BAO Xuan-ping,WU Yue-ying,LI Min-geng,et al.Genetic Analysis of Soybean Resistance to Thrips flavus[J].Soybean Science,2020,39(02):297-303.[doi:10.11861/j.issn.1000-9841.2020.02.0297]
大豆对黄蓟马抗性遗传分析
- Title:
- Genetic Analysis of Soybean Resistance to Thrips flavus
- Keywords:
- Soybean; Thrips flavus; Insect resistance; Linkage genetic analysis; rtf1
- 文献标志码:
- A
- 摘要:
- 为促进大豆对黄蓟马抗性的研究,同时推动黄蓟马及其它类蓟马的防治工作,本研究利用齐黄34与冀豆17为亲本构建的重组自交系群体,采用黄蓟马自然侵食幼苗的方法对128个株系进行抗虫鉴定,以虫害指数表征各株系苗期抗虫能力,基于各株系基因型进行连锁遗传分析。初步鉴定了3个大豆苗期抗黄蓟马的遗传位点rtf1、rtf2、rtf3,其中,位于大豆11号染色体上的rtf1是主效位点,可解释表型变异的15.8%,且与已报道的大豆抗蚜虫位点qRAP_B1_1位置重合,研究结果能为大豆抗虫机制解析和大豆抗虫品种的选育提供重要参考。
- Abstract:
- In order to promote the study of resistance of soybean to Thrips flavus and promote the control of Thrips flavus and other Thrips, we used the recombinant inbred line population constructed by Qihuang 34 and Jidou 17 as the parents, made the identification of insect-resistance on 128 lines by Thrips flavus invading soybean seedlings naturally. We used the pest index to characterize the insect-resistance of each line, and analysised the linkage genetic based on the genotypes of each line. We initially located 3 soybean Thrips flavus-resistant loci, named rtf1, rtf2 and rtf3, respectively. The locus rtf1 on chromosome 11 is the major one, which explaining 15.8% of the phenotypic variance, and interestingly, it has an overlap region with the soybean aphid-resistant locus qRAP_B1_1. The mapping of soybean Thrips flavus-resistant genetic loci may provide an important reference for the analysis on the mechanism of soybean insect-resistance and the selection and breeding of insect-resistant soybean.
参考文献/References:
[1]高宇, 刘延超, 夏婷婷, 等. 黄蓟马的寄主范围、生物学特性及危害特点综述[J]. 江苏农业科学, 2018, 46(17): 87-90. (Gao Y, Liu Y C, Xia T T, et al. Host range, biological characteristics and hazard characteristics of honeysuckle thrips (Thrips flavus): A review[J]. Jiangsu Agricultural Sciences, 2018, 46(17): 87-90.)[2]戚克耀, 赵宪兴, 王冶. 大豆蓟马的发生危害及防治[J]. 大豆通报, 2002(1): 13. (Qi K Y, Zhao X X, Wang Z. Harm and prevention of soybean trips[J]. Soybean Bulletin, 2002(1): 13.)[3]谭国忠, 李春杰, 许艳丽, 等. 豆黄蓟马的识别与综合防治[J]. 大豆科技, 2008(3): 29-30. (Tan G Z, Li C J, Xu Y L, et al. Identification and integrated managements of Thrips nigropilosus Uzel[J]. Soybean Science & Technology, 2008(3): 29-30.)[4]Singh S J,Krishnareddy M. Thrips flavus Schrank (Thysanoptera: Thripidae), a new insect vector of a tospovirus infecting watermelon in India[J]. Pest Management in Horticultural Ecosystems, 1995, 1(2): 115-118.[5]高宇, 史树森, 崔娟, 等. 三种颜色色板对大豆田蓟马的诱集效果[J]. 中国油料作物学报, 2016, 38(6): 838-842. (Gao Y, Shi S S, Cui J, et al. Trapping effect of three color sticky traps on thrips in soybean fields[J]. Chinese Journal of Oil Crop Sciences, 2016, 38(6): 838-842.)[6]万岩然, 何秉青, 苑广迪, 等. 北京和云南地区西花蓟马对多杀菌素类药剂产生抗药性[J]. 应用昆虫学报, 2016, 53(2): 396-402. (Wan Y R, He B Q, Yuan G D, et al. Development of resistance to spinosyns in populations of western flower thrips in Beijing and Yunnan[J]. Chinese Journal of Applied Entomology, 2016, 53(2): 396-402.)[7]高宇, 刘延超, 史树森, 等. 我国大豆田蓟马研究现状[J]. 作物杂志, 2017(1): 8-13. (Gao Y, Liu Y C, Shi S S, et al. Review on soybean thrips in China[J]. Crops, 2017(1): 8-13.)[8]特木尔布和, 斯琴. 抗蓟马紫花苜蓿形态特征及其抗虫性研究[J]. 内蒙古农业大学学报(自然科学版), 2014(5): 51-58. (Temuerbuhe, Si Q. Morphological characteristics and insect resistance of thrips-resistant alfalfa[J]. Journal of Inner Mongolia Agricultural University (Natural Science Edition), 2014(5): 51-58.)