[1]刘冬冬,王洋,柏锡.大豆Glyma08g02580及其同源蛋白的生物信息学分析[J].大豆科学,2014,33(05):648-653.[doi:10.11861/j.issn.1000-9841.2014.05.0648]
　LIU Dong-dong,WANG Yang,BAI Xi.Bioinformatics Analysis on Transcription Factor Glyma08g02580 and Its Homology Proteins[J].Soybean Science,2014,33(05):648-653.[doi:10.11861/j.issn.1000-9841.2014.05.0648]
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大豆Glyma08g02580及其同源蛋白的生物信息学分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年05期 页码: 648-653 栏目: 出版日期: 2014-10-25
Title:
Bioinformatics Analysis on Transcription Factor Glyma08g02580 and Its Homology Proteins
文章编号:
1000-9841.2014.05.0648
作者:
刘冬冬; 王洋; 柏锡
东北农业大学 生命科学学院，黑龙江 哈尔滨 150030
Author(s):
LIU Dong-dong; WANG Yang; BAI Xi
Life Science College of Northeast Agricultural University,Harbin 150030,China
关键词:
WRKY; 转录因子; 生物信息学; 系统进化分析; 基因表达谱
Keywords:
WRKY; Transcription factor; Bioinformatics; Phylogenetic analysis; Gene expression profile
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0648
文献标志码:
A
摘要:
WRKY蛋白是一个植物特有的转录因子，最古老的WRKY转录因子具有2个高度保守的WRKY结构域，大概起源于15亿～20亿年前的真核生物。虽然所有的WRKY蛋白质主要通过特异地结合靶基因启动子区域的W盒序列而调控其表达，但各家族成员的生物学功能存在着各自的特异性。通过生物信息学方法，结合大豆基因组数据库、高等植物基因组注释数据库和NCBI等相关数据库，识别、筛选并获得了大豆WRKY基因家族的蛋白序列，以实验室前期克隆得到大豆基因Glyma08g02580.1为目的基因，分析其在拟南芥、水稻等高等植物中的同源蛋白，并对其进行了系统进化分析；同时利用GEO的芯片数据对大豆在碱胁迫下的根和叶基因表达谱进行了分析，通过相关分析，获得了大豆基因组中全部的WRKY蛋白及对应的分类结果，由表达谱分析结果进一步证明了WRKY基因表达的组织特异性。
Abstract:
Genes containing the WRKY domain are plant-specific transcriptional regulators and are expressed in various developmental stages and tissues.The most archaic WRKY transcription factors,which may originate some 1.5 billion to 2 billion years ago in eukaryotes,contain two highly conserved WRKY domain.Although all WRKY proteins regulate mainly the expression of target genes by specifically binding to the(T)(T)GACC(A/T)(W box)sequences of their promoter,the biological function of each member is specific.In the study,a bioinformatics analysis on WRKY family genes in Glycine max was performed.Based on bioinformatics methods,taking advantage of soybean database combined with public database(NCBI，TIGR，TAIR)，196 WRKY protein from soybean genome and download gene expression profile from GEO database were identified.Meanwhile,197 homology protiens of Glyma08g02580.1 in other plants were identified,such as Arabidopsis and Oryza sativa.The phylogenetic of 197 WRKY family protein sequence and domain characteristics were analyzed.The results of the investigation could be definitely provide a significant foundation for further research on function analysis of Glycine max WRKY gene family.

参考文献/References:

［1］Chen L,Song Y,Li S,et al.The role of WRKY transcription facotors in plant abiotic stresses[J].Biochimica et Biophysica Acta,2012,1819(2):120-128.

