[1]刘德泉,郭文云,何则铭,等.大豆胚发育期酵母双杂文库的构建及与bHLH转录因子互作蛋白的筛选[J].大豆科学,2015,34(05):789-793.[doi:10.11861/j.issn.1000-9841.2015.05.0789]
　LIU De-quan,GUO Wen-yun,HE Ze-ming,et al.Yeast Two-hybrid cDNA Library Construction of Soybean Embryo Developmental Phase and Screening of Proteins Interacting with Soybean bHLH Transcription Factor[J].Soybean Science,2015,34(05):789-793.[doi:10.11861/j.issn.1000-9841.2015.05.0789]
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大豆胚发育期酵母双杂文库的构建及与bHLH转录因子互作蛋白的筛选

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年05期 页码: 789-793 栏目: 出版日期: 2015-10-25
Title:
Yeast Two-hybrid cDNA Library Construction of Soybean Embryo Developmental Phase and Screening of Proteins Interacting with Soybean bHLH Transcription Factor
作者:
刘德泉; 郭文云; 何则铭; 苏连泰; 李景文; 王英; 张海军; 王庆钰
吉林大学 植物科学学院，吉林 长春 130062
Author(s):
LIU De-quan;  GUO Wen-yun;  HE Ze-ming;  SU Lian-tai;  LI Jing-wen;  WANG Ying;  ZHANG Hai-jun; WANG Qing-yu
College of Plant Science, Jilin University, Changchun 130062, China
关键词:
大豆; 发育; 酵母双杂; 转录因子; cDNA文库
Keywords:
Soybean;  Development;  Yeast two-hybrid;  Transcription factors;  cDNA library
DOI:
10.11861/j.issn.1000-9841.2015.05.0789
文献标志码:
A
摘要:
构建了大豆幼胚生长期20~50 d DSN(duplex-specific nulease)均一化cDNA酵母双杂文库，总克隆数为2.8×107 cfu。为了筛选能与已知大豆bHLH转录因子GmbHLH3a相互结合的蛋白质，以转录因子GmbHLH3a为诱饵蛋白构建诱饵载体，GmbHLH3a诱饵载体无自激活活性，通过酵母双杂筛选到了两个能与诱饵蛋白相互作用的蛋白及其序列。
Abstract:
In present study, DSN (duplex-specific nulease)-normalization yeast two-hybrid cDNA libraries of soybean seed development from 20 to 50 days after flowering were constructed.The total clone number was 2.8×107cfu. In order to obtain the proteins that could bind with the known soybean bHLH transcription factor GmbHLH3a,the bait plasmid of GmbHLH3a, whose carrier has no auto-activation, was constructed. Through yeast two-hybrid screening, two proteins which could interact with the bait plasmid and their corresponding sequences were acquired.

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备注/Memo

基金项目： “十二五”农村领域国家科技计划子任务(2011BAD35B06-2)；国家转基因生物新品种培育科技重大专项（2014ZX08004-003）；国家自然基金面上项目（31371641）。

第一作者简介：刘德泉（1988-），男，硕士，主要从事大豆分子育种研究。E-mail：dequanliu1988@qq.com。
通讯作者：王庆钰（1963-），女，博士，教授，主要从事基因工程在育种上的应用研究。E-mail：qywang@jlu.edu.cn。

更新日期/Last Update: 2015-11-07