TIAN Xin-yue,HU Ying-lu,WANG Zi-jie,et al.Effects of Soybean Organs in Vitro Feeding on the Growth and Development of Riptortus Pedestris Nymphs[J].Soybean Science,2021,40(03):398-402.[doi:10.11861/j.issn.1000-9841.2021.03.0398]
大豆离体器官饲养对点蜂缘蝽若虫生长发育的影响
- Title:
- Effects of Soybean Organs in Vitro Feeding on the Growth and Development of Riptortus Pedestris Nymphs
- Keywords:
- Soybean; Riptortus pedestris; Nymphs organ in vitro; Developmental period; Nutritional composition
- 文献标志码:
- A
- 摘要:
- 为明确大豆植株离体器官饲养对点蜂缘蝽若虫（Riptortus pedestris）生长发育的影响，研究点蜂缘蝽为害大豆的主要器官，采用大豆离体叶、茎、花、荚饲养点蜂缘蝽，系统观测其若虫生长发育情况，测定这些器官的脂肪、总蛋白和总糖等主要营养成分含量，并分析营养成分含量与存活率间的关系。结果表明：在24±1 ℃恒温下，以离体荚饲养的若虫可顺利完成个体发育，种群存活曲线符合Ⅰ型曲线；以离体叶、茎、花饲养的若虫不能完成个体发育，种群存活曲线符合Ⅲ型曲线。荚的脂肪和总蛋白含量显著高于叶、茎、花。以叶、茎、花、荚饲养的点蜂缘蝽若虫期存活率与总蛋白、脂肪含量之间存在高度正相关性，但与总糖含量之间无相关性。因此，大豆荚有利于点蜂缘蝽若虫存活，是其个体发育不可缺少的营养来源，也是其刺吸为害的主要器官；大豆荚中的脂肪和蛋白质是点蜂缘蝽若虫，特别是2~5龄若虫的主要营养成分。
- Abstract:
- In order to understand the effects of soybean plants organs in vitro feeding on the growth and development of Riptortus pedestris nymphs, study the main organs of soybean domaged by R.pedestris. The nymphs were fed with soybean leaves, stem, flowers and pods in vitro, respectively. The growth and development of the nymphs were systematically observed and the contents of main nutrient (fat, total protein and total sugar) were determined, and the relationship between nutrient contend and survival rate was analyzed. The results showed that the nymphs completed their individual development and growth successfully when fed with pods in vitro and their survival curve of the population was in accordance with type I, under 24±1 ℃ constant temperature. However, the nymphs did not completed their individual development and growth when fed with leaves, stem, and flowers in vitro, and their survival curve of the population was in accordance with type Ⅲ. The content of fat and protein in pod was significantly higher than that in leaf, stem and flower. There was a high positive correlation between the nymphs survival rate and the contents of total protein and fat, but there was no correlation between the nymphs survival rate and total sugar content. Therefore, soybean pod was beneficial to the survival of R. pedestris nymphs, which was an indispensable nutrition source for their individual development and growth and was also the main organ for their piercing and sucking. The fat and protein in soybean pod were the main nutrition composition for nymphs, especially for the 2nd to 5th instar nymphs.
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备注/Memo
收稿日期：2020-12-14