[1]孙君明,韩粉霞,闫淑荣,等.高异黄酮低豆腥味大豆新品种中黄68的选育[J].大豆科学,2015,34(05):900-905.[doi:10.11861/j.issn.1000-9841.2015.05.0900]
　SUN Jun-ming,HAN Fen-xia,YAN Shu-rong,et al.Development of a Novel Soybean Cultivar Zhonghuang 68 with High Isoflavone Content and Low Off-Flavor[J].Soybean Science,2015,34(05):900-905.[doi:10.11861/j.issn.1000-9841.2015.05.0900]
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高异黄酮低豆腥味大豆新品种中黄68的选育

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年05期 页码: 900-905 栏目: 出版日期: 2015-10-25
Title:
Development of a Novel Soybean Cultivar Zhonghuang 68 with High Isoflavone Content and Low Off-Flavor
作者:
孙君明; 韩粉霞; 闫淑荣; 杨华; 李斌
中国农业科学院 作物科学研究所/农业部大豆生物学重点实验室，北京 100081
Author(s):
SUN Jun-ming;  HAN Fen-xia;  YAN Shu-rong;  YANG Hua;  LI Bin
Institute of Crop Science, Chinese Academy of Agricultural Sciences /Ministry of Agriculture (MOA) Key Laboratory of Soybean Biology, Beijing 100081, China
关键词:
大豆; 新品种; 中黄68; 高异黄酮; 低豆腥味
Keywords:
Soybean;  Novel cultivar;  Zhonghuang 68;  High isoflavone content;  Low off-flavor
DOI:
10.11861/j.issn.1000-9841.2015.05.0900
文献标志码:
A
摘要:
高异黄酮大豆品种中黄68系中国农业科学院作物科学研究所以缺失脂肪氧化酶-2基因（lx-2）的低豆腥味大豆品种中黄18为母本，日本引进的7S蛋白亚基缺失（α’亚基缺失和β亚基含量低）的大豆材料7S3（Karikoi-434）为父本，经有性杂交，采用高效液相色谱（HPLC）技术检测后代籽粒的异黄酮含量，等电聚焦聚丙烯酰胺凝胶电泳（IEF-PAGE）技术检测脂肪氧化酶基因缺失，并进行多年生化标记辅助选择而成。该品种2013年通过北京市品种审定委员会审定，其突出特点是高产、稳产、异黄酮含量高（5 135.86 mg.kg-1）、缺失脂肪氧化酶-2基因（lx-2）、商品性好、综合性状优良，属高异黄酮含量低豆腥味的特用大豆新品种。
Abstract:
A novel soybean cultivar-Zhonghuang 68 was developed in the Institute of Crop Science, CAAS, which derived from cv. Zhonghuang 18 (female parent, null lipoxygenase-2 gene) and line 7S3 (Male parent, Karikoi-434, null α’-subunit and low content of β-subunit). This cultivar was developed by the biochemical marker assistant selection using High Performance Liquid Chromatography (HPLC) method for isoflavone content and Isoelectric Focusing-Polyacrylamide Gel Electrophoresis (IEF-PAGE) method for lipoxygenase genes.In 2013, this variety was passed the examination and approved by Beijing Committee of Crop Variety Examination and Approval because of its outstanding characteristics, such as high and stable yield, high isoflavone content (5 135.86 mg.kg-1), null lipoxygenase-2 (lx-2) gene, as well as good general characters.It belongs to the novel soybean variety with high isoflavone content and low off-flavor in China.

参考文献/References:

［1］Adlercreutz C H T, Goldin B R, Gorbach S L, et al. Soybean phytoestrogen intake and cancer risk ［J］. Journal of Nutrition, 1995, 125: 757S-770S

