LIU Jia-lin,CHEN Qi,XIE Hui-min,et al.Genetic Structure and Diversity of Soybean Cultivars Released from Huang-Huai-Hai and Southern China Based on TRAP Marker[J].Soybean Science,2020,39(03):341-351.[doi:10.11861/j.issn.1000-9841.2020.03.0341]
黄淮海和南方大豆育成品种TRAP标记的遗传结构研究
- Title:
- Genetic Structure and Diversity of Soybean Cultivars Released from Huang-Huai-Hai and Southern China Based on TRAP Marker
- Keywords:
- Soybean; Huang-Huai-Hai; Southern China; Cultivar; TRAP marker; Gene diversity
- 文献标志码:
- A
- 摘要:
- 为采用新型分子标记技术有效评估与利用黄淮海和南方大豆种质资源，本研究通过目标区域扩增多态性（TRAP）功能标记对来自中国黄淮海和南方地域的158份大豆育成品种进行遗传结构及多样性分析。从随机组合的84组引物中筛选出21组多态性丰富的引物，共扩增出436条DNA条带，各引物条带数变幅为18～26个，平均20.7个。Nei′s基因多样性（H）变化范围为0.172 5～0.473 6，香农信息指数（I）变化范围为0.492 2～0.679 2，多态信息含量（PIC）变化范围为0.144 6～0.360 7。基于TRAP分子标记的聚类分析表明大豆材料共分为3类，其中I、II两小类亚群主要为黄淮海地域品种，III类大亚群黄淮海和南方地域品种分布均匀。Structure遗传结构分析将大豆育成品种划分为3个不同血缘关系。大豆材料的TRAP标记聚类分析和遗传结构分析结果表示大豆品种的分布无明显的地域相关性。
- Abstract:
- In order to evaluate and utilize the soybean germplasm resources in the Huang-Huai-Hai and southern region of China effectively with the new molecular marker technique, this research used target region amplification polymorphism (TRAP) to detect the genetic structure and diversity of 158 cultivated soybean from Huang-Huai-Hai and southern region of China. 21 groups of primers with rich polymorphism were selected from 84 groups of primers, which amplified 436 DNA bands totally. Each primer’s bands ranging from 18 to 26, and the average was 20.7. The range of Nei′s gene diversity (H) was 0.172 5-0.473 6, the Shannon information index (I) was 0.492 2-0.679 2, and the polymorphism information content (PIC) was 0.144 6-0.360 7. According to the cluster analysis based on TRAP molecular markers showed that soybean materials were divided into three categories, of which the two subgroups I and II were mainly Huang-Huai-Hai region soybean, subgroup III averaged soybean in Huang-Huai-Hai and southern region. Structure genetic structure analysis divided soybean into three different consanguinities. The results of the both analysis showed that there was no obvious regional correlation between the distribution of cultivated soybean.
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