[1]张恩柱,赵伟,张淋淋,等.大豆花叶病毒侵染性克隆研究进展[J].大豆科学,2017,36(05):808-812.[doi:10.11861/j.issn.1000-9841.2017.05.0808]
　ZHANG En-zhu,ZHAO Wei,ZHANG Lin-lin,et al.Advances in Infectious Cloning of Soybean Mosaic Virus[J].Soybean Science,2017,36(05):808-812.[doi:10.11861/j.issn.1000-9841.2017.05.0808]
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大豆花叶病毒侵染性克隆研究进展

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年05期 页码: 808-812 栏目: 出版日期: 2017-09-20
Title:
Advances in Infectious Cloning of Soybean Mosaic Virus
作者:
张恩柱1; 赵伟2; 张淋淋1; 韩庆玥1; 张俊华1
(1.东北农业大学 农学院，黑龙江 哈尔滨 150030； 2.黑龙江出入境检验检疫局，黑龙江 哈尔滨 150001）
Author(s):
ZHANG En-zhu1;  ZHAO Wei2;  ZHANG Lin-lin1;  HAN Qing-yue1;  ZHANG Jun-hua1
(1.Agronomy College, Northeast Agricultural University, Harbin 150030, China; 2.Heilongjiang Entry-exit Inspection and Quarantine Bureau, Harbin 150001, China)
关键词:
大豆; 大豆花叶病毒; 侵染性克隆
Keywords:
Soybean;  Soybean mosaic virus;  Infectious clones
DOI:
10.11861/j.issn.1000-9841.2017.05.0808
文献标志码:
A
摘要:
大豆花叶病毒(soybean mosaic virus，SMV)是一种株系复杂且不断变化、并对大豆的产量和质量造成恶劣影响的病毒，其严重的致病性受到学者的普遍关注。大豆花叶病毒侵染性克隆技术是研究其致病机理的行之有效的手段。该技术通过病毒侵染获得侵染性cDNA克隆，进一步分析引起症状的相应基因在植株生长过程中的作用机制，由此为SMV相关领域的研究奠定理论基础。本文就大豆花叶病毒侵染性克隆的构建策略和影响因素，以及其应用状况作一综述，以期为大豆花叶病毒的防治提供新的借鉴和思路。
Abstract:
Soybean mosaic virus (SMV) is a complex and ever-changing virus that has a negative effect on the yield and quality of soybeans. Its serious pathogenicity is generally concerned by scholars associated with the field of soybean research. The SMV is mainly infected with soybean aphids. The research technique of soybean mosaic virus infection cloning was effective and widely adopted to study its pathogenic mechanism. This technique can further analyze the mechanism of the genes that cause the corresponding symptoms in the process of plant growth through the obtained infectious cDNA clones. At the same time, prepared monoclonal antibody can be used as the development of soybean mosaic virus detection kit probe material.As a basis for the study of SMV related fields, based on the relationship between soybean mosaic virus disease and SMV infection cloning technique.This paper briefly summarizes the construction strategy and influencing factors of SMV infectious clone, and summarizes the application of this technology in recent years, to provide new reference and thought for the control of soybean mosaic virus.

参考文献/References:

［1］Gardner M W, Kendrick J B. Soybean mosaic virus［J］. Journal of Agricultural Research, 1921, 2(10): 111-114.

［2］杨永庆. 大豆花叶病毒重组型分离物的鉴定及抗大豆花叶病毒基因的定位及功能分析［D］.南京:南京农业大学, 2012.(Yang Y Q. Identify soybean mosaic virus-recombination isolate and mapping and functional analysis of SMV resistance gene［D］. Nanjing: Nanjing Agricultural College, 2012.)
［3］吴海祥, 张楚瑜. 正链RNA病毒基因组感染性克隆研究进展［J］. 武汉大学学报(理学版), 2002, 48(4): 493-499. (Wu H X, Zhang C Y. Research advancements of genomic infectious cDNA clone of positive-strand RNA virus［J］. Journal of Wuhan University(Natural Science Edition), 2002, 48(4): 493-499.)
［4］智海剑, 盖钧镒. 大豆花叶病毒及抗性遗传的研究进展［J］. 大豆科学, 2006, 25(2): 174-180. (Zhi H J, Gai J Y. Advances in the studies on soybean mosaic virus［J］. Soybean Science, 2006, 25(2): 174-180.)
