[1]靳路真,王洋,张伟,等.高温胁迫对不同耐性大豆品种生理生化的影响[J].大豆科学,2019,38(01):63-71.[doi:10.11861/j.issn.1000-9841.2019.01.0063]
　JIN Lu-zhen,WANG Yang,ZHANG Wei,et al.Effects of High Temperature Stress on Physiological and Biochemical Traits of Soybeans with Different Heat Tolerance[J].Soybean Science,2019,38(01):63-71.[doi:10.11861/j.issn.1000-9841.2019.01.0063]
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高温胁迫对不同耐性大豆品种生理生化的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷 期数: 2019年01期 页码: 63-71 栏目: 出版日期: 2019-01-20
Title:
Effects of High Temperature Stress on Physiological and Biochemical Traits of Soybeans with Different Heat Tolerance
作者:
靳路真1; 王洋2; 张伟2; 徐晋豫1; 申战士1; 谢甫绨3
(1.延津县农林畜牧局, 河南 新乡 453200； 2.吉林省农业科学院, 吉林 长春 130033； 3.沈阳农业大学 大豆研究所, 辽宁 沈阳 110866）
Author(s):
JIN Lu-zhen1;  WANG Yang2;  ZHANG Wei2;  XU Jin-yu1;  SHEN Zhan-shi1;  XIE Fu-ti3
(1.Yanjin Agriculture, Forestry and Livestock Bureau, Xinxiang 453200, China; 2.Jilin Academy of Agricultural Sciences, Changchun 130033, China; 3.Soybean Research Institute of Shenyang Agricultural University, Shenyang 110866, China)
关键词:
大豆; 高温胁迫; 耐热性; 渗透调节物质; 抗氧化物酶; 叶绿素; 单株粒重
Keywords:
Soybean;  High temperature stress;  Heat tolerance;  Osmotic regulatory substance; Antioxidant enzymes; Chlorophyll; Seed weight per plant
DOI:
10.11861/j.issn.1000-9841.2019.01.0063
文献标志码:
A
摘要:
为了探索高温胁迫对不同耐性大豆品种的影响，在不同生育时期对不同耐热性大豆品种进行高温胁迫处理，研究其对大豆品种生理特性的影响以及各生理指标与单株粒重的相关性。结果表明，高温胁迫下大豆叶片中可溶性糖含量、叶绿素a/b值呈下降趋势，MDA含量和SOD酶活性呈上升趋势。苗期、开花期和鼓粒期40±2℃高温胁迫使长农17可溶性糖含量分别降低43.34%、28.09%和34.25%，使锦州4-1分别降低59.03%、58.66%和45.68%。苗期40±2℃高温胁迫下叶绿素a/b值下降最为明显，锦州4-1叶绿素a/b值下降幅度（40.55%）高于长农17叶绿素a/b值下降幅度（34.40%）。开花期MDA含量变化幅度最大，40±2℃高温胁迫下，锦州4-1的MDA含量增加幅度（135.94%）高于长农17的MDA含量增加幅度（114.86%）。在开花期和鼓粒期高温胁迫下叶片脯氨酸含量呈增加趋势，开花期两品种差异最为明显，40±2℃高温胁迫使长农17脯氨酸含量增加了113.84%，而使锦州4-1脯氨酸含量增加19.61%。在苗期和开花期随胁迫温度的增加ASA含量、POD酶活性呈下降趋势，而在鼓粒期高温胁迫下则呈增加趋势。单株粒重与大豆叶片可溶性糖含量和叶绿素a/b值呈极显著正相关，与MDA含量呈显著负相关。综上可得，高温胁迫下耐热品种长农17的可溶性糖含量、叶绿素a/b值及SOD酶活性均高于热敏感品种锦州4-1，MDA含量低于热敏感品种锦州4-1。35±2℃、40±2℃高温胁迫下，耐热性品种长农17的脯氨酸含量、ASA含量、SOD以及POD酶活性水平高于热敏感品种锦州4-1，耐热品种长农17在高温胁迫下仍能保持较高的单株粒重，且品种间差异显著。
Abstract:
In order to explore the effects of high temperature stress on different heat-resistant soybean, in this study, different heat-resistant soybean varieties were treated under high temperature stress at different growth stages, and the physiological and biochemical traits of soybean varieties and the correlation between physiological characters and seed weight per plant were studied. The results showed that soluble sugar content, Chl a/b value decreased, MDA content and SOD activity increased with the increase of stress temperature at different growth stages. Under the treatment of 40±2℃ in seedling stage, flowering stage and seed-filling stage, the content of soluble sugar of Changnong 17 decreased by 43.34%, 28.09% and 34.25% respectively, and the content of soluble sugar of of Jinzhou 4-1 decreased by 59.03%, 58.66% and 45.68% respectively. The Chl a/b value under the treatment of 40±2℃ at seeding stage decreased most obviously. The decrease of Chl a/b value of Jinzhou 4-1 (40.55%) exceeded the decrease of Chl a/b value of Changnong 17 (34.40%). The increase of MDA content under the treatment of 40±2℃ at the flowering stage was the highest. The increase of MDA content of Jinzhou 4-1 (135.94%) exceeded the increase of MDA content of Changnong 17 (114.86%). The content of proline in leaves increased with the increase of stress temperature at flowering stage and seed-filling stage, and the most obvious difference between the two varieties under the treatment of 40±2℃was that the proline content increased by 113.84% for Changnong 17 and by 19.61% for Jinzhou 4-1. The activity of POD and ASA content decreased with the increase of stress temperature at seedling stage and flowering stage, and increased at seed filling stage. Grain weight per plant was extremely a significant positive correlation with soluble sugar content and Chl a/b value, and significant negative correlation with MDA content. In conclusion, the soluble sugar content, Chl a/b value and SOD activity of heat-resistant variety Changnong 17 were all higher than those of heat-sensitive variety Jinzhou 4-1, and the MDA content of heat-resistant variety Changnong 17 was lower than that of heat-sensitive variety Jinzhou 4-1. Proline content, ASA content, SOD activity and POD activity of Changnong 17 were still higher than those of Jinzhou 4-1 under the treatment of 35±2℃, 40±2℃. Seed weight per plant of heat-resistant variety Changnong 17 still kept a higher level, and there was a significant difference between the varieties.

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备注/Memo

收稿日期：2018-09-07

基金项目：国家重点研发计划（2017YFD0101306-04）;中央引导地方项目（2018416023）。
第一作者简介：靳路真(1990-), 女, 硕士, 助理农艺师, 主要从事大豆栽培和示范推广研究。E-mail:909581309@qq.com。
通讯作者：谢甫绨(1968-),男,教授,博导,主要从事大豆育种与栽培生理研究。 E-mail:snsoybean@sohu.com。

更新日期/Last Update: 2019-01-22