[1]刘德泉,聂波涛,邱红梅,等.野生大豆×栽培大豆RIL群体高密度遗传图谱构建及SNP偏分离分析[J].大豆科学,2023,42(06):641-652.[doi:10.11861/j.issn.1000-9841.2023.06.0641]
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野生大豆×栽培大豆RIL群体高密度遗传图谱构建及SNP偏分离分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第42卷 期数: 2023年06期 页码: 641-652 栏目: 出版日期: 2023-11-20
Title:
High Density Genetic Map Construction and SNP Segregation Distortion Analysis in RIL Population of Wild Soybean × Cultivated Soybean
作者:
刘德泉1; 聂波涛1; 2; 邱红梅1; 陈 亮1; 陈 健1; 崔正果1; 姬文秀2; 王跃强1
1.吉林省农业科学院 大豆研究所，吉林 长春 130033；2.延边大学 农学院，吉林 延吉 133002
关键词:
栽培大豆; 野生大豆; 偏分离; SNP; 重组自交系
DOI:
10.11861/j.issn.1000-9841.2023.06.0641
摘要:
通过研究野生大豆与栽培种大豆群体构建过程中产生的偏分离现象，发掘偏分离区间（Segregation Distortion Region，SDR）和候选基因，有助于探究偏分离在大豆中的产生机制。应用地方品种“一千粒”和野生品种“长岭野生豆”配置杂交组合，获得F6代重组自交系（Recombinant Inbred Lines，RIL）株系200株，利用SLAFseq进行测序分析，构建高密度遗传图谱，获得该群体可靠的4 564个SNP标记。偏分离分析发现，648个标记发生偏分离（P<0.05），占总标记的14.20%。获得22个SDR，分布在9个不同的染色体上。在SDR区间内共发现8个重度偏分离热点区域（Extreme Segregation Distortion，ESDR），分布在5个不同的染色体上，其中3个ESDR偏向父本野生型，5个ESDR偏向母本栽培型。利用基因功能注释及全基因组重测序数据，结合ESDR区域，影响胚胎发育（Glyma.01G051400）及雌配子体发育（Glyma.16G072700）的基因分别被认为是ESDR11和ESDR161的候选基因。本研究结果为今后偏分离基因定位提供可靠依据，并为阐明偏分离现象奠定基础。

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更新日期/Last Update: 1900-01-01