoflavone Synthesis Response to Aphid Feeding Defense
作者:
李娜; 于希森; 李琪; 李涵哲; 李洋; 徐婷婷; 孟凡立
东北农业大学大豆生物学省部教育部重点实验室/东北农业大学农业部东北大豆生物学与遗传育种重点实验室，黑龙江 哈尔滨 150030
Author(s):
LI Na;  YU Xi-sen;  LI Qi;  LI Han-zhe;  LI Yang;  XU Ting-ting;  MENG Fan-li
（1.Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin 150030, China; 2. Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin 150030, China）
关键词:
大豆; 异黄酮; 关键基因; 大豆蚜虫; 防御响应
Keywords:
Soybean;  Isoflavones;  Key Genes;  Soybean Aphid;  Defense Response
DOI:
10.11861/j.issn.1000-9841.2016.05.0800
文献标志码:
A
摘要:
以蚜虫差异抗性的大豆品种PI567598B（高抗）、绥农30（抗）、东农51（中抗）和Williams 82（感）为试验材料, 分别于接种大豆蚜虫 0，7，14和21 d时采集大豆叶片，利用qPCR对异黄酮合成关键酶基因苯丙氨酸解氨酶（PAL）、黄烷酮-3-3羟化酶（F3H）、大豆异黄酮合成基因1 (ISF1) 和大豆异黄酮合成基因2 (ISF2)的表达量进行分析。结果表明：蚜虫取食前高抗品种PI567598B中PAL、F3H、ISF1和ISF2-基因的相对表达量均高于感虫品种Williams 82；蚜虫取食后，抗蚜品种PI567598B和绥农30中PAL-基因在蚜虫取食14 d时显著上调，在21 d时达到最大，F3H-基因7 d时表达量最高，然后降低；IFS2表达量于蚜虫取食7 d后显著上调，在21 d时达到最大；中抗品种东农51中PAL、IFS2和F3H基因表达量在7 d时增加，但不显著；而感蚜品种Williams 82 蚜虫取食后PAL和IFS2表达量增加但不显著，且相对表达量显著低于抗蚜品种，F3H基因表达量不增反降；抗感大豆品种蚜虫取食后IFS1-基因表达均诱导不显著。研究表明,感蚜大豆品种蚜虫取食前后异黄酮合成关键酶基因表达量都比较低，而抗虫品种异黄酮合成关键酶基因表达量高，且防御响应持续时间长，符合其抗性差异。
Abstract:
In order to elucidate isoflavone mechanisms of the response to aphid in the soybean plants, different-resistant soybean varieties PI567598B (high), Suinong 30 (medium), Dongnong 51(low) and Williams 82 (flu), were inoculated by soybean aphid for 0, 7, 14 and 21 days.Using qPCR, the expression patterns of key enzyme genes were analyzed in response to aphid feeding defense, including Phenylalanine ammonia lyase (PAL), Flavanone hydroxylase -3-3 (F3H), Isoflavone synthase gene 1 (ISF1) and Isoflavone synthase gene 2 (ISF2).The results showed leaves from PI567598B had higher -PAL, F3H, ISF1 and ISF2 expression than Williams 82 without aphid attack. The PAL gene in PI567598B and Suinong 30 was increased significantly after inoculated 14 d and reached highest level at 21 d. The F3H gene was increased rapidly at 7 d and then showed a decreasing trend; the IFS2 gene was increased rapidly and the highest in 24 h.The PAL, IFS2 and F3H genes in Dongnong 51 were not induced significantly after inoculated 7 d.The PAL and IFS2 genes in Williams 82 were not induced significantly and lower than other three lines even after being damaged by aphid, while gene expression of F3H decreased after inoculation.The expression of IFS1 gene was not induced in four lines after inoculation. These result showed that the expression level of isoflavone key enzyme gene were higher and longer in resistant varieties than that in susceptible soybean varieties, which was consistent with its resistance differences.

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备注/Memo

基金项目：国家自然科学基金青年基金（31201229）；转基因大豆新品种培育重大专项 (2011ZX08004-004)；中国博士后启动基金（2012M510914）；黑龙江省博士后资助项目（LBH-Z11223）； 东北农业大学博士启动基金（2012RCB07）。第一作者简介：李娜（1990-），女，硕士，主要从事大豆遗传育种研究。E-mail:a01100187@163.com。通讯作者：孟凡立（1978-），女，博士，副教授，主要从事大豆遗传育种研究。E-mail：fanli78@sina.com。

更新日期/Last Update: 2016-09-25