[2]Eulgem T,Rushton P J,Schmelzer E,et al.Early nuclear events in plant defence signaling:Rapid gene activation by WRKY transcription factors[J].The EMBO Journal,1999,18:4689-4699.
[3]贾翠玲.葡萄WRKY基因超家族的进化分析[D].大连：辽宁师范大学,2010.(Jia C L.Phylogenitic analyses of vitis vinifera WRKY gene superfamily[D].Dalian:Liaoning Normal University,2010.)
[4]Ciolkowski I,Wanke D,Birkenbihl R P,et al.Studies on DNA-binding selectivity of WRKY transcription facotors lend structural clues into WRKY-domain function[J].Plant Molecular Biology,2008,68(1/2):81-92.
[5]Yokotani N,Sato Y,Tanabe S,et al.WRKY76 is a rice transcriptional repressor playing opposite roles in blast disease resistance and cold stress tolerance[J].Journal of Experimental Botany,2013,64(16):5085-5097.
[6]Lan A,Huang J,Zhao W,et al.A salicylic acid-induced rice(Oryza sativa L.)transcription factor OsWRKY77 is involved in disease resistance of Arabidopsis thaliana[J].Plant Biology,2013,15(3):452-461.
[7]Yu F,Huaxia Y,Lu W,et al.GhWRKY15,a member of the WRKY transcription factor family identified from cotton(Gossypium hirsutum L.),is involved in disease resistance and plant development[J].BMC Plant Biology,2012,12；12:144.
[8]Ulker B,Somssich I E.WRKY transcription factors:from DNA binding towards biological function[J].Current Opinion in Plant Biology,2004,7(5):491-498.
[9]宋钰,荆绍娟，余迪求.水稻WRKY转录调控因子基因功能研究进展[J].中国水稻科学，2009,23(5):447-455.(Song Y,Jing S J,Yu D Q.Research progress on function analysis of rice WRKY genes[J].Chinese Journal of Rice Science,2009,23(5):447-455.)
[10] Nakayama A,Fukushima S,Goto S,et al.Genome-wide identification of WRKY45-regulated genes that mediate benzothiadiazole-induced defense responses in rice[J].BMC Plant Biology,2013；13:150 doi:10.1186/1471-2229-13-150.
[11] Dong J,Chen C,Chen Z.Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response[J].Plant Molecular Biology,2002,51:21-37.
[12] Lan A,Huang J,Zhao W,et al.A salicylic acid-induced rice(Oryza sative L.)transcription factor OsWRKY77 in disease resistance of Arabidopsis thaliana[J].Plant Biology,2013,15(3):452-461.
[13] Hwang S H,Yie S W,Hwang D J.Heterologous expression of OsWRKY6 gene in Arabidopsis activates the expression of defense related genes and enhances resistance to pathogens[J].Plant Science,2011,181(3):316-323.
[14] Yoda H,Ogawa M,Yamaguchi Y,et al.Identification of early-responsive genes associated with the hypersensitive response to tobacco mosaic virus and characterization of a WRKY-type transcription factor in tobacco plants[J].Molecular Genet Genomics,2002,267(2):154-161.
[15] Mare C,Mazzucotelli E,Crosatti C,et al.Hv-WRKY38:A new transcription factor involved in cold and drought response in barley[J].Plant Molecular Biology,2004,55(3):399-416.
[16]田云，卢向阳，彭丽莎,等.植物WRKY转录因子机构特点及其生物学功能[J].遗传，2006,28(12):1607-1612.(Tian Y,Lu X Y,Peng L S,et al.The structure and function of plant WRKY transcription factors[J].Hereditas,2006,28(12):1607-1612.)
[17] Yu D Q,Chen L G,Zhang L P.Transcription factors WRKY superfamily:Origin,structure and function[J].Acta Botanica Yunnanica,2006,28(1):69-77.
[18] Yu D,Chen C,Chen Z.Evidence for an important role of WRKY DNA binding protein in the regulation of NPR1 gene expression[J].The Plant Cell,2001,13:1527-1539.
[19] Luo X,Bai X,Zhu D,et al.Expression of wild soybean WRKY20 in Arabidopsis enhances drought tolerance and regulates ABA signalling[J].Journal of Experimental Botany,2013,64(8)：2155-2169.
[20] Yamasaki K,Kigawa T,Seki M,et al.DNA-binding domains of plant-specific transcription facotors:structure,function,and evolution[J].Trends Plant Science,2013,18(5):267-276.
[21] Cheng Y,Zhou Y,Yang Y,et al.Structural and functional analysis of VQ motif-containing proteins in Arabdopsis as interacting proteins of WRKY transcription factors[J].Plant Physiology,2012,159(2):810-825.
[22]李蕾，谢丙炎，戴小枫，等.WRKY转录因子及其在植物防御反应中的作用［J］.分子植物育种，2005，3（3）：401-408.(Li L,Xie B Y,Dai X F,et al.WRKY transcription factors and their roles in plant defense response[J].Molecular Plant Breeding,2005，3(3):401-408）.
[23]张颖，蒋卫杰，凌键，等.WRKY转录因子表达谱的研究进展[J].基因组学与应用生物学，2009,28（4)：803-808.(Zhang Y,Jiang W J,Ling J,et al.Advance on expression profile of transcription factor WRKY[J].Genomics and Applied Biology,2009,28(4):803-808.)
[24]Xiong W,Xu X,Zhang L,et al.Genome-wide analysis of the WRKY gene family in physic nut(Jatropha curcas L.)[J].Gene,2013,524(2):124-132.

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备注/Memo

基金项目：教育部高等学校博士学科点专项科研基金（20102325120002)；国家自然科学基金（31371544)；教育部科学技术研究重点项目（212049)。

第一作者简介：刘冬冬（1986-)，男，硕士，主要从事植物分子遗传学研究。E-mail:liudongdong0524521@hotmail.com。
通讯作者：柏锡（1975-)，男，博士，副教授，主要从事植物分子生物学与逆境生理学研究。E-mail:baixi@neau.edu.cn。

更新日期/Last Update: 2014-12-24