[1]黄志平,李杰坤,王维虎,等.大豆新品系抗SMV鉴定及其抗性来源分析[J].大豆科学,2017,36(04):598-605.[doi:10.11861/j.issn.1000-9841.2017.04.0598]
　HUANG Zhi-ping,LI Jie-kun,WANG Wei-hu,et al.Identification of Resistance and Preliminary Analysis of Resistance Sources for the Soybean Mosaic Virus in New Soybean Lines[J].Soybean Science,2017,36(04):598-605.[doi:10.11861/j.issn.1000-9841.2017.04.0598]
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大豆新品系抗SMV鉴定及其抗性来源分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年04期 页码: 598-605 栏目: 出版日期: 2017-07-20
Title:
Identification of Resistance and Preliminary Analysis of Resistance Sources for the Soybean Mosaic Virus in New Soybean Lines
作者:
黄志平; 李杰坤; 王维虎; 胡国玉; 吴倩; 王大刚
（安徽省农业科学院 作物研究所/安徽省农作物品质改良重点实验室，安徽 合肥 230031）
Author(s):
HUANG Zhi-ping;  LI Jie-kun;  WANG Wei-hu;  HU Guo-yu;  WU Qian;  WANG Da-gang
(Crop Institute of Anhui Academy of Agricultural Sciences/Key Laboratory of Crop Quality Improvement of Anhui Province, Hefei 230031,China)
关键词:
大豆; 大豆花叶病毒; 抗性鉴定; 抗性来源
Keywords:
Soybean;  Soybean mosaic virus;  Identification;  Resistance sources
DOI:
10.11861/j.issn.1000-9841.2017.04.0598
文献标志码:
A
摘要:
大豆花叶病毒（soybean mosaic virus, SMV）病是危害我国大豆生产的主要病害之一。利用黄淮大豆产区的SMV优势株系SC3和SC7对选育的394份大豆高世代新品系进行抗病性评价并分析其抗性来源。结果表明：对SC3株系表现高抗的品系有120份，占试验品系总数的30.46%；高抗SC7株系的品系有80份，占20.30%；对SC3和SC7株系都表现高抗的品系有64份，占鉴定品系数的16.24%。如大豆新品系H20443、H21660、H22501、Y50574和Y52933等通过审定后用于大田的生产将对SMV的流行起到控制作用。对选育的大豆新品系进行抗性来源分析可以发现，RT（抗病型）×RT组合获得抗病型后代品系的概率最高，其后依次为RT×IT（中间型） >RT×ST（感病型） >IT×RT >IT×IT >ST×RT >ST×IT，后代品系出现抗病型概率最低的组合是ST×ST。这些结果可为大豆新品系的选育和抗病育种亲本的组配提供参考依据。
Abstract:
Soybean mosaic virus disease is one of main diseases reducing quality and yield in soybean ［Glycine max (L) Merr］. The resistance of 394 new bred soybean lines was tested using soybean mosaic virus (SMV) prevalent strains SC3 and SC7. The resistance sources were carried out through investigating the parental resistance. The results showed that among the 394 lines inoculated by SC3 and SC7, 120 (30.46%) and 80 (20.30%) exhibited high resistance, respectively.64 lines (16.24%), such as H20443, H21660, H22501, Y50574 and Y52933 et al. were high resistant to both SC3 and SC7, and should be utilized as resistance resources in breeding programs.The analysis of the resistance sources of new lines showed that the offspring of resistant type (RT)×RT was the highest probability with the resistance new lines, and others successively were the offspring of RT×intermediate type (IT) >RT×susceptible type (ST) >IT×RT >IT×IT >ST×RT >ST×IT, the offspring of ST×ST was the lowest probability with the resistance new lines.These results will provide references for the breeding of new soybean lines and the combination of resistance breeding parents.

