[1]沈文飚徐朗莱冯晴高玲胡蕴珠孙军礼智海剑.大豆抗SMV 生化机制的初步研究[J].大豆科学,1999,18(01):22-26.[doi:10.11861/j.issn.1000-9841.1999.01.0022]
　Shen WenbiaoXu Lang laiFeng QingGao LingHu YunzhuSun JunliZhi Hai jian.A PRELIMINARY STUDY ON BIOCHEMICALMECHANISM OFRESISTANCE TO SMV IN SOYBEAN[J].Soybean Science,1999,18(01):22-26.[doi:10.11861/j.issn.1000-9841.1999.01.0022]
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大豆抗SMV 生化机制的初步研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第18卷 期数: 1999年01期 页码: 22-26 栏目: 出版日期: 1999-02-25
Title:
A PRELIMINARY STUDY ON BIOCHEMICALMECHANISM OFRESISTANCE TO SMV IN SOYBEAN
作者:
沈文飚1徐朗莱1冯晴2高玲1胡蕴珠3孙军礼3智海剑3
1南京农业大学理学院生化室　南京210095　
2上海师范大学生物系,上海200234　
3南京农业大学大豆研究所　南京210095
Author(s):
Shen Wenbiao　Xu Lang lai　Feng Qing　Gao LingHu Yunzhu　Sun Junli　Zhi Hai jian
College of Sciences, N anjing Agricultural University , Nanjing , 210095
关键词:
大豆;  大豆花叶病毒;  生化抗性机制
Keywords:
Soy bean;  Soy bean mosaic vi rus;  Biochemical resistant mechani sm
DOI:
10.11861/j.issn.1000-9841.1999.01.0022
文献标志码:
A
摘要:
以对SMV - a 株系抗性不同的3个大豆品种为材料,分析了感染SMV 后苯丙氨酸解氨酶( PAL)、过氧化物酶( POD)、超氧化物歧化酶( SOD)和过氧化氢酶( CAT)活性的动态变化。结果表明,抗病品种科丰1号接种SMV 的早期, PAL和POD活性显著升高, SOD和CAT活性明显下降; 相反感病品种1138- 2和徐豆1号的SOD和CAT活性明显上升, POD活性的增加幅度则小于抗病品种,而1138-2的PAL活性则低于相应对照。
Abstract:
Dynamic chang es in activi ties o f phenylalanine ammo nia lyase ( PAL) , pero xida se( POD) , superox ide dismutase ( SOD) and catalase ( CAT) w ere tested wi th leav es of resistant a nd susceptible soy bean cultiv ars during infectio n by SMV - a. The resul ts suggested that a t the early stag e of infection PAL a nd POD activi ties o f resista nt so ybeancultiva r ( Kefeng- 1) increased rapidly , SOD and CA T activ ities decreased dramatically.On the o ther hand, the chang es o f PO D w ere relativ ely smal ler in leav es of susceptiblesoybean cultiv ars ( 1138- 2 a nd Xudo u- 1) , SOD and CAT activ ities show ed an increaseand PAL activ ities o f 1138- 2 declined.

参考文献/References:

[1 ]　庄炳昌、徐豹、廖林, 1993,接种大豆花叶病毒后,大豆叶片超氧化物歧化酶、过氧化物酶和蛋白组份的变化,植物病理学报, 23( 3): 261- 265

[2 ]　徐朗莱、叶茂炳, 1989,过氧化物酶活力连续记录测定法,南京农业大学学报, 12( 3): 80- 83
[3 ]　沈文飚、叶茂炳、徐朗莱等, 1997,小麦旗叶自然衰老过程中清除活性氧能力的变化,植物学报, 39( 7): 634-640
[4 ]　欧阳光察、薛应龙, 1988,植物苯丙烷类代谢的生理意义及其调控, 植物生理学通讯, 24( 3): 9- 16
[5 ]　沈文飚、徐朗莱、胡蕴珠等, 1997,大豆花叶病毒( SMV)的侵染对大豆叶片过氧化物酶及其同功酶的影响,南京农业大学学报, 20( 4): 88- 92
[6 ]　Bradf ord, M. M. , 1976, A rapid and sensi tive meth od f or th e quanti tat ion of microg ram quanti ties of proteinuti li zing the p rincipl e of prot ein - dye binding, Anal Bioch em, 72: 248- 254
[7 ]　Koukol, J. , E. E. Conn, 1961, The met aboli sm of aromatic compounds in high er plant s. IV. Pu rif icat ion,J.Biol. Ch em, 236: 2692- 2698
[8 ]　Meh dy M. C. , 1994, Activ e oxyg en species in plant def ens e agains t pathogens , Plant Physi ol , 105: 467-472

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更新日期/Last Update: 2016-12-26