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[1]于奇,曹亮,金喜军,等.低温胁迫下褪黑素对大豆种子萌发的影响[J].大豆科学,2019,38(01):56-62.[doi:10.11861/j.issn.1000-9841.2019.01.0056]
　YU Qi,CAO Liang,JIN Xi-jun,et al.Effects of Melatonin on Seed Germination of Soybean under Low temperature Stress[J].Soybean Science,2019,38(01):56-62.[doi:10.11861/j.issn.1000-9841.2019.01.0056]

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低温胁迫下褪黑素对大豆种子萌发的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷期数: 2019年01期页码: 56-62 栏目: 出版日期: 2019-01-20

Title:: Effects of Melatonin on Seed Germination of Soybean under Low temperature Stress

作者:: 于奇; 曹亮; 金喜军; 邹京南; 王孟雪; 张明聪; 任春元; 张玉先; (黑龙江八一农垦大学农学院，黑龙江大庆 163319)

Author(s):: YU Qi; CAO Liang; JIN Xi-jun; ZOU Jing-nan; WANG Meng-xue; ZHANG Ming-cong; REN Chun-yuan; ZHANG Yu-xian; (Agronomy College of Heilongjiang Bayi Agricultural University, Daqing 163319, China)

关键词:: 低温; 大豆; 褪黑素; 浸种; 萌发

Keywords:: Low temperature; Soybean; Melatonin; Soaking seeds; Germination

DOI:: 10.11861/j.issn.1000-9841.2019.01.0056

文献标志码:: A

摘要:: 为研究低温胁迫下褪黑素对大豆萌发的影响，促进褪黑素在实际生产中的应用，以绥农26为供试品种，大豆种子分别在常温(25℃)下萌发24 h(T₂₄)和48 h(T₄₈)后转移至4℃低温胁迫处理24 h，而后转移至常温继续发芽至7 d结束，研究不同浓度褪黑素浸种处理（0，50，100，200，400 μmol·L^-1）对大豆种子萌发的影响。结果表明：与未用褪黑素浸种相比，200 μmol·L^-1褪黑素浸种处理可显著提高T₂₄条件下第3天和第4天大豆发芽率，提高幅度分别为42%和29%，而在T₄₈条件下则表现为100 μmol·L^-1处理显著提高了第4 天和第5天大豆发芽率，提高幅度分别为20%和39%；100 μmol·L-1处理可显著提高T₂₄和T₄₈条件下发芽指数和芽鲜重，提高幅度分别为24.6%和29.6%，24.7%和37%；200和400 μmol·L^-1处理可显著提高T₂₄和T₄₈条件下大豆芽SOD活性，提高幅度分别为281%和304%，100 μmol·L^-1处理可显著提高T₂₄和T₄₈条件下CAT、POD、APX活性、脯氨酸和可溶性蛋白含量，提高幅度分别为86%和700%，138%和96%，500%和200%，184%和230%，70%和32%；可溶性糖含量在T₂₄和T₄₈条件下表现为200和400 μmol·L^-1处理最高，而MDA含量则表现为100 μmol·L^-1降低幅度最大。综合发芽率、芽形态和生理相关指标分析认为，褪黑素浓度100～200 μmol·L^-1效果最显著。

Abstract:: To study the effect of melatonin on soybean germination under low temperature stress, and promoted the application of melatonin in actual production, in this study, Suinong 26 was used as the test variety. Soybean seeds were germinated at room temperature (25°C) for 24 h (T₂₄) and 48 h (T₄₈), then transferred to 4°C for 24 h, then transferred to room temperature to continued germination. At the end of 7 d, the effects of different concentrations of melatonin soaked treatment (0, 50, 100, 200, 400 μmol·L^-1) on the germination of soybean seeds were studied. The results showed that 200 μmol·L^-1 melatonin soaked treatment significantly increased soybean germination rate compared with melatonin soaking on the third and fourth days under T₂₄conditions, with an increase of 42% and 29%, respectively. While under the condition of T₄₈, the performance of 100 μmol·L^-1 significantly increased the germination rate of soybeans on the 4th and 5th day, respectively, with an increase of 20% and 39%.100 μmol·L-1 treatment significantly increased the germination index and bud fresh weight under the conditions of T₂₄ and T₄₈, the increase rate was 24.6% and 29.6%, 24.7% and 37% respectively. The treatment of 200 and 400 μmol·L^-1 could significantly improve soybean bud SOD activity under T₂₄ and T₄₈ conditions increased by 281% and 304%, respectively. Treatment with 100 μmol·L-1 significantly increased CAT, POD, APX activity, proline and soluble protein content under T₂₄and T₄₈ conditions, increased by 86% and 700%, 138% and 96%, 500% and 200%, 184% and 230%, 70% and 32%, respectively. Soluble sugars content showed the highest treatment at 200 and 400 μmol·L^-1under T₂₄ and T₄₈ conditions, while the MDA content showed the largest decrease at 100 μmol·L^-1. According to the analysis of comprehensive germination rate, bud morphology and physiological correlation index, the melatonin concentration of 100~200 μmol·L^-1 was the most significant.

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备注/Memo

收稿日期：2018-09-20

基金项目：国家重点研发计划（2018YFD0201000）；国家现代农业产业技术体系(CARS-04-01A)；黑龙江省自然科学基金（C2017049）；黑龙江省农垦总局重点科研计划（HNK135-02-06）。

第一作者简介：于奇（1994-），女，硕士，主要从事大豆逆境栽培生理研究。E-mail：1107139112@qq.com。

通讯作者：张玉先（1968-），男，教授，博导，主要从事大豆逆境栽培生理和轮作体系研究。E-mail：zyx_lxy@126.com。

更新日期/Last Update: 2019-01-22