[1]姚敏磊,张璟曜,周汐,等.大豆响应低磷胁迫的数字基因表达谱分析[J].大豆科学,2016,35(02):213-221.[doi:10.11861/j.issn.1000-9841.2016.02.0213]
　YAO Min-lei,ZHANG Jing-yao,ZHOU Xi,et al.The Digital Gene Expression Profiling Analysis of Genes in Response to Low Phosphorus Stress in Soybean[J].Soybean Science,2016,35(02):213-221.[doi:10.11861/j.issn.1000-9841.2016.02.0213]
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大豆响应低磷胁迫的数字基因表达谱分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年02期 页码: 213-221 栏目: 出版日期: 2016-03-20
Title:
The Digital Gene Expression Profiling Analysis of Genes in Response to Low Phosphorus Stress in Soybean
作者:
姚敏磊1; 张璟曜1; 周汐1; 韩少怀1; 谢凤斌1; 朱月林1; 2; 盖钧镒1; 杨守萍1
1.南京农业大学 大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室(综合)/作物遗传与种质创新国家重点实验室,江苏 南京 210095；
2.南京农业大学 园艺学院,江苏 南京 210095
Author(s):
YAO Min-lei1;  ZHANG Jing-yao1;  ZHOU Xi1;  HAN Shao-huai1;  XIE Feng-bin1;  ZHU Yue-lin1; 2;  GAI Jun-yi1;  YANG Shou-ping1
1.Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/Key Laboratory of Biology and Genetic Improvement of Soybean(General)， Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement，Nanjing Agricultural University, Nanjing 210095，China；
2.College of Horticulture，Nanjing Agricultural University，Nanjing 210095，China
关键词:
大豆; 低磷胁迫; 数字基因表达谱; 差异表达基因
Keywords:
Soybean; Low phosphorus stress; Digital gene expression profiling; Differential expressed genes
DOI:
10.11861/j.issn.1000-9841.2016.02.0213
文献标志码:
A
摘要:
以OsPT6转基因T5代大豆为材料，以其受体亲本东农50为对照，对低磷胁迫处理下的根系cDNA文库进行数字基因表达谱分析，共筛选得到33个差异表达基因。以受体亲本为对照，在OsPT6转基因大豆中上调表达的差异基因有21个、下调表达的差异基因有12个。GO功能显著性富集分析表明，有25个差异表达基因在蛋白、核酸等生物大分子代谢、次生代谢和酶活性调节等过程中表现为富集。KEGG代谢通路分析表明仅有1个过氧化物酶超蛋白家族基因参与到苯丙合成、苯丙氨酸代谢和次生代谢产物合成等次生代谢途径中。综合分析表明：通过光合作用相关酶类活性的调节控制光合作用速率从而影响次生代谢途径可能是大豆适应低磷胁迫的主要途径。以上结果为大豆响应低磷胁迫相关分子机制的深入研究和功能基因的筛选奠定了基础。
Abstract:
In this study，the T5?line of the soybean cultivar transferred with OsPT6 gene and its receptor parent Dongnong 50 under low phosphorus stress were used to analyze their root cDNA library by digital gene expression profiling technology．The results showed that a total of 33 differential expressed genes(DEGs)were screened．Compared with the receptor parent, 21 DEGs were upregulated and 12 DEGs were down-regulated in the OsPT6 transgenic soybean．GO enrichment analysis showed that 25 DEGs were enriched in the processes of biological macromolecules metabolism, secondary metabolism and enzyme activity regulation． The KEGG pathway analysis showed that only one peroxidase superfamily protein was involved in the pathways such as phenylpropanoid biosynthesis, phenylalanine metabolism and biosynthesis of secondary metabolites． Comprehensive analysis showed that the photosynthesis rate affected the secondary metabolic pathways by regulating activity of the enzymes involved in photosynthesis，which could be the main way of soybean adapted to low phosphorus stress．These results provided a base for further study on the molecular mechanism in response to low phosphorus stress and functional genes screening in soybean．

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备注/Memo

基金项目：国家农作物转基因重大专项(2011ZX08004-005，2013ZX08004005，2014ZX08004-005)；国家高技术研究发展计划“863计划”(2011AA10A105)；国家重点基础研究发展计划“973计划”(2011CB109301)；教育部长江学者和创新团队发展计划(PCSIRT13073)；江苏省现代作物生产协同创新中心(JCIC-MCP)。

第一作者简介：姚敏磊(1991-)，男，硕士，主要从事大豆遗传育种研究。E-mail:yaominlei1991@sina.com。
通讯作者：杨守萍(1967-)，女，博士，教授，主要从事大豆遗传育种研究。E-mail:spyung@126.com。

更新日期/Last Update: 2016-04-04