［5］张锴, 王宇, 王涛, 等. 大豆花叶病毒引发大豆不同症状的生理特性比较［J］. 植物病理学报, 2016, 46(4): 1-8. (Zhang K, Wang Y, Wang T, et al.Comparison of physiological property on soybean leaves showing different symptoms induced by soybean mosaic virus［J］. Acta Phytopathologica Sinica, 2016, 46(1): 1-8.)
［6］李凯, 夏迎春, 王大刚, 等. 黑龙江省大豆花叶病毒(SMV)株系的动态变化分析［J］. 大豆科学, 2014, 33(6): 880-884. (Li K, Xia Y C, Wang D G, et al. Analysis of dynamic change of soybean mosaic virus strains in Heilongjiang province of China［J］. Soybean Science, 2014, 33(6):880-884.)
［7］郑翠明. 大豆对SMV3号株系的抗性遗传及分子标记研究［D］. 北京: 中国农业科学院, 2000.(Zheng C M. Inheritance of resistance to SMV3 and identification of molecular markers linked to resistance gene in soybean［D］. Beijing: Chinese Academy of Agricultural Sciences, 2000.)
［8］王大刚, 张磊, 智海剑. 大豆花叶病毒株系鉴定与分子生物学研究进展［J］. 大豆科学, 2012, 31(4): 668-674. (Wang D G, Zhang L, Zhi H J. Advances in identification of strains and molecular biology of soybean mosaic virus［J］. Soybean Science, 2012, 31(4): 668-674.)
［9］李凯. 中国南方大豆花叶病毒株系的鉴定、抗性遗传和抗性基因的定位［D］. 南京: 南京农业大学, 2009. (Li K. Strain identification of soybean mosaic virus and inheritance and gene mapping of its resistance in soybeans in Southern China［D］. Nanjing: Nanjing Agricultural University, 2009.)
［10］Li K, Yang Q H, Zhi H J, et al. Identification and distribution of soybean mosaic virus strains in Southern China［J］. Plant Disease, 2010, 94(3): 351-357.
［11］王大刚, 田震, 李凯, 等. 鲁豫皖大豆产区大豆花叶病毒株系的鉴定及动态变化分析［J］. 大豆科学, 2013, 32 (6): 806-809．(Wang D G, Tian Z, Li K, et al. Identification and variation analysis of soybean mosaic virus strains in Shandong, Henan and Anhui provinces of China［J］. Soybean Science, 2013, 32(6): 806-809.
［12］杨永庆, 侯文焕, 边全楽, 等. 河北地区大豆花叶病毒株系的组成与分布［J］. 大豆科学, 2014, 33(1): 87-90. (Yang Y Q, Hou W H, Bian Q L, et al. Composition and distribution of SMV strainsin Hebei［J］. Soybean Science, 2014, 33(1): 87-90.)
［13］李凯, 夏迎春, 王大刚, 等. 黑龙江省大豆花叶病毒(SMV)株系的动态变化分析［J］. 大豆科学, 2014, 33(6): 880-884. (Li K, Xia Y C, Wang D G, et al. Analysis of dynamic change of soybean mosaic virus strains in Heilongjiang province of China［J］. Soybean Science, 2014, 33(6): 880-884.)
［14］Boyer J C, Haenni A L. Infectious transcripts and cDNA clones of RNA viruses［J］. Virology, 1994, 198(2): 415-426.
［15］Ahlquist P, French R, Janda M, et al. Multicomponent RNA plant virus infection derived from cloned viral cDNA［J］. Proceedings of the National Academy of Sciences, 1984, 81(22): 7066-7070.
［16］Nagyová A, Subr Z. Infectious full-length clones of plant viruses and their use for construction of viral vectors［J］. Acta Virologica, 2007, 51: 223-237.
［17］Orílio A F, Fortes I M, Navas-Castillo J. Infectious cDNA clones of the crinivirus tomato chlorosis virus are competent for systemic plant infection and white fly-transmission［J］. Virology, 2014, 464: 365-374.
［18］Wang X, Eggenberger A L, Nutter F W, et al. Pathogen-derived transgenic resistance to soybean mosaic virus in soybean［J］. Molecular Breeding, 2001, 8(2): 119-127.
