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[1]李剑桥,张逢凯,邢光南,等.不同生育阶段剪叶量对大豆品种南农99-6农艺和品质性状的影响[J].大豆科学,2018,37(05):715-722.[doi:10.11861/j.issn.1000-9841.2018.05.0715]
　LI Jian-qiao,ZHANG Feng-kai,XING Guang-nan,et al.Influence of Different Defoliation Rates at Different Growth Stages to Agronomic and Quality Traits of Soybean Cultivar NN99-6[J].Soybean Science,2018,37(05):715-722.[doi:10.11861/j.issn.1000-9841.2018.05.0715]

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不同生育阶段剪叶量对大豆品种南农99-6农艺和品质性状的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷期数: 2018年05期页码: 715-722 栏目: 出版日期: 2018-09-20

Title:: Influence of Different Defoliation Rates at Different Growth Stages to Agronomic and Quality Traits of Soybean Cultivar NN99-6

作者:: 李剑桥; 张逢凯; 邢光南; 盖钧镒; （南京农业大学大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室(综合)/作物遗传与种质创新国家重点实验室/江苏省现代作物生产协同创新中心，江苏南京 210095)

Author(s):: LI Jian-qiao; ZHANG Feng-kai; XING Guang-nan; GAI Jun-yi; (Soybean Research Institute of Nanjing Agricultural University/ National Center for Soybean Improvement/ Key Laboratory for Biology and Genetic Improvement of Soybean (General), Ministry of Agriculture/ National Key Laboratory for Crop Genetic and Germplasm Enhancement/ Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210095, China)

关键词:: 大豆; 食叶性害虫; 剪叶量; 生育阶段; 农艺性状; 品质性状

Keywords:: Soybean; Leaf feeding insect; Defoliation rate; Growth stage; Agronomic traits; Seed quality traits

DOI:: 10.11861/j.issn.1000-9841.2018.05.0715

文献标志码:: A

摘要:: 筛选大豆对叶片机械损失敏感的指标和时期，为评价大豆对食叶性害虫的耐虫性及制定虫害防治指标等提供参考。在大豆品种南农99-6不同生育阶段（V5、R1、R3、R5）进行不同程度（0%、16.7%、33.3%、50.0%、66.7%、83.3%和100%）的剪叶处理，测定农艺和品质性状，以探究不同生育阶段不同叶片损失程度对农艺和品质性状的影响。结果表明：（1）不同剪叶量处理间，单株产量、单株荚数、四粒荚数、三粒荚数、二粒荚数和株高差异达极显著水平，主茎节数、一粒荚数和百粒重差异达显著水平，有效分枝数、每荚粒数及蛋白质含量和油脂含量没有显著差异；一粒荚、二粒荚、三粒荚和四粒荚的数量都有随剪叶量增加逐步减少的趋势。（2）平均单次33.3%及以上的叶片损失可导致单株荚数显著减少；50%及以上的叶片损失可导致单株产量显著降低；而100%叶片损失可导致百粒重显著降低。（3）不同生育期剪叶处理间百粒重、三粒荚数、单株荚数和单株产量差异达到或接近显著水平，而其它性状无显著差异。始荚期的叶片损失对单株荚数影响最大，而鼓粒始期的叶片损失对百粒重影响最大且同时影响单株荚数，因而R3和R5是叶片损失的敏感时期。（4）不同生育阶段与不同剪叶量间的互作对大豆农艺和品质性状的影响较小，都未达到显著水平。（5）该试验结果模拟了大豆受咀嚼式食叶性害虫危害的机械损伤影响，但不涉及取食时分泌唾液毒害和刺吸式口器害虫吸食的影响。（6）建议用虫害关键时期（R3）33.3%的剪叶量模拟咀嚼式食叶性害虫危害，以单株荚数损失率、百粒重损失率及单株产量损失率作为评价指标鉴定大豆的耐虫性。

