SUN Lei,HAN Jun-you,LI Hong-yu.Comparison on Methods Screening of Transgenic Soybean Resistant to Glufosinate[J].Soybean Science,2010,29(06):1019-1023.[doi:10.11861/j.issn.1000-9841.2010.06.1019]
大豆再生植株抗草铵膦的筛选方法研究
- Title:
- Comparison on Methods Screening of Transgenic Soybean Resistant to Glufosinate
- 文章编号:
- 1000-9841（2010）06-1019-05
- Keywords:
- Soybean; Regeneration; Screening; Glufosinate
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 为解决农杆菌介导的大豆子叶节转化试验中草铵膦筛选所得转基因后代假阳性高的问题，采用浸种法、根吸法、叶片涂抹法、叶片喷洒法4种筛选方法和多种筛选浓度对大豆再生植株及其后代抗性进行筛选。结果采用1∶200 草铵膦浓度的叶片喷洒法效果最佳。叶片喷洒法筛选6802株转化植株，获得了9株抗性植株，其后代的分子检测表明均为阳性植株。因此，采用叶片喷洒法对大豆抗草铵膦植株进行筛选的方法简便可行。
- Abstract:
- Four screening methods and several screening concentrations were used to screen soybean regeneration plant and the resistance of its progeny as well as to resolve the problem of high false-positive regeneration plants in agrobacterium-mediated transformation of soybean cotyledons after glufosinate screening. After screening the 6802 transgenic plants by spraying the leaf, 9 resistant plants were obtained, and the molecular detection of their progeny showed that they were all positive transgenic plants. Therefore, the results indicated that the method that screening the transgenic plant by the leaf spraying was feasible.
参考文献/References:
[1]余永亮， 梁慧珍， 王树峰， 等.中国转基因大豆的研究进展及其产业化[J].大豆科学，2010，29(1)：143-150. （Yu Y L, Liang H Z, Wang S F, et al. Research progress and commercialization on transgenic soybean in China[J].Soybean Science，2010，29(1)：143-150.） [2]盛东峰， 杨同文. 转基因大豆的研究及应用[J].周口师范学院学报，2007，25(2)：83-85.（Sheng D F，Yang T W. Transgenic soybean research and security assessment[J].Journal of Zhoukou Normal University, 2007，25(2)：83-85.） [3]Vain P, McMullen M D, Finer J J. Osmotic treatment enhances particle bombardment-mediated transient and stable transformation of maize[J].Plant Cell Reprots,1993,12:84-88. [4]Chen S Y. High-efficiency agrobacterium-mediated transformation of soybean [J]. Acta Botanica Sinica, 2004, 46(5):610-617. [5]薛仁镐,谢宏峰. 利用改良的草铵膦筛选系统快速而有效筛选转基因大豆[J]. 大豆科学，2006, 25(4):373-378.(Xue R G, Xie H F. Rapid and efficient selection for transgenic soybean plants with the improved glufosinate selection system[J].Soybean Science, 2006, 25(4):373-378.) [6]唐晓飞, 刘丽君, 张小明, 等. 高产大豆新品系哈交5337和哈交5489再生条件的优化[J]. 大豆科学，2008，27(4):203-207. （Tang X F, Liu L J, Zhang X M, et al. Improvement of regeneration system in high yield soybean lines Hajiao5337 and Hajiao5489[J]. Soybean Science，2008, 27(4):203-207.） [7]寇坤， 刘丽君， 曲姗姗， 等.大豆新品系黑农56子叶节再生体系的优化[J].大豆科学，2009，28(3)：400-408.（Kou K，Liu L J, Zhang X M, et al. Improvement of regeneration system in new soybean line Heinong 56[J]. Soybean Science，2009, 28(3):400-408.） [8]刘敏， 朱洪德， 高中利.抗除草剂转基因大豆遗传分析[J].大豆科学，2010，29(1)：33-36.（Liu M, Zhu H D,Gao Z L.Inheritance analysis of herbicide-resistant transgenic soybean[J].Soybean Science，2010，29(1)：33-36.） [9]刘晓冰， Stephen J．Herbert, 金剑, 等.增加光照及其与改变源库互作对大豆产量构成因素的影响[J].大豆科学，2006，25(1)：6-10.（Liu X B，Herbert S J，Jin J, et al. Light enrichement and its interactions with source-sink alteration on yield and yield components in soybean[J]. Soybean Science,2006,25(1):6-10.） [10]应珊， 何晓薇， 王秀荣， 等.影响农杆菌介导的大豆转化效率的因素研究[J].分子植物育种，2008，6(1)：32-40.（Ying S，He X W，Wang X R，et al. Assessment of factors affecting the transformation efficiency of soybean cotyledonary-node agrobacterium-mediated transformation system[J].Molecular Plant Breeding,2008,6(1):32-40.） [11]宋雯雯, 刘宝辉, 杨明亮, 等. 农杆菌介导法将SPS基因导入大豆的研究[J].大豆科学，2008，27(3):387-390. （Song W W，Liu B H, Yang M L, et al. Transformation of SPS gene into soybean via agrobacterium-mediated method[J]. Soybean Science, 2008，27(3):387-390.） [12]王庆中. 转BoTMT基因大豆的获得及鉴定[D].北京：中国农业科学院，2006：20-23. （Wang Q Z. Obtaining and identification of transgenic soybean with the gene of BoTMT[D]. Beijing: Chinese Academy of Agricultural Sciences,2006:20-23.）
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(06):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(06):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(06):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(06):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(06):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(06):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(06):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(06):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(06):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(06):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]王晓春,刘尚前王 罡季 静王 萍.大豆体细胞胚再生植株的研究＊[J].大豆科学,2006,25(02):149.[doi:10.11861/j.issn.1000-9841.2006.02.0149]
Wang Xiaochun Liu Shangqian Wang Gang Ji Jing Wang Ping.A STUDY OF SOYBEAN PLANT REGENERATION VIA SOMATIC TISSUE CULTURES[J].Soybean Science,2006,25(06):149.[doi:10.11861/j.issn.1000-9841.2006.02.0149]
[12]叶兴国,王连铮.大豆花药培养研究进展[J].大豆科学,2018,37(王连铮先生专辑):153.[doi:10.11861/j.issn.1000-9841.1995.04.0349]
[13]周思君,尹光初,雷勃钧,等.从大豆幼胚诱导器官发生再生植株[J].大豆科学,1990,9(04):285.[doi:10.11861/j.issn.1000-9841.1990.04.0285]
[J].Soybean Science,1990,9(06):285.[doi:10.11861/j.issn.1000-9841.1990.04.0285]
备注/Memo
基金项目：国家高技术研究发展计划资助项目（2006AA10Z107）；转基因专项资助项目（2008ZX08010-004，2009ZX08004-010B）。