参考文献/References:

［1］李凯，刘志涛，李海朝，等．国家大豆区域试验品种对SMV和SCN的抗性分析［J］．大豆科学，2013，32（5）：670-675．(Li K, Liu Z T, Li H C, et al.Resistance to soybean mosaic virus and soybean cyst nematode of soybean cultivars from China national soybean uniform trials ［J］. Soybean Science, 2013, 32(5): 670-675.)

［2］智海剑，盖钧镒，陈应志，等．2002-2004年国家大豆区试品种对大豆花叶病毒抗性的评价［J］．大豆科学，2005，24（3）：190-192．(Zhi H J, Gai J Y, Chen Y Z, et al. Evaluation of resistance to SMV of the entries in the national uniform soybean tests (2002-2004) ［J］. Soybean Science, 2005, 24(3): 190-192.)
［3］白丽，李凯，陈应志，等．部分国家和省（市）区试品种对大豆花叶病毒的抗性分析［J］．中国油料作物学报，2007，29（1）：86-89．(Bai L, Li K, Chen Y Z, et al. Evaluation of resistance to SMV of cultivars from soybean nation and local regional test ［J］. Chinese Journal of Oil Crop Sciences, 2007, 29(1): 86-89.)
［4］杨华，李凯，杨清华，等．国内部分新品种对大豆花叶病毒抗性的鉴定［J］．华北农学报，2008，23（S2）：253-255．(Yang H, Li K, Yang Q H ,et al.Evaluation of resistance to SMV of cultivars from soybean national and local test in 2004-2006 ［J］. Acta Agriculturae Boreali-Sinica, 2008, 23(S2): 253-255.)
［5］王大刚，卢为国，马莹，等．新育成大豆品种对SMV和SCN的抗性评价［J］．大豆科学，2009，28（6）：949-953．(Wang D G, Lu W G, Ma Y, et al. Evaluation of resistance of soybean cultivars to soybean mosaic virus and soybean cyst nematode ［J］. Soybean Science, 2009, 28(6): 949-953.)
［6］王修强，盖钧镒，濮祖芹．黄淮和长江中下游地区大豆花叶病毒株系鉴定与分布［J］．大豆科学，2003，22（2）：102-107-(Wang X Q, Gai J Y, Pu Z Q. Classification and distribution of strain groups of soybean mosaic virus in middle and lower Huang-Huai and Changjiang valleys ［J］. Soybean Science, 2003, 22(2): 102-107.)
［7］智海剑，盖钧镒，何小红．大豆对SMV数量（程度）抗性的综合分级方法研究［J］．大豆科学，2005，24（2）：5-11．(Zhi H J, Gai J Y, He X H. Study on methods of classification of quantitative resistance to soybean mosaic virus in soybean ［J］. Soybean Science, 2005, 24(2): 5-11.)
［8］王大刚，黄志平，张磊，等．大豆抗大豆花叶病毒病鉴定技术规程［S］．安徽省质量技术监督局，DB34/T 2510-2015．(Wang D G, Huang Z P, Zhang L, et al. Rules for evaluation of soybean for resistance to soybean mosaic virus ［S］. Anhui Bureau of Quality and Technical Supervision, DB34/T 2510-2015)
［9］Zhi H J, Gai J Y. Performances and germplasm evaluation of quantitative resistance to soybean mosaic virus in soybeans ［J］. Agricultural Science in China, 2004, 3(4): 247-253.
［10］侯文焕，杨永庆，林静，等．不同来源大豆材料对SMV株系SC3和SC7的抗性分析［J］．大豆科学，2015，34（5）：861-866．(Hou W H, Yang Y Q, Lin J, et al.Resistance of different origin soybeans to SMV strains SC3 and SC7 ［J］. Soybean Science, 2015, 34(5): 861-866.)
［11］智海剑，盖钧镒．大豆对SMV数量抗性的表现形式与种质鉴定［J］．中国农业科学，2004，37（10）：1422-1427．(Zhi H J, Gai J Y. Performances and germplasm evaluayion of quantitative resistance to soybean mosaic virus in soybeans ［J］. Chinese Agricultural Sciences, 2004, 37(10): 1422-1427.)
［12］王大刚，胡国玉，黄志平，等．大豆种质资源抗大豆花叶病毒的鉴定［J］．作物杂志，2013(2)：32-36．(Wang D G, Hu G Y, Huang Z P, et al. Identification of soybean germplasm resources resistance to soybean mosaic virus ［J］. Crops, 2013(2): 32-36.)
［13］于国宜，王大刚，吴倩，等．2009-2015年安徽省大豆试验新品系对SMV和SCN的抗性评价［J］．大豆科学，2016，35（5）：782-788．(Yu G Y, Wang D G, Wu Q, et al.Evaluation of resistance of soybean new lines to soybean mosaic virus and soybean cyst nematode in Anhui Province in 2009-2015.［J］. Soybean Science, 2016, 35(5): 782-788.)
［14］黄志平，王维虎，张磊，等．分子标记辅助黄淮大豆生育期组归属研究［J］．中国油料作物学报，2016，38（6）：713-721．(Huang Z P, Wang W H, Zhang L, et al. Maturity group classification of soybean varieties with molecular marker in Huang-Huai region ［J］. Chinese Journal of Oil Crop Sciences, 2016, 38(6): 713-721.)
［15］毕研飞，徐兵划，钱春桃，等．分子标记辅助甜瓜抗蔓枯病基因的聚合及品种改良［J］．中国农业科学，2015，48（3）：523-533．(Bi Y F, Xu B H, Qian C Y, et al. Pyramiding disease resistance genes and variety improvement by molecular marker-assisted selection in melon (Cucumis melon L) ［J］. Chinese Agricultural Sciences, 2015, 48(3): 523-533.)
［16］Ma Y, Li H C, Wang D G, et al. Molecular mapping and marker assisted selection of resistance gene RSC12 to soybean mosaic virus in soybean ［J］. Legume Genomics and Genetics, 2010, 1(8): 41-46.
［17］韩英鹏，赵雪，李修平，等．大豆种质对花叶病毒病和疫霉根腐病抗病性的SSR标记辅助鉴定［J］．大豆科学，2014，33（1）：27-30．(Han Y P, Zhao X, Li X P, et al. SSR identification of soybean cultivar with resistance to soybean mosaic virus and Phytophthpra root rot ［J］. Soybean Science, 2014, 33(1): 27-30).