Abstract:: To provide a reference for evaluating soybean tolerance against leaf-feeding insects and formulating pest control indicators, indicators and periods sensitive to mechanical loss of the blade are screened. The effects of different defoliation rates (0%, 16.7%, 33.3%, 50.0%, 66.7%, 83.3% and 100%) at different growth stages (V5, R1, R3, R5) on soybean cultivar NN99-6 were evaluated to explore their influences to agronomic and quality traits. (i) Highly significant differences were found among different defoliation rates for pod number per plant, seed yield per plant, plant height and for number of two-, three- and four-seed pods, significant differences were found for number of nodes on main stem, number of one-seed pods and 100-seed weight, while there were no significant differences among effective branch number, seeds number per pod, protein and oil content. The number of one-, two-, three- and four-seed pods decreased gradually along with the increase of defoliation rates. (ii) On average, a 33.3% and above defoliation could lead to a significant reduction in pod number per plant, a 50% and above defoliation could lead to a significant decrease in seed yield per plant, while a 100% defoliation could result in a significant decrease in 100-seed weight. (iii) Among different defoliation stages, there showed significant or approximately significant differences for 100-seed weight, three-seed pod number, pod number per plant and seed yield per plant, while no significant difference was found for other traits. Defoliation at beginning podding stage (R3) caused greatest influences on pod number per plant. While defoliation at the beginning of seed development stage (R5) caused the greatest influence on 100-seed weight and significant influenced on pod number per plant. Therefore, R3 and R5 were critical periods of leaf loss. (iv) The interactions between defoliation stages and defoliation rates showed a little influence on agronomic and quality traits, and did not reach a significant level. (v) This experiment simulated the mechanical damage of soybeans caused by leaf-chewing insects, but did not involve the effects due to saliva poisoning through feeding and sucking by phloem-sucking insects. (vi) It is suggested that 33.3% defoliation rate can be used to simulate damage of leaf-chewing insects at R3 stage which is a critical period for leaf-feeder damages, and the loss rate of pod number per plant, 100-seed weight and seed yield per plant can be used as indicators of tolerance to leaf-feeding insects.

参考文献/References:

［1］Srinivasan V, Kumar P, Long S P. Decreasing, not increasing, leaf area will raise crop yields under global atmospheric change ［J］. Global Change Biology, 2016, 23(4): 1626-1635.

［2］齐灵子. 大豆食叶害虫危害损失预测模型的研究［D］. 长春: 吉林农业大学, 2013. (Qi L Z. Soybean defoliator hazard loss forecast model research ［D］.Changchun: Jilin Agricultural University, 2013.)

［3］王永锋, 郝聪慧, 马赛飞, 等. 大豆不同生育期去叶对其生长发育及产量的影响［J］. 安徽农业科学, 2003, 31(3): 440-442. (Wang Y F, Hao C H, Ma S F, et al. Effects of leaf removal on growth and yield of soybean at different growth stages ［J］. Journal of Anhui Agricultural Sciences, 2003, 31(3): 440-442.)

［4］许志春, 李凯, 李镇宇, 等. 油松对松毛虫危害的补偿机制研究［J］. 北京林业大学学报, 1996, 18(1): 61-65. (Xu Z C, Li K, Li Z Y, et al. Compensative mechanism of Chinese pine damaged by pine caterpillars ［J］. Journal of Beijing Forestry University, 1996, 18(1): 61-65.)

［5］王志明, 刘国荣. 长白落叶松对叶部病虫危害的补偿与超补偿效应的研究［J］. 林业科学研究, 2006, 19(5): 625-628. (Wang Z M, Liu G R. The effects of compensation and extra compensation of Larix olgensis for needle pests injury ［J］. Forest Research, 2006, 19(5): 625-628.)

［6］程忠方, 沈卫新, 朱明泉, 等. 水稻对二化螟害补偿作用的研究［J］. 浙江农业科学, 1999(2): 90-92. (Cheng Z F, Shen W X, Zhu M Q, et al. Study on compensation of rice varieties to striped stem borer ［J］. Zhejiang Agricultural Sciences, 1999(2): 90-92.)

［7］崔章林, 盖钧镒, 吉东风, 等. 南京地区大豆食叶性害虫种类调查与分析［J］. 大豆科学, 1997, 16(1): 13-21. (Cui Z L, Gai J Y, Ji D F, et al. A study on leaf-feeding insect species on soybeans in Nanjing area［J］. Soybean Science, 1997, 16(1): 13-21.)

［8］管致和. 农业昆虫学第三章害虫怎样加害农作物［J］. 植物保护, 1982(3):42-43. (Guan Z H. Agricultural entomology chapter III how pests harm crops ［J］. Plant Protection, 1982,8(3):42-43.)

［9］Flore J A, Irwin C. The influence of defoliation and leaf injury on leaf photosynthetic rate, diffusive resistance, and whole tree dry matter accumulation in apple［J］. HortScience, 1983, 18(1): 72.

［10］Boucher T J, Pfeiffer D G, Barden J A, et al. Effects of simulated insect injury on net photosynthesis of potted grapevines ［J］. Hort-Science, 1987, 22(1): 927-928.

