大豆盐胁迫响应NRT基因的鉴定及GmNRT1.5A的克隆和表达分析
- Title:
- Identification of Soybean Salt Stress Responsive NRT Genes and Cloning and Expression Analysis of GmNRT1. 5A
- 关键词:
- 大豆; 盐胁迫; NRTs; GmNRT1. 5A; 表达分析
- 摘要:
- 硝酸盐转运蛋白（Nitrate Transporters，NRTs）在植物根系NO3-吸收或转运中发挥重要作用，为探究大豆NRTs基因在盐胁迫响应中的功能，本研究从前期盐胁迫处理的转录组数据中鉴定出8个持续响应盐胁迫的差异表达GmNRTs基因。这些GmNRTs基因的启动子上均含有多个与逆境胁迫或植物激素应答相关的元件，推测它们可能与大豆非生物胁迫应答或植物激素调控盐胁迫响应相关。同时，从齐黄34根系中克隆了盐胁迫处理上调倍数最大的基因GmNRT1.5A，并对其进行了生物信息学和表达分析。结果显示：GmNRT1.5A的蛋白质编码区全长为1 794 bp，编码597个氨基酸，蛋白分子质量为66.78 kD，理论等电点为5.85。进化树分析结果表明，GmNRT1.5A与AtNRT1.5同源性最高，含有12个跨膜结构域，含有典型的NRT1s保守结构域，二级结构以α螺旋和无规则卷曲为主。实时荧光定量PCR分析表明，GmNRT1.5A主要在籽粒和根系中表达，盐胁迫和干旱胁迫显著诱导根系中GmNRT1.5A的表达。此外，GmNRT1.5A的表达受植物激素的调控，ABA和ACC显著诱导其表达，但GA3处理显著抑制GmNRT1.5A表达。本研究表明，GmNRT1.5在响应盐胁迫逆境中发挥重要作用，为探索GmNRTs基因在响应逆境胁迫中的功能提供了新思路，也为深入研究GmNRT1.5基因的功能提供了基础。
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