[1]宁海龙,庄煦,杨畅,等.大豆四向重组自交系群体生育进程稳定QTL定位[J].大豆科学,2017,36(05):692-698.[doi:10.11861/j.issn.1000-9841.2017.05.0692]
　NING Hai-long,ZHUANG Xu,YANG Chang,et al.Mapping Stable QTLs for Growth Period Procedure Based on Four-way Recombination Inbred Line Population[J].Soybean Science,2017,36(05):692-698.[doi:10.11861/j.issn.1000-9841.2017.05.0692]
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大豆四向重组自交系群体生育进程稳定QTL定位

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年05期 页码: 692-698 栏目: 出版日期: 2017-09-20
Title:
Mapping Stable QTLs for Growth Period Procedure Based on Four-way Recombination Inbred Line Population
作者:
宁海龙1; 庄煦1; 杨畅1; 方艳龙1; 王萍1; 2; 李文滨1; 薛红1; 李文霞1
（1.东北农业大学 农学院/大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室，黑龙江 哈尔滨 150030； 2.黑龙江省农业科学院信息中心，黑龙江 哈尔滨 150086）
Author(s):
NING Hai-long1;  ZHUANG Xu1;  YANG Chang1;  FANG Yan-long1;  WANG Ping1; 2;  LI Wen-bin1;  XUE Hong1;  LI Wen-xia1
(1.Agronomy Colledge, Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education/Key Laboratory of Soybean Biology and Breeding（Genetics）, Ministry of Agriculture, Harbin 150030, China; 2.Information Center of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China)
关键词:
大豆; 生育进程; 四向重组自交系群体; QTL
Keywords:
Soybean;  Growth process;  Four-way recombination inbred line population;  QTL
DOI:
10.11861/j.issn.1000-9841.2017.05.0692
文献标志码:
A
摘要:
以垦丰14、垦丰15、垦丰19和黑农48为亲本，构建了含160个株系的四向重组自交系群体（four-way recombinant inbred lines，FW-RIL）为试验材料，通过构建分子遗传连锁图谱，对大豆四向重组自交系群体生育期各阶段进行QTL定位，旨在为大豆生育期性状的分子辅助育种提供理论基础和技术支撑，在分子水平探索与大豆生育期相关基因的遗传机理。通过区间作图法等分析方法，应用275个SSR分子标记对2013、2014与2015年在克山和哈尔滨3年3个环境下的生育期各阶段进行QTL定位，结果表明：大豆四向杂交群体的各生育期阶段易受到环境条件的影响，生育期相关性状在Fw-RIL群体中存在真实的遗传变异。76个与生育期各阶段相关的QTL分布在18个连锁群上，分别是A1、A2、B1、B2、C1、C2、D1a、D1b、D2、F、G、H、J、K、L、M、N和O连锁群；17个与生育期各阶段相关的QTL能够在2个以上环境中被重复定位到，分别为qST-B2-1、qST-C1-1、qST-C2-1、qST-C2-2、qST-D1b-1、qST-D1b-2、qST-G-1、qST-H-1、qST-K-1、qST-K-2、qST-L-1、qST-L-2、qST-M-1、qST-M-2、qST-M-3、qST-N-1、qST-O-1。其中，新发现10个与生育期相关的QTLs,qST-C2-2,qST-D1b-1,qST-k1,qST-M2和qST-N1是高效QTL。
Abstract:
In this study, fourway recombinant inbred lines were constructed with Kenfeng 14, Kenfeng 15, Kenfeng 19 and Heinong 48 as the parents.The aim was to provide a theoretical basis and technical support for the molecular-assisted breeding of soybean growth stages, and to explore the genetic mechanism of genes related to growth period.QTL mapping was carried out at different stages of growth period in 2013, 2014 and 2015 under the three environments of Keshan and Harbin by 275 SSR molecular markers. The results showed that the growth stages of soybean four-way recombinant inbred lines were susceptible to environmental conditions, and the growth traits of the four-way RIL population had significant genetic variation.The QTLs associated with each stage of the growth period were distributed on 18 linkage groups, respectively, A1, A2, B1, B2, C1, C2, D1a, D1b, D2, F, G, H, J, K, L, M, N and O linkage groups;17 QTLs associated with each stage of the growth period were repeatedly mapped, respectively, qST-B2-1, qST-C1-1, qST-C2-1, qST-C2-2, qST-D1b-1, qST-D1b-2, qST-G-1, qST-H-1, qST-K-1, qST-K-2, qST-L-1, qST-L-2, qST-M-1, qST-M-2, qST-M-3, qST-N-1 and qST-O-1. Among them, 10 QTLs loci were newly found, qST-C2-2, qST-D1b-1, qST-K-1, qST-M-2 and qST-N-1 were localized in three or more environment or stages of growth period.

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备注/Memo

基金项目：黑龙江省自然科学基金面上项目（C2015007）；黑龙江省留学归国人员科学基金项目（LC2016010）；黑龙江省教育厅科学技术研究重点项目（12541z001）；黑龙江省博士后科研启动基金（LBH-Q12152）；黑龙江省博士后科研启动基金（LBH-Q09165）。

第一作者简介：宁海龙(1975-)，男，教授，博导，主要从事作物遗传育种与数量遗传研究。E-mail: ninghailongneau@126.com。
通讯作者：李文霞(1974-)，女，副教授，硕导，主要从事作物遗传育种研究。E-mail: liwenxianeau@126.com。

更新日期/Last Update: 2017-10-29