[1]吴艳,侯智红,程群,等.大豆GmSPL3基因家族功能初探[J].大豆科学,2019,38(05):694-703.[doi:10.11861/j.issn.1000-9841.2019.05.0694]
　WU Yan,HOU Zhi-hong,CHENG Qun,et al.Preliminary Study on the Function of GmSPL3 Gene Family in Soybean[J].Soybean Science,2019,38(05):694-703.[doi:10.11861/j.issn.1000-9841.2019.05.0694]
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大豆GmSPL3基因家族功能初探

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷 期数: 2019年05期 页码: 694-703 栏目: 出版日期: 2019-09-20
Title:
Preliminary Study on the Function of GmSPL3 Gene Family in Soybean
作者:
吴艳; 侯智红; 程群; 董利东; 芦思佳; 南海洋; 甘卓然; 林永波
（广州大学 生命科学学院，广东 广州 510006）
Author(s):
WU Yan;  HOU Zhi-hong;  CHENG Qun;  DONG Li-dong;  LU Si-jia;  NAN Hai-yang;  GAN Zhuo-ran; LIN Yong-bo
(School of Life Sciences, Guangzhou University, Guangzhou 510006, China)
关键词:
大豆; SPL; 表达谱; 组织特异性表达分析; CRISPR/Cas9; 株高
Keywords:
Soybean;  SPL;  Digital expression profile;  Tissue-specific expression analysis;  CRISPR/Cas9;  Plant height
DOI:
10.11861/j.issn.1000-9841.2019.05.0694
文献标志码:
A
摘要:
为研究大豆中拟南芥(Arabidopsis thaliana)重要开花调控AtSPL3同源基因的作用，本研究利用生物信息学方法分析大豆的RNA-seq基因表达谱数据，对绥农14根 、茎 、子叶、初生叶、三出复叶、花、生长点、荚、胚和下胚轴10个组织进行GmSPL3基因的组织特异性表达分析，并利用CRSPR/Cas9的方法构建大豆GmSPL3基因的四突变体并对比其表型。结果显示：GmSPL3基因在大豆的根、子叶、花和种子中有着明显的表达差异；GmSPL3a在各组织中的表达量都非常低，而GmSPL3（b，c，d）在不同组织中存在明显的组织特异性表达；在GmSPL3基因敲除突变体中研究发现，短日照条件下，spl3abcd突变体表现出叶片变小、节数减少、节间距缩短、株高降低的表型，表明GmSPL3在调控大豆植株形态方面发挥重要功能。
Abstract:
The gene AtSPL3 plays an important role in regulating the flowering process of Arabidopsis thaliana. In this study, we analyzed the gene expression differences in the root, nodule, cotyledon, flower, stem, shoot apex and seed of soybean with digital expression profile, qRT-PCR showed that the expression of GmSPL3a in flower and shoot apex was higher, GmSPL3 (b, c) was highly expressed in leaves, and GmSPL3d was mainly expressed in trifoliolate leaf and shoot apex. It was suggested that the GmSPL3 genes may be involved in the growth and development of soybean. Then, we generated GmSPL3 mutant in Williams 82 (W82) background by gene editing, and investigated the phenotype cause by GmSPL3 loss-of-function. In GmSPL3 knockout mutants, it was found that spl3abcd mutants showed the fewer nodes, shorter internode length and lower plant height under SD, indicating that GmSPL3 played an important role in regulating the plants morphology of soybean.

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备注/Memo

收稿日期：2019-04-10

基金项目：国家自然科学基金（31771815，31701445，31801384）。第一作者简介：吴艳（1993-），女，硕士，主要从事大豆光周期调控开花研究。E-mail：15367613473@163.com。通讯作者：林永波（1966-），男，博士，副教授，硕导，主要从事大豆光周期调控开花研究。E-mail：linyongbo1966@aIiyun.com。

更新日期/Last Update: 2019-09-20