[1]孙晶,盛碧涵,韩英鹏,等.大豆基因组WRKY31同源基因的生物信息学分析[J].大豆科学,2014,33(05):642-647.[doi:10.11861/j.issn.1000-9841.2014.05.0642]
　SUN Jing,SHENG Bi-han,HAN Ying-peng,et al.Bioinformatics Analysis of WRKY31 Homologs in Soybean Genome[J].Soybean Science,2014,33(05):642-647.[doi:10.11861/j.issn.1000-9841.2014.05.0642]
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大豆基因组WRKY31同源基因的生物信息学分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年05期 页码: 642-647 栏目: 出版日期: 2014-10-25
Title:
Bioinformatics Analysis of WRKY31 Homologs in Soybean Genome
文章编号:
1000-9841.2014.05.0642
作者:
孙晶; 盛碧涵; 韩英鹏; 赵雪; 王强; 孟宪新; 魏淑红; 李文滨
东北农业大学大豆生物学教育部重点实验室，东北农业大学农业部东北大豆生物学与遗传育种重点实验室，黑龙江 哈尔滨 150030
Author(s):
SUN Jing; SHENG Bi-han; HAN Ying-peng; ZHAO Xue; WANG Qiang; MENG Xian-xin; WEI Shu-hong; LI Wen-bin
Key Laboratory of Soybean Biology in Chinese Ministry of Education,Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry,Northeast Agricultural University,Harbin 150030,China
关键词:
大豆; WRKY31; 胁迫; WRKY结构域; 系统进化
Keywords:
Soybean; WRKY31; Stress; WRKY domain; Phylogenetic
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0642
文献标志码:
A
摘要:
WRKY转录因子是一类参与多种生物和非生物胁迫的诱导性转录调控因子，在植物进化上高度保守。通过克隆大豆WRKY31基因全长和生物信息学分析，发现WRKY31在大豆基因组中存在3个同源基因，分别位于第6、12和13号染色体上；WRKY31蛋白含有1个WRKY结构域（343～419 aa）和C2H2型锌指结构，属于第二类WRKY基因。RT-PCR分析表明，GmWRKY31在大豆的叶、花、豆荚、根、根瘤中的组织内均有表达，且在花中表达量最高，豆荚中最低。通过MEGA 5.0软件分析，大豆和芸豆WRKY蛋白亲缘关系更近，位于同一分支上，而它的2个拷贝Glyma06g46420.2和Glyma12g10350.1位于另一个分支上，亲缘关系较近。蛋白结构及功能预测表明WRKY31是一类转录因子，参与胁迫反应；系统进化分析表明其基因在进化过程中功能上发生了重大分化。
Abstract:
The gene family of WRKY is transcription factor that can be induced and involved in the response to biotic and abiotic stresses,and is conserved evolutionarily.In this research,the full-length cDNA of GmWRKY31 was cloned and bioinformatics analysis was used to predict the potential function of GmWRKY31.Phylogenetic analysis of GmWRKY31 and its homologs with different plant species were conducted by the software MEGA 5.0.The results showed that three copies of WRKY31 exited in the soybean genome,located on the chromosome 6,12 and 13.GmWRKY31 belongs to the type II WRKY transcription factors,with a WRKY domain and a C2H2-type zinc finger.Real-time RT-PCR analysis showed that GmWRKY31 was expressed in soybean leaf,flower,pod,root,nodule,and the flower had the highest expression,the lowest expression was in the pod.The result of the phylogenetic tree indicted that WRKY31 from Glycine max and Phaseolus vulgaris were the nearest in genetic relationship.While Glyma06g46420.2 and Glyma12g10350.1(the other two copies of WRKY31 of Glycine max) had closer relationship.The result of protein structure and function prediction showed that GmWRKY31 was a transcription factor being involved in the stress response of plant,however,the phylogenetic tree indicted that significant difference of the WRKY genes happened in evolution of soybean.

参考文献/References:

［1］Guilfoyle T J.The structure of plant gene promoters[J].Genetic Engineering,1997,19:15-47.