[9]Hill C B, Yan L, Hartman G L. A Single dominant gene for resistance to the soybean aphid in the soybean cultivar dowling[J]. Crop Science, 2006, 46(4): 1601.[10]Kim K S, Hill C B, Hartman G L, et al. Fine mapping of the soybean aphid-resistance gene Rag2 in soybean PI 200538[J]. Theoretical and Applied Genetics, 2010, 121(3): 599-610.[11]Li Y, Hill C B, Carlson S R, et al. Soybean aphid resistance genes in the soybean cultivars Dowling and Jackson map to linkage group M[J]. Molecular Breeding, 2007, 19(1): 25-34.[12]Kim K S,Bellendir S, Hudson K A, et al. Fine mapping the soybean aphid resistance gene Rag1 in soybean[J]. Theoretical & Applied Genetics, 2010, 120(5): 1063-1071.[13]Kang S T,Mian M A R, Hammond R B. Soybean aphid resistance in PI 243540 is controlled by a single dominant gene[J]. Crop Science, 2008, 48(5): 1744-1748.[14]Mian M A R, Kang S T, Beil S E, et al. Genetic linkage mapping of the soybean aphid resistance gene in PI 243540[J]. Theoretical and Applied Genetics, 2008, 117(6):955-962.[15]Zhang G,Gu C, Wang D. A novel locus for soybean aphid resistance[J]. Theoretical & Applied Genetics, 2010, 120(6): 1183-1191.[16]Zhang G,Gu C, Wang D. Molecular mapping of soybean aphid resistance genes in PI 567541B[J]. Theoretical & Applied Genetics, 2009, 118(3): 473-482.[17]Jun T H , Rouf M A , Michel A P . Genetic mapping of three quantitative trait loci for soybean aphid resistance in PI 567324[J]. Heredity, 2013, 111(1): 16-22.[18]Xiao L, Hu Y, Wang B, et al. Genetic mapping of a novel gene for soybean aphid resistance in soybean (Glycine max[L.] Merr.) line P203 from China[J]. Theoretical and Applied Genetics, 2013, 126(9): 2279-2287. [19]黄珊珊. 东北春大豆抗蚜关键酶活性测定、遗传分析及QTL定位[D]. 哈尔滨: 东北农业大学, 2013. (Huang S S. Determination of key enzyme activity, inheritance and QTL analysis of resistance to Soybean Aphid [Aphis glycines Matsumura] in spring soybean of northeast China[D]. Harbin: Northeast Agricultural University, 2013.)[20]徐冉, 王彩洁, 张礼凤, 等. 高产优质多抗广适大豆新品种齐黄34的选育[J]. 山东农业科学, 2013, 45(3): 107-108. (Xu R, Wang C J, Zhang L F, et al. Breeding of high-yield high-quality widespread and multiple disease resistant soybean Qihuang 34[J]. Shandong Agricultural Sciences, 2013, 45(3): 107-108.)[21]赵青松, 闫龙, 刘兵强, 等. 高产广适优质大豆品种冀豆17 [J]. 大豆科学, 2015, 34(4): 736-739,741. (Zhao Q S, Yan L, Liu B Q, et al. Breeding of high-yield widespread and high-quality soybean cultivar Jidou 17[J]. Soybean Science, 2015, 34(4): 736-739,741.)[22]邱丽娟, 常汝镇, 刘章雄, 等. 大豆种质资源描述规范和数据标准[M]. 北京: 中国农业出版社, 2006: 78-79. (Qiu L J, Chang R Z, Liu Z X, et al. Description specification and data standard of soybean germplasm resources[M]. Beijing: Agricultural Press, 2006: 78-79.)[23]郭继红, 于连成, 邵素兰. 黄蓟马在河南周口地区秋田大发生[J]. 植保技术与推广, 1997, 17(5): 40-40. (Guo J H, Yu L C, Shao S L. Thrips flavus erupt in the autumn field in Zhoukou, Henan[J]. Plant Protection Technology and Extension, 1997, 17 (5): 40-40.)[24]赵昶灵, 和钧秋, 武绍波, 等. 危害滇中砀山酥梨花的蓟马种类初报[J]. 落叶果树, 2002, 34(6): 6-7. (Zhao C L, He J Q, Wu S B, et al. Preliminary report on common thrips species harming ‘Dangshansu’ pear flowers [J]. Deciduous Fruits, 2002, 34(6): 6-7.)[25]魏潮生, 梁伟坤. 菜田黄蓟马生物学特性及其防治[J]. 植物保护, 1983(4): 28-28. (Wei C S, Liang W K. Biological characteristics and control of Thrips flavus in vegetable field [J]. Plant Protection, 1983(4): 28-28.)[26]卿贵华. 石榴黄蓟马生物学特性研究[J]. 山地农业生物学报, 2008, 27(5): 402-406. (Qing G H. Biological characteristics of Thrips flavus schrank on Punica Granatum[J]. Journal of Mountain Agriculture and Biology, 2008, 27(5): 402-406.)
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备注/Memo
收稿日期:2019-07-06