［11］Layne D R, Flore J A. Photosynthetic compensation to partial leaf area reduction in sour cherry ［J］. Journal of the American Society for Horticultural Science, 1992, 117(2): 279-286.

［12］何学友, 蔡守平, 谢一青，等. 不同叶面积损失对油茶产量及品质的影响［J］. 林业科学, 2013, 49(5): 85-91. (He X Y, Cai S P, Xie Y Q, et al. Effects of partial leaf area reduction on yield and quality of Camellia oleifera［J］. Scientia Silvae Sinicae, 2013, 49(5): 85-91.)

［13］Macedo T B, Peterson R K D, Dausz C L, et al. Photosynthetic responses of wheat,Triticum aestivum L., to defoliation patterns on individual leaves ［J］. Environmental Entomology, 2007, 36(3): 602-608.

［14］Stacey D L. The effect of artificial defoliation on the yield of tomato plants and its relevance to pest damage ［J］. Journal of Horticultural Science, 1983, 58(1): 117-120.

［15］Yuan R, Alferez F, Kostenyuk I, et al. Partial defoliation can decrease average leaf size but has little effect on orange tree growth, fruit yield and juice quality ［J］. HortScience, 2005, 40(7): 2011-2015.

［16］邱丽娟, 常汝镇, 刘章雄, 等. 大豆种质资源描述规范和数据标准［M］. 北京: 中国农业出版社, 2006. (Qiu L Q, Chang N Z, Liu Z X, et al. Descriptors and data standard for soybean ［M］. Beijing: China Agriculture Press, 2006.)

［17］李媛媛, 张凯, 李霜雯, 等. 失叶率对小黑杨和兴安剪叶松幼苗生物量和叶绿素含量的影响［J］. 林业科学, 2015, 51(3): 93-101. (Li Y Y, Zhang K, Li S W, et al. Effects of defoliations on the chlorophyll contents and biomass of the poplar (Populus simonii×P．nigra) and Larix gmelinii seedlings［J］. Scientia Silvae Sinicae, 2015, 51(3): 93-101.)

［18］胡展育. 植物受虫害后的补偿作用［J］. 文山学院学报, 2007, 20(4): 106-109. (Hu Z Y. Compensatory effect of plant after pest damage ［J］. Journal of Wenshan Teachers College, 2007, 20(4): 106-109.)

［19］Goto H, Shimada H, Horak M J, et al.Characterization of natural and simulated herbivory on wild soybean (Glycine soja Seib. et Zucc.) for use in ecological risk assessment of insect protected soybean［J］. Plos One, 2016, 11(3): e0151237.

［20］Johnson A W. Variations in flue-cured tobacco losses from different tobacco budworm infestation levels［J］. Journal of Economic Entomology, 1975, 68(3): 418-420.

［21］陈永年, 马骏, 袁哲明, 等. 春甘蓝外叶和球叶损失对产量的影响及主要食叶害虫防治行动阈值［J］. 湖南农业大学学报(自科版), 2002, 28(4): 308-313. (Chen Y N, Ma J, Yuan Z M, et al. Study on the influence of artificial defoliation on the yield of common cabbage and the action thresholds for main leaf-mass consuming insect ［J］. Journal of Hunan Agricultural University(Natural Sciences), 2002, 28(4): 308-313.)

［22］王茜, 刘荣堂, 胡桂馨, 等. 牛角花齿蓟马为害对苜蓿株高和分枝的影响［J］. 草原与草坪, 2008(4): 39-41. (Wang Q, Liu R T, Hu G X, et al. Effects of Odontothrips loti damages on alfalfa height and branch［J］. Grassland and Turf, 2008(4): 39-41.)

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备注/Memo

收稿日期：2018-06-21

基金项目：国家重点研发计划项目子课题（2016YFD0100201-22）；国家自然科学基金(31571694)；中央高校基本科研业务费专项（KYT201801）；长江学者和创新团队发展计划（PCSIRT_17R55）；农业部公益性行业专项(201203026-4)；教育部111项目(B08025)；国家现代农业产业技术体系（CARS-04）；江苏省优势学科建设工程专项；江苏省JCIC-MCP项目。

第一作者简介：李剑桥（1993-），男，硕士，主要从事大豆抗虫育种研究。E-mail：2015101136@njau.edu.cn。

通讯作者：邢光南（1980-），男，副教授，主要从事大豆抗虫育种研究。E-mail：xinggn@njau.edu.cn。

盖钧镒（1936-），男，教授，主要从事大豆种质资源与遗传育种研究。E-mail: sri@njau.edu.cn。

更新日期/Last Update: 2018-10-08