[2]Ishiguro S,Nakamura K.Characterization of a cDNA encoding a novel DNA-binding protein,SPF1,that recognizes SP8 sequences in the 5’upstream regions of genes coding for sporamin and beta-amylase from sweet potato[J].Molecular and General Genetics,1994,244(6):563-571.
[3]Rushton P J,Somssich I E,Ringler P,et al.WRKY transcription factors[J].Trends in Plant Science,2010,15(5):247-258.
[4]Schmutz J,Cannon S B,Schlueter J,et al.Genome sequence of the palaeopolyploid soybean[J].Nature,2010,463(7278):178-183.
[5]Song Y,Ai C R,Jing S J,et al.Research progress on functional analysis of rice WRKY genes[J].Rice Science,2010,17(1):60-72.
[6]Zhang Y J,Wang L J.The WRKY transcription factor superfamily:its origin in eukaryotes and expansion in plants[J].BMC Evolutionary Biology,2005,5:1-12.
[7]Clive J.Global status of commercialized biotech/GM crops:2008［M］.New York:ISAAA,2008:4-5.
[8]Michael I P,Monetti C,Chiu A C,et al.Highly efficient site-specific transgenesis in cancer cell lines[J].Molecular Cancer,2012,11(1):89-99.
[9]Eulgem T,Rushton P J,Robatzek S,et al.The WRKY superfamily of plant transcription factors[J].Trends in Plant Science,2000,5(5):199-206.
[10]Maleck K,Levine A,Eulgem T,et al.The transcriptome of Arabidopsis thaliana during systemic acquired resistance[J].Nature Genetics,2000,26(4):403-410.
[11]Ulker B,Somssich I E. WRKY transcription factors:from DNA binding towards biological function[J].Current Opinion in Plant Biology,2004,7(5):491-498.
[12]王春梅.浅谈大豆高产病虫害的防治方法[J].科技致富向导,2014(8):11.(Wang C M.Discussion on prevention and treatment of diseases and insect pests of soybean yield[J].The Wizard Rich Technology,2014(8):11.)
[13]Xie Z,Zhang Z L,Zou X L,et al.Interactions of two abscisic-acid induced WRKY genes in repressing gibberellin signaling in aleurone cells[J].The Plant Journal,2006,46 (2):231-242.
[14]Zou X L,Neuman D,Shen Q J.Interactions of two transcriptional repressors and two transcriptional activators in modulating gibberellin signaling in aleurone cells[J].Plant Physiology,2008,148 (1):176-186.
[15]Ramamoorthy R,Jiang S Y,Kumar N,et al.A comprehensive transcriptional profiling of the WRKY gene family in rice under various abiotic and phytohormone treatments[J].Plant Cell Physiology,2008,49(6):865-879.
[16]Dong J,Chen C,Chen Z.Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response[J].Plant Molecular Biology,2003,51(1):21-37.
[17] Liu X,Bai X,Wang X,et al.OsWRKY71,a rice transcription factor,is involved in rice defense response[J].Journal of Plant Physiology,2007,164:969-979.
[18]仇玉萍,荆邵娟,付坚,等.13个水稻WRKY基因的克隆及其表达谱分析[J].科学通报,2004,49(18):1860-1869.(Qiu Y P,Jing S J,Fu J,et al.Clone and expression profiling analysis of 13 WRKY transcription factorsin rice[J].Chinese Science Bulletin,2004,49 (18):1860-1869.)
[19] Marè C,Mazzucotelli E,Crosatti C,et al.Hv-WRKY38:a new transcription factor involved in cold- and drought-response in barley[J].Plant Molecular Biology,2004,55:399-416.
[20]Seki M,Ishida J,Nanjo T,et al.Monitoring the expression profiles of 7000 Arabidopsis genes under drought,cold and high-salinity esses using a full-length cDNA microarray[J].The Plant Journal,2002,31(3):279-292.

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备注/Memo

基金项目：国家“十二五”科技支撑计划（2011BAD35B06-1）；现代农业产业技术体系（CARS-04-PS04）；国家重点基础研究发展计划“973计划”前期项目（2012CB126311）；国家自然基金（31201227，31301339）；中国博士后项目（20110491024）；黑龙江省博士后项目(LBH11220，LBH-TZ1210)；黑龙江省教育厅科学技术研究项目(12541049)；黑龙江省教育厅骨干教师项目（1252G014）；黑龙江省教育厅新世纪项目优秀人才资助项目（1253-NCET-005）；教育部博士点项目（20122325120012）；东北农业大学博士后启动金项目（2012RCB11）；大豆生物学教育部重点实验室开放基金项目（SB12A05）。

第一作者简介：孙晶（1990-），女，硕士，主要从事大豆遗传育种研究。E-mail:sj6238434@163.com。
通讯作者：李文滨（1958-），男，教授，博导，主要从事大豆遗传育种研究。E-mail:wenbinli@neau.edu.cn。

更新日期/Last Update: 2014-12-24