［19］Furutani N, Hidaka S, Kosaka Y, et al. Coat protein genemediated resistance to soybean mosaic virus in transgenic soybean［J］. Breeding Science, 2006, 56(2):119-124.
［20］Fire A, Xu S, Montgomery M K, et a1. Potent and specific genetic interference by doublestranded RNA in Caenorhabditis elegans［J］. Nature, 1998, 391:806-811.
［21］徐俊, 毛颖, 苗荻, 等. RNA干扰技术的研究进展［J］.生物技术世界, 2012(3): 15-17. (Xu J, Mao Y, Miao D, et al. Advances in RNA interference technology［J］. Biotech World, 2012(3): 15-17.
［22］Whitham S A, Lincoln L M, ChowdaReddy R V, et al. Virus-induced gene silencing and transient gene expression in soybean (Glycine max) using bean pod mottle virus infectious clones［J］. Current Protocols in Plant Biology, 2016(1): 263-283.
［23］高乐, 翟锐, 丁雪妮, 等. 大豆花叶病毒CP基因RNAi载体的构建及大豆遗传转化［J］. 植物保护学报, 2014, 41(4): 453-460. (Gao L, Zhai R, Ding X N, et al. RNAi vector construction of soybean mosaic virus CP gene and soybean transformation［J］. Acta Phytophylacica Sinica, 2014, 41(4): 453-460.)
［24］郗冀.新型水貂阿留申病毒感染性克隆的构建及其生物学特性鉴定［D］. 北京: 中国农业大学, 2016. (Xi J.Construction of neotype aleutian mink disease virus infectious clone and identification of its biological characteristics［D］.Beijing: China Agricultural University, 2016.)
［25］Gritsun T S, Could E A. Development and analysis of a tick borne encephalitis infectious clone using a novel and rapid strategy［J］. Journal of Virological Methods, 1998, 76: 109-120.
［26］黄耀伟, 李龙, 于涟. 人类及动物RNA病毒的反向遗传系统［J］. 生物工程学报, 2004, 20(3): 311-318. (Huang Y W, Li L, Yu L. The reverse genetics systems for human and animal RNA viruses［J］. Chinese Journal of Biotechnology, 2004, 20(3): 311-318.)
［27］申识川, 冯力, 时洪艳, 等. 正链RNA病毒感染性克隆的构建策略及影响因素［J］.东北农业大学学报, 2009, 39(8): 134-139. (Shen S C, Feng L, Shi H Y, et al. Construction strategy and influence factors of positive-strand RNA virus infectious clone［J］. Journal of Northeast Agricultural University, 2009, 39(8): 134-139.)
［28］Melton D A, Krieg P A, Rebagliati M R, et al. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter［J］. Nucleic Acids Research, 1984, 12(18): 7035-7056.
［29］Dunn J J, Studier F W, Gottesman M. Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements［J］. Journal of Molecular Biology, 1983, 166(4): 477-535.
［30］Carvalho S L, Nagata T, Junqueira B R, et al. Construction of a full-length infectious cDNA clone of Cowpea mild mottle virus［J］. Virus Genes, 2017, 53(1): 137-140.
［31］Weiland J J, Dreher T W. Infectious TYMV RNA from cloned cDNA: Effects in vitro and in vivo of point substitutions in the initiation codons of two extensively overlapping ORFs［J］. Nucleic acids research, 1989, 17(12): 4675-4687.
［32］司伏生. 猪戊型肝炎病毒分子流行病学分析及基因3型猪戊型肝炎病毒感染性克隆的构建［D］. 南京: 南京农业大学, 2011. (Si F S. Analysis of molecular epidemiology of swine hepatitis e virus (HEV) in Shanghai and construction of infectious cDNA clone of swine genotype 3HEV［D］. Nanjing: Nanjing Agricultural University, 2011.
［33］高瑞.烟草脉带花叶病毒侵染性克隆的构建及其在交叉保护、外源蛋白表达及VIGS中的应用［D］. 泰安: 山东农业大学, 2012. (Gao R. Construction of an infectious cDNA clone of Tobacco vein banding mosaic virus and its use for cross-protection, protein over-expression and VIGS［D］. Taian: Shangdong Agricultural University, 2012.)
