[1]于志晶,尚丽霞,蔡勤安,等.水稻热激蛋白基因HSP90转化大豆的研究[J].大豆科学,2016,35(02):222-227.[doi:10.11861/j.issn.1000-9841.2016.02.0222]
　YU Zhi-jing,SHANG Li-xia,CAI Qin-an,et al.Transformation of Heat Shock Protein Gene HSP90 of Rice into Soybean[J].Soybean Science,2016,35(02):222-227.[doi:10.11861/j.issn.1000-9841.2016.02.0222]
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水稻热激蛋白基因HSP90转化大豆的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年02期 页码: 222-227 栏目: 出版日期: 2016-03-20
Title:
Transformation of Heat Shock Protein Gene HSP90 of Rice into Soybean
作者:
于志晶1;  尚丽霞1;  蔡勤安1;  孟凡钢2 ; 马瑞1
1.吉林省农业科学院 农业生物技术研究所，吉林 长春 130033；
2.吉林省农业科学院 大豆研究所，吉林 长春 130033
Author(s):
YU Zhi-jing1;  SHANG Li-xia1; CAI Qin-an1; MENG Fan-gang2; MA Rui1
1.Agro-Biotechnology Research Institute，Jilin Academy of Agricultural Sciences，Changchun 130033，China；
2.Soybean Research Institute，Jilin Academy of Agricultural Sciences，Changchun 130033，China
关键词:
大豆; 耐旱; 热激蛋白; 遗传转化
Keywords:
Soybean; Drought tolerance; Heat shock protein; Genetic transformation
DOI:
10.11861/j.issn.1000-9841.2016.02.0222
文献标志码:
A
摘要:
由于干旱和盐碱化的严重影响，我国大豆生产受到很大限制。为了提高大豆的抗旱性，培育抗旱转基因大豆新品种，利用农杆菌介导的子叶节遗传转化技术体系，首次将水稻热激蛋白基因HSP90导入大豆受体材料Bert中，通过HSP90在大豆中过表达，获得了耐旱转基因大豆新材料。本试验中4 000个子叶节外植体用于遗传转化，再生转化苗经PCR和Southern杂交鉴定结合PPT抗性筛选（bar为筛选标记），共获得128棵阳性转基因植株,转化率为3.2%。经初步筛选，获得15份耐旱性较好的材料，其耐旱性显著优于对照。研究结果为进一步筛选耐旱转基因大豆新材料奠定了较好的基础。
Abstract:
Soybean is an important food and oil crop in China, and is vital to the national economy.However, the soybean production is limited by drought and salinization.In order to breed the transgenic soybean variety with drought tolerance, the heat shock protein gene .HSP90 from rice was first transformed into soybean mediated with Agrobacterium tumefaciens in this study. The transgenic plants with tolerance to drought can be further selected through over-expression HSP90 gene driven by TMV35S promoter. In this experiment total 4 000 cotyledonary node explants infected and 128 independent transgenic plants were obtained by PCR and Southern blot analysis combining PPT selection. The transformation efficiency was 3.2%. Fifteen transgenic plants with drought tolerance were selected.The transgenic plants obtained in this study would be important for further drought tolerance breeding of soybean.

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备注/Memo

基金项目：吉林省科技厅重点科技攻关项目（20130206005NY）；农业部转基因生物新品种培育重大专项（2014ZX08004-002-001）。

第一作者简介：于志晶(1977-)，女，硕士，主要从事植物分子生物学、遗传转化与代谢工程研究。E-mail：yuzhijing0001@163.com。
通讯作者：孟凡钢(1978-)，男，硕士，副研究员，主要从事大豆遗传育种研究。E-mail：mengfg2013@163.com；马瑞（1966 -），男，博士研究员，主要从事植物生物技术研究。E-mail：ruimaa@126.com。

更新日期/Last Update: 2016-04-04