LI Fa-yuan,TIAN Fang,ZHANG Xiao-ke,et al.Analysis of Seedlings Salt Tolerance of Backcross Hybrids of Glycine maxand Glycine soja[J].Soybean Science,2012,31(04):593-597.[doi:10.3969/j.issn.1000-9841.2012.04.016]
栽培大豆和野生大豆及其回交后代苗期耐盐性分析
- Title:
- Analysis of Seedlings Salt Tolerance of Backcross Hybrids of Glycine maxand Glycine soja
- 文章编号:
- 1000-9841（2012）04-0593-05
- Keywords:
- Glycine max; Glycine soja; Backcross hybrids; Salt tolerance
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以逐代人工耐盐性筛选的栽培和野生大豆杂交组合（Lee68×N23227）F5代株系3060为父本，以耐盐栽培大豆Lee68品种为母本或轮回亲本，配置回交组合（Lee68×3060,编号C）得到的不同单株后代株系（BC1F2）为试验材料，采用苗期耐盐系数、相对生长速率、干物质积累量等指标，对它们的耐盐性进行了分析和评价，并采用相对电解质渗漏率、硫代巴比妥酸反应物（TBARS）含量、叶片净光合速率（Pn）和PSⅡ最大光化学效率（Fv/Fm）对其中优良株系耐盐的生理机制进行了分析和探讨。结果表明：在盐胁迫下，该组合回交后代中C3、C10等株系的苗期耐盐性系数、相对生长速率和干物质积累量等指标均明显高于母本Lee68，接近或超过父本3060，其中C10株系尤为突出。与双亲相比，盐胁迫使C10株系幼苗根和叶中相对电解质渗漏率和TBARS含量的上升幅度，及叶片Pn和Fv/Fm值的降低幅度明显较低，且盐胁迫下的相对电解质渗漏率和TBARS含量与其对照相比，差异均未达显著水平。可认为C10株系是一种新的优良耐盐大豆种质材料。
- Abstract:
- In this study,the hybrid strain(F5)3060 selected for salt tolerance generation by generation from the cross combination (Glycine max,Lee68 cultivar × Glycine soja,N23227 population)was used as the male parent,the Lee68 was used as the female parent or recurrent parent,the backcross combination（Lee68×3060,named as C）was configured,and several single hybrid strains（BC1F2）were obtained.Using these soybean materials,their salt tolerance was evaluated with parameters including the salt tolerance coefficient,relative growth rate and dry matter accumulation.On that basis,the salt tolerance mechanism of one fine strain was investigated with parameters,such as the relative electrolytic leakage,thiobarbituric acid reactive substances（TBARS）content,Pn and the maximum photochemical efficiency of photosystem Ⅱ(Fv/Fm).The results showed that,under salt stress,the salt tolerance coefficient,relative growth rate and dry matter accumulation in C3 and C10 hybrid strains were obviously higher than those in their female parent Lee68,and almost be equal to or more than those in the male parent 3060 strain,especially the C10 strain displayed the most.When compared with their two parents,the increase of the relative electrolytic leakage and TBARS content in roots and leaves,and the decrease of Pn and Fv/Fm in leaves of C10 seedlings under salt stress were distinctly lower.Moreover,the relative electrolytic leakage and TBARS content in roots and leaves of C10 seedlings under salt stress were not significantly different compared to its control.So,the C10 hybrid strain could be considered as one excellent salt tolerant soybean germplasms for future study.
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备注/Memo
国家自然科学基金项目（30871462）；国家转基因生物新品种培育重大专项（2009ZX08004-008B）。