［34］谈珺. 黄瓜花叶病毒M株系侵染性cDNA克隆的构建及3a基因的原核表达［D］. 长沙: 湖南农业大学, 2007. (Tan J.Construction of infectious cDNA clone of cucumber mosaic virus strain M and 3a gene expressed in escherichia coli［D］. Changsha: Hunan Agricultural University, 2007.)
［35］Hamilton W D O, Baulcombe D C. Infectious RNA produced by in vitro transcription of a full-length tobacco rattle virus RNA1 cDNA［J］ Journal of General Virology, 1989, 70(4): 963-968.
［36］Kuhn R J, Hong Z, Strauss J H. Mutagenesis of the 3′nontranslated region of Sindbis virus RNA［J］. Journal of Virology, 1990, 64(4): 1465-1476.
［37］Domingo E. Mutation rates and rapid evolution of RNA viruses［J］. The Evolutionary Biology of Viruses, 1994: 161-184.
［38］Ramirez B C, Garcin D, Calvert L A, et al. Capped nonviral sequences at the 5′end of the mRNAs of rice hoja blanca virus RNA4［J］. Journal of Virology, 1995, 69(3): 1951-1954.
［39］Chiba M, Reed J C, Prokhnevsky A I, et al.Diverse suppressors of RNA silencing enhance agroinfection by a viral replicon［J］. Virology, 2006, 74(1): 7-14.
［40］Liu Z, Kearney C M. A tobamovirus expression vector for agroinfection of legumes and Nicotiana［J］. Journal of Biotechnology, 2010, 147(3): 151-159.
［41］Yoon J Y, Choi S K, Palukaitis P, et al.Agrobacterium-mediated infection of whole plants by yellow dwarf viruses［J］. Virus Research, 2011, 160(1): 428-434.
［42］孙浩华, 薛峰, 陈集双. 大豆花叶病毒研究进展［J］. 生命科学, 2007, 18(3): 338-345. (Sun H H, Xue F, Chen J S. Advances in study on soybean mosaic virus (SMV)［J］. Chinese Bulletin of Life Sciences, 2007, 18(3): 338-345.)
［43］Jackson R J, Standart N. Do the poly (A) tail and 3′ untranslated region control mRNA translation［J］. Cell, 1990, 62(1): 15-24.
［44］云涛. DHVⅠ感染性分子克隆的建立及其衍生病毒的生物学特性研究［D］. 杨凌: 西北农林科技大学, 2010. (Yun T. Development and characterizations of an infect-ious molecular clone and its derivatives of duck hepatitis virus type I［D］. Yangling: North West Agricultural and Forestry University, 2010.)
［45］Kekarainen T, Savilahti H, Valkonen J P T. Functional genomics on potato virus A: Virus genome-wide map of sites essential for virus propagation［J］. Genome Research, 2002, 12(4): 584-594.
［46］Shih D S, Dasgupta R, Kaesberg P 7Methyl-guanosine and efficiency of RNA translation［J］. Journal of Virology, 1976, 19(2): 637-642.
［47］Kato S, Ohtoko K, Ohtake H, et al.Vector-Capping: A simple method for preparing a high-quality ful-length cDNA library［J］. DNA Reserch, 2005, 12(1): 53-62.
［48］刘宁, 刘若淼, 马莹, 等. 中国大豆花叶病毒HCPro基因的克隆与序列分析［J］. 大豆科学, 2010, 29(4): 549-554. (Liu N, Liu R M, Ma Y, et al. Cloning and sequence analysis of HCPro gene of soybean mosaic virus［J］. Soybean Science, 2010, 29(4): 549-554.
［49］王大刚, 智海剑, 田震, 等. 大豆花叶病毒致病基因的克隆与序列分析［J］. 大豆科学, 2015, 34(5): 760-767.(Wang D G, Zhi H J, Tian Z, et al.Clone and sequence analysis of virulence genes of soybean mosaic virus isolates in China［J］. Soybean Science, 2015, 34(5): 760-767.)
［50］Mai T L T, Tung P T, Son L V, et al. Construction of vector carrying RNAi structures containing CP gene fragment from SMV and BYMV［J］. Journal of Biology, 2014, 35(3se): 129-135.