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　LI Kai,LIU Zhi-tao,LI Hai-chao,et al.Resistance to Soybean Mosaic Virus and Soybean Cyst Nematode of Soybean Cultivars from China National Soybean Uniform Trials[J].Soybean Science,2013,32(04):670.[doi:10.11861/j.issn.1000-9841.2013.05.0670]
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　ZHANG Wen-na,LI Jin-yu,TIAN Jin-yan,et al.Rapid Detection of Soybean Mosaic Virus by Reverse Transcription Loop Mediated Isothermal Amplification[J].Soybean Science,2014,33(04):422.[doi:10.11861/j.issn.10009841.2014.03.0422]
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　[J].Soybean Science,2000,19(04):326.[doi:10.11861/j.issn.1000-9841.2000.04.0326]
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　ZHANG En-zhu,ZHAO Wei,ZHANG Lin-lin,et al.Advances in Infectious Cloning of Soybean Mosaic Virus[J].Soybean Science,2017,36(04):808.[doi:10.11861/j.issn.1000-9841.2017.05.0808]
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备注/Memo

基金项目：国家自然科学基金（31571687）；安徽省自然科学基金（1708085MC69）；国家大豆产业技术体系建设专项资金（CARS-04）。

第一作者简介：黄志平（1969-），男，研究员，主要从事大豆遗传育种研究。E-mail: hzhpsoy@163.com。
通讯作者：王大刚（1979-），男，副研究员，主要从事大豆抗病遗传育种研究。E-mail: smvwang@163.com。

更新日期/Last Update: 2017-08-14