［2］Peterson T G, Barnes S.Genistein inhibition of the growth of human breast cancer cells: Independence from estrogen receptors and the multi-drug resistance gene ［J］.Biochemical Biophysical Research Communications, 1991, 179: 661-667
［3］Coward L, Barnes N C, Setchell K D R. Genistein, daidzein, and their glycoside conjugates: Antitumor isoflavones in soybean foods from American and Asian diets ［J］.Journal of Agricultural and Food Chemistry, 1993, 41: 1961-1967
［4］孙君明, 韩粉霞.植物次生代谢产物异黄酮的调控机理 ［J］. 西南农业学报, 2005, 18(5): 663-667.（Sun J M, Han F X. Manipulating mechanism of secondary metabolites-isoflavone in plants［J］. Southwest China Journal of Agricultural Sciences, 2005,18(5):663-667.）
［5］孙君明, 丁安林, 常汝镇, 等. 中国大豆异黄酮含量的初步分析 ［J］.中国粮油学报, 1995, 10(4): 51-54.(Sun J M, Ding A L, Chang R Z, et al. Preliminary analysis on soybean isoflavons content in China ［J］. Journa of the Chinese Cereals and Oils Association, 1995,10(4):51-54.)
［6］Kudou S, Fleury Y, Welti D, et al. Malonyl isoflavone glucosides in soybean seeds (Glycine max Merri) ［J］. Agricultural and Biological Chemistry, 1991, 55(9): 2227-2233.
［7］孙君明, 韩粉霞, 丁安林．高效液相色谱（HPLC）技术鉴定中国南方大豆品种异黄酮主要组分［J］．植物遗传资源学报, 2004, 23（4): 245-248.(Sun J M, Han F X, Ding A L. Determination of major isoflavone components based on HPLC technology in southern soybean varieties in China［J］.Journal of Plant Genetic Resources,2004,5(3):222-226.)
［8］孙君明, 丁安林, 沈黎明．光照对大豆幼苗组织中异黄酮含量和分布的影响 ［J］．植物学报, 1998, 40(11): 1015-1021.(Sun J M, Ding A L.Light effect on the tissue contents and distribution of isoflavones in the developing seedling of soybean［J］.Acta Botanica Sinica, 1998,40(11):1015-1021.)
［9］Kitamura K,Igita K, Kikuchi A, et al.Low isoflavone content in some early maturing cultivars, so-called “Summer type soybean” (Glycine max (L) MERRILL) ［J］.Japan Journal of Breed, 1991, 41: 651-654.
［10］林红, 来永才,齐宁,等.黑龙江省野生大豆、栽培大豆高异黄酮种质资源筛选 ［J］．植物遗传资源学报, 2005, 6(1): 53-55.(Lin H, Lai Y C, Qi N, et al.Screening of germplasm with high content of isoflavones in wild and cultivated soybean in Heilongjiang［J］. Journal of Plant Genetic Resources,2005,6(1):53-55.)
［11］来永才, 李炜, 王庆祥,等. 黑龙江省野生大豆高异黄酮新种质创新利用Ⅰ异黄酮含量及与籽粒相关性状的分析 ［J］．大豆科学, 2006, 25(4): 414-416.(Lai Y C, Li W, Wang Q X, et al. Innovation and utilization of new high isoflavone resource of wild soybean in Heilongjiang province I Analysis of isoflavone content and relevant of characters［J］.Soybean Science,2006,25(4):414-416.)
［12］李辉, 戴常军, 兰静,等. 黑龙江栽培大豆异黄酮含量的初步分析［J］.中国粮油学报, 2007, 22(1): 38-40.(Li H, Dai C J, Lan J, et al. Primary Analyse of Isoflavones content in Heilongjiang province soybean cultivars［J］. Journal of the Chinese Cereals and Oils Association, 2007,22(1):38-40.)
［13］葛一楠, 孙君明, 韩粉霞,等.代表性大豆种质异黄酮主要组分含量鉴定［J］. 植物遗传资源学报, 2011,12(6):921-927.(Ge Y N, Sun J M, Han F X, et al.Major isoflavone components in soybean seeds from the representative core samples［J］.Journal of Plant Genetic Resources, 2011, 12(6):921-927.)
?［14］Axelrod B.Lipoxygenase form soybeans ［J］. Meth enzymol, 1981, 71:441-451.