［51］刘江, 陈沛, 邢光南,等. 大豆查尔酮还原酶基因GmCHR的克隆与植物表达载体构建［J］. 大豆科学, 2013, 32(2): 139-142. (Liu J, Chen P, Xing G N, et al. Cloning of the soybean chalcone reductase gene GmCHR and construction of its plant expression vector［J］. Soybean Science, 2013, 32(2): 139-142.
［52］王彬, 徐志华, 侯金锋, 等. 大豆细胞质转化酶基因GmCInv的克隆与植物表达载体构建［J］. 大豆科学, 2014, 33(2): 168-172. (Wang B, Xu Z H, Hou J F, et al. Cloning of soybean cytoplasmic invertase gene GmCInv and construction of its plant expression vector［J］. Soybean Science, 2014, 33(2): 168-172.
［53］Liu C, Yang B, Ming Y, et al. Construction of an RNAi vector for knockdown of GM-ACS genes in the cotyledonary nodes of soybean［J］. Electronic Journal of Biotechnology, 2017, 26: 40-45.
［54］王永志, 苏颖, 米丽娟, 等. 东北地区大豆花叶病毒3 号株系衣壳蛋白的表达、纯化及单克隆抗体制备［J］. 吉林农业科学, 2011, 36（6）：37-39. (Wang Y Z, Su Y, Mi L J, et al. Expression, purification and monoclonal antibody preparation of northeastern region SMV CP protein［J］. Journal of Jilin Agricultural Sciences, 2011, 36(6): 37-39.)
［55］张淋淋, 李小宇, 张春雨, 等. 大豆花叶病毒东北3号株系NIb蛋白表达、纯化及单克隆抗体制备［J］. 东北农业科学, 2016, 41(3): 62-66. (Zhang L L, Li X Y, Zhang C Y, et al. Expression and purification of a NIb protein of the SMV strain 3 from the no-rtheastern regions of China and preparation of its monoclonal antibody［J］. Journal of Northeast Agricultural Sciences, 2016, 41(3): 62-66.)
［56］Zhu M, Zhang W, Tian J, et al. Development of a lateral-flow assay(LFA) for rapid detection of soybean mosaic virus［J］. Journal of Virological Methods, 2016, 235: 51-57.
［57］王大刚, 田震, 李凯, 等. 鲁豫皖大豆产区大豆花叶病毒株系的鉴定及动态变化分析［J］.大豆科学, 2013, 32(6): 806-809.(Wang D G, Tian Z, Li K, et al. Identification and variation analysis of soybean mosaic virus strains in Shandong, Henan and Anhui provinces of China［J］. Soybean Science, 2013, 32(6): 806-809.)
［58］章洁琼, 李红艳, 胡小南, 等. 农杆菌介导的RNAi CP基因在大豆中的转化［J］.作物学报, 2013, 39(9): 1594-1601. (Zhang J Q, Li H Y, Hu X N, et al. Agrobacterium tumefaciens mediated transformation of RNAi CP gene into soybean (Glycine max L)［J］. Acta Agronomica Sinica, 2013, 39(9): 1594-1601.)

