LI Huan-li,MA Yan-bin,LUO Xiao-li,et al.The Effect of Exogenous Silicon on Expression of Stress Related Transcription Factors in Soybean Under Adverse Stress[J].Soybean Science,2020,39(03):352-360.[doi:10.11861/j.issn.1000-9841.2020.03.0352]
外源硅对逆境胁迫下大豆抗逆相关转录因子表达的影响
- Title:
- The Effect of Exogenous Silicon on Expression of Stress Related Transcription Factors in Soybean Under Adverse Stress
- Keywords:
- Soybean; Exogenous silicon; Salt; Drought; NAC transcription factors; WRKY transcription factors
- 文献标志码:
- A
- 摘要:
- 为探究外源硅对逆境胁迫下大豆转录因子的表达模式，分析大豆晋豆37号幼苗在盐、干旱胁迫和外源硅作用下NAC家族GmNAC2、GmNAC3、GmNAC4、GmNAC5以及WRKY家族GmWRKY1、GmWRKY25、GmWRKY38、GmWRKY54转录因子的表达情况。结果表明：在盐、干旱逆境胁迫下，大豆植株生长受抑制，与对照相比，逆境胁迫下的植株鲜重、干重、株高、叶片数、叶片相对含水量均不同程度下降，而加入外源硅后这些指标较单独胁迫明显升高。NAC家族和WRKY家族的8个转录因子在大豆叶、茎和根系均有表达，在盐、干旱胁迫下，GmNAC2、GmNAC3、GmNAC4、GmNAC5以及GmWRKY1、GmWRKY25、GmWRKY38、GmWRKY54的表达量明显低于对照，而在胁迫下加入外源硅后其表达量明显比单独胁迫高，且随逆境胁迫时间延长，转录因子的表达量变化趋势基本一致，不同处理时间单独施加硅处理和对照无显著差异。说明外源硅能够在盐、干旱逆境胁迫下影响大豆相关转录因子的表达。
- Abstract:
- In order to explore the expression analysis of exogenous silicon on soybean transcription factors under adverse stress, this study analyzed the expression of GmNAC2, GmNAC3, GmNAC4, GmNAC5 from NAC transcription factors family and GmWRKY1, GmWRKY25, GmWRKY38, and GmWRKY54 from WRKY family in Jindou 37 soybean seedlings under salt, drought stress and exogenous silicon treatment. The results showed that soybean plant growth was inhibited under salt and drought stress. Compared with the control group, the fresh weight, dry weight, plant height, leaf number and relative water content under stress decreased to different degree, while the addition of exogenous silicon under stress increased significantly compared with the stress alone. The eight transcription factors were all expressed in soybean leaves, stems and roots. Under salt and drought stress, the expression of GmNAC2, GmNAC3, GmNAC4, GmNAC5 and GmWRKY1, GmWRKY25, GmWRKY38, GmWRKY54 was significantly lower than the amount of control, while the expression along with the exogenous silicon under stress was significantly higher than the single stress value, and with prolonged adversity stress, the expression of transcription factor quantity trends were basically identical. There was no significant difference between silicon alone treatment and the control treatment during the different time in the experiment. It is suggested that exogenous silicon can affect the expression of related transcription factors under salt and drought stress.
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