［15］韩粉霞, 孙君明, 丁安林, 等. 缺失Kunitz胰蛋白酶抑制剂和脂肪氧化酶2的大豆新品种中黄31的选育［J］.中国油料作物学报, 2006, 28(2): 210-213.(Han F X, Sun J M, Ding A L, et al.Development of a new soybean variety Zhonghuang 31 with null kunitz trypsin inhibitor and lipoxygenase 2 genes［J］.Chinese Journal of Oil Crop Sciences, 2006, 28(2): 210-213.)
［16］Eskin N A M.Biochemistry of lipoxygenase EC01,13,11,13 in relation to food quality ［J］.Critical Reviews in Food Science and Nutrition, 1977, 9:1-41.?
［17］Sun J M, Sun B L, Han F X, et al.Rapid HPLC method for determination of 12 isoflavone components in soybean seeds ［J］. Agricultural Science China, 2011, 10:70-77.
［18］丁安林, 傅翠真, 常汝镇, 等.大豆中脂肪氧化酶同工酶的鉴定研究［J］.作物学报, 1994, 20(3): 373-374.(Ding A L, Fu C Z, Chang R Z, et al.Study on identifying fatty oxidase isozymes in soybean ［J］.Acta Agronomica Sinica, 1994, 20(3): 373-374.)
［19］韩粉霞, 丁安林, 孙君明.高异黄酮含量大豆新品种中豆27的特征特性及栽培技术要点［J］. 大豆科学, 2002,21(3): 231-233.(Han F X, Ding A L, Sun J M. Development of a new soybean variety with high isoflavone Zhongdou 27 and its cultivation practices［J］.Soybean Science, 2002,21(3): 231-233.)
［20］朱洪德,谢甫娣, 费志宏. 高油高异黄酮大豆新品种垦农21的选育和配套栽培技术［J］. 种子, 2006(5):92-95.(Zhu H D, Xie F T, Fei Z H. Breeding and cultivation of new soybean variety Kennong 21 with high oil an high isoflavone［J］.Seeds, 2006(5):92-95.)
［21］朱洪德, 王树坤, 费志宏, 等. 高异黄酮大豆新品种垦鉴豆43的育成及配套高产栽培技术 ［J］. 黑龙江农业科学, 2006(3):14-16.(Zhu H D, Wang S K, Fei Z H, et al. Breeding and cultivation of new soybean variety Kenjiandou 43 with high isoflavone［J］. Heilongjiang Agricultural Sciences, 2006(3):14-16.)
［22］宁海龙, 李文滨, 李文霞,等. 高异黄酮含量大豆新品种东农51号的选育 ［J］.作物杂志, 2007(6): 96.(Ning H L, Li W B, Li W X, et al. Breeding of new soybean variety Dongnong 51 with high isoflavone ［J］. Crops, 2007(6): 96.)
［23］宁海龙, 李文滨, 李文霞,等.高油高异黄酮含量大豆新品种东农53号的选育 ［J］. 作物杂志, 2010(4): 104. (Ning H L, Li W B, Li W X, et al. Breeding of new soybean variety Dongnong 53 with high oil an high isoflavone［J］. Crops, 2010(4): 104.)
［24］李楠, 颜秀娟, 李明姝,等. 高异黄酮含量、高产大豆新品种吉育94的选育及栽培技术 ［J］. 吉林农业科学, 2009, 34(3):14-15.(Li N, Yan X J, Li M S, et al.Breeding and cultivation of new soybean variety Jiyu 94 with high yield an high isoflavone［J］. Jilin Agricultural Sciences, 2009, 34(3):14-15.)
［25］马巍, 王新风, 王巍巍,等. 高油高异黄酮春大豆新品种吉育97的选育 ［J］. 湖北农业科学, 2013(4):771-772.(Ma W, Wang X F, Wang W W, et al. Breeding of new soybean variety Jiyu 97 with high oil an high isoflavone［J］. Hubei Agricultural Sciences, 2013(4):771-772.)
［26］Zhang J Y, Ge Y N, Han F X, et al.Isoflavone content of soybean cultivars from maturity group 0 to VI grown in northern and southern China ［J］. Journal of American Oil Chemists Society, 2014, 91:1019-1028.
［27］孙君明, 韩粉霞, 丁安林.优质大豆新品种 ［J］. 大豆科学, 2002, 21(1): 81-82.(Sun J M, Han F X, Ding A L. Supreme new soybean variety［J］ Soybean Science, 2002, 21(1): 81-82.)

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备注/Memo

基金项目：国家“十二五”科技支撑计划（2014BAD11B01-X02）；中央级公益性科研院所基本科研业务费和中国农业科学院科技创新工程。

第一作者简介：孙君明（1972-），博士，研究员，主要从事大豆高产品质育种与栽培研究。E-mail：sunjunming@caas.cn。

更新日期/Last Update: 2015-11-09