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[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(05):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(05):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(05):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(05):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]李 凯,刘志涛,李海朝,等.国家大豆区域试验品种对SMV和SCN的抗性分析[J].大豆科学,2013,32(05):670.[doi:10.11861/j.issn.1000-9841.2013.05.0670]
　LI Kai,LIU Zhi-tao,LI Hai-chao,et al.Resistance to Soybean Mosaic Virus and Soybean Cyst Nematode of Soybean Cultivars from China National Soybean Uniform Trials[J].Soybean Science,2013,32(05):670.[doi:10.11861/j.issn.1000-9841.2013.05.0670]
[12]王大刚,田 震,李 凯,等.鲁豫皖大豆产区大豆花叶病毒株系的鉴定及动态变化分析[J].大豆科学,2013,32(06):806.[doi:10.11861/j.issn.1000-9841.2013.06.0806]
[13]张雯娜,李晋玉,田金艳,等.逆转录环介导等温扩增技术快速检测大豆花叶病毒[J].大豆科学,2014,33(03):422.[doi:10.11861/j.issn.10009841.2014.03.0422]
　ZHANG Wen-na,LI Jin-yu,TIAN Jin-yan,et al.Rapid Detection of Soybean Mosaic Virus by Reverse Transcription Loop Mediated Isothermal Amplification[J].Soybean Science,2014,33(05):422.[doi:10.11861/j.issn.10009841.2014.03.0422]
[14]郭东全,王延伟,智海剑,等.大豆对SMV SC-13株系群的抗性遗传及基因定位的研究[J].大豆科学,2007,26(01):21.[doi:10.3969/j.issn.1000-9841.2007.01.006]
　GUO Dong-quan,WANG Yan-wei,ZHI Hai-jian,et al.INHERITANCE AND GENE MAPPING OF RESISTANCE TO SMV STRAIN GROUP SC-13 IN SOYBEAN[J].Soybean Science,2007,26(05):21.[doi:10.3969/j.issn.1000-9841.2007.01.006]
[15]李凯,夏迎春,王大刚,等.黑龙江省大豆花叶病毒（SMV）株系的动态变化分析[J].大豆科学,2014,33(06):880.[doi:10.11861/j.issn.1000-9841.2014.06.0880]
　LI Kai,XIA Ying-chun,WANG Da-gang,et al.Analysis of Dynamic Change of Soybean Mosaic Virus Strains in Heilongjiang Province of China[J].Soybean Science,2014,33(05):880.[doi:10.11861/j.issn.1000-9841.2014.06.0880]
[16]张锴,等.大豆花叶病毒引发大豆症状类型的研究[J].大豆科学,2015,34(06):1011.[doi:10.11861/j.issn.1000-9841.2015.06.1011]
　ZHANG Kai,REN Rui,et al.The Symptoms Types in Soybean Leaves Caused by Soybean Mosaic Virus[J].Soybean Science,2015,34(05):1011.[doi:10.11861/j.issn.1000-9841.2015.06.1011]
[17]宋英培,高乐,刘志涛,等.大豆花叶病毒成株抗性及种粒抗性的鉴定[J].大豆科学,2015,34(06):1015.[doi:10.11861/j.issn.1000-9841.2015.06.1015]
　SONG Ying-pei,GAO Le,LIU Zhi-tao,et al.Evaluation of Adult Resistance and Soybean Seed Resistance to Soybean Mosaic Virus[J].Soybean Science,2015,34(05):1015.[doi:10.11861/j.issn.1000-9841.2015.06.1015]
[18]刘丽君,吴俊江,高明杰,等.SMV侵染对大豆内源激素平衡的影响[J].大豆科学,2000,19(04):326.[doi:10.11861/j.issn.1000-9841.2000.04.0326]
　[J].Soybean Science,2000,19(05):326.[doi:10.11861/j.issn.1000-9841.2000.04.0326]
[19]黄志平,李杰坤,王维虎,等.大豆新品系抗SMV鉴定及其抗性来源分析[J].大豆科学,2017,36(04):598.[doi:10.11861/j.issn.1000-9841.2017.04.0598]
　HUANG Zhi-ping,LI Jie-kun,WANG Wei-hu,et al.Identification of Resistance and Preliminary Analysis of Resistance Sources for the Soybean Mosaic Virus in New Soybean Lines[J].Soybean Science,2017,36(05):598.[doi:10.11861/j.issn.1000-9841.2017.04.0598]
[20]王大刚,陈圣男,李杰坤,等.大豆品系抗SMV评价及亲本来源分析[J].大豆科学,2018,37(05):657.[doi:10.11861/j.issn.1000-9841.2018.05.0657]
　WANG Da-gang,CHEN Sheng-nan,LI Jie-kun,et al.Lines to Soybean Mosaic Virus[J].Soybean Science,2018,37(05):657.[doi:10.11861/j.issn.1000-9841.2018.05.0657]

备注/Memo

基金项目：东北农业大学大学生创新基金（201610224147）；哈尔滨市应用技术研究与开发项目（2014AB6BN036）；中央引导地方科技发展专项（ZY16C06）。

第一作者简介：张恩柱（1995-），男，学士，主要从事寄主与病原物互作研究。E-mail：zhang_enzhu@163.com。
通讯作者：张俊华（1973-），男，教授，博导，主要从事寄主与病原物互作研究。E-mail：podozjh@163.com。

更新日期/Last Update: 2017-11-01