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[1]孟永杰,陈锋,帅海威,等.大豆EMS突变体库构建及其M1代重要农艺性状评价[J].大豆科学,2016,35(01):64-69.[doi:10.11861/j.issn.1000-9841.2016.01.0064]
　MENG Yong-jie,CHEN Feng,SHUAI Hai-wei,et al.Construction of EMS Mutant Library and the Evaluation of Important Agronomic Traits of M1?in Glycine max[J].Soybean Science,2016,35(01):64-69.[doi:10.11861/j.issn.1000-9841.2016.01.0064]

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大豆EMS突变体库构建及其M₁代重要农艺性状评价

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷期数: 2016年01期页码: 64-69 栏目: 出版日期: 2016-01-20

Title:: Construction of EMS Mutant Library and the Evaluation of Important Agronomic Traits of M₁?in Glycine max

作者:: 孟永杰; 陈锋; 帅海威; 刘卫国; 杜俊波; 刘江; 舒凯; 杨文钰; 四川农业大学农学院/农业部西南作物生理生态与耕作重点实验室，四川成都 611130

Author(s):: MENG Yong-jie; CHEN Feng; SHUAI Hai-wei; LIU Wei-guo; DU Jun-bo; LIU Jiang; SHU Kai; YANG Wen-yu; Agronomy College of Sichuan Agricultural University/Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of China, Chengdu 611130, China

关键词:: 大豆; EMS; 突变体库; 农艺性状

Keywords:: Keywords: Soybean; EMS; Mutant pool; Agronomic traits

DOI:: 10.11861/j.issn.1000-9841.2016.01.0064

文献标志码:: A

摘要:: 摘要：构建突变体库是进行遗传学与分子生物学研究的重要前提条件之一。当前大豆突变体数量的不足，严重制约了大豆功能基因组学研究的深入开展。以南方大面积推广的大豆品种-南豆12为材料，通过EMS诱变构建了大豆EMS突变体库。统计发现，M₁种子田间出苗率为34.85%，表明EMS强烈抑制大豆种子的萌发和出苗。对随机选取的913株M₁植株进行了株高、单株荚数等重要农艺性状的调查。结果表明：经EMS处理后，M₁植株在株高、单株荚数、节间数等方面与对照存在显著差异、表型变异丰富，其中18.07%发生矮化，11.06%表现出多荚，另有3株不育株；群体间的节间个数极差达到18。这些数据表明已成功构建了大豆EMS突变体库。在进一步的研究中，将对单个突变体进行分离、分类，并克隆相应的重要功能基因，为深入研究大豆功能基因组学以及筛选适合玉米-大豆带状复合种植模式的高光效、抗倒伏大豆品种奠定基础。

Abstract:: Sufficient mutant pool is one of the most important preconditions for molecular biology and plant genetics studies.Lacking of enough mutants of soybean is a serious constraint to soybean functional genomics researching.In this study, soybean mutant pool was constructed by treating seeds of Nandou 12, which is prevailed in Southwestern China, by Ethyl Methyl Sulfone (EMS) treatment.The results showed that, emergence rate of M₁?seeds in field barely reached to 34.85%, which indicated that EMS significantly inhibit the emergence of soybean seeds. Analysis of the important agronomic traits, including plant height, pods per plant and so on, of 913 plants of M₁?generation showed that, plant height, pods per plant and internodes number were significantly different with Nandou 12.Compared to Nandou 12, 18.07% of the pool showed the dwarf phenotype; 11.06% revealed more pods per plant, and 3 sterile mutants were observed.Furthermore, the range (R) of internodes number of M₁?lines reached to 18.Taken together, these results demonstrated that soybean mutant pool had been constructed primarily. Classification of single mutant and cloning of the corresponded gene(s) would be focused on the following studies, which would has a profound effect on soybean functional genetics study and screening perfect soybean varieties for maize-soybean stripe intercropping system.

参考文献/References:

［1］Lino M. Toward understanding the ecological functions of tropisms: Interactions among and effects of light on tropisms ［J］. Current Opinion in Plant Biology, 2006, 9(1): 89-93.

［2］Velculescu V E, Zhang L, Vogelstein B, et al.Serial analysis of gene expression［J］ Science, 1995, 270(5235): 484-486.

［3］代君丽，李振岐.小麦的比较基因组学和功能基因组学［J］. 西北植物学报, 2004, 24(5): 949-953. (Dai J L, Li Z Q. Comparative and functional genomics of wheat［J］. Acta Botanica BorealiOccidentalia Sinica, 2004, 24(5): 949-953.)［4］Somerville C, Somerville S. Plant functional genomics ［J］. Science, 1999, 285(5426): 380-383

［5］Bouchez D, Hofte H. Functional genomics in plants ［J］. Plant Physiology, 1998, 118(3): 725-732

［6］韩锁义，杨玛丽，陈远东，等. 大豆“南农94-16”突变体库的构建及部分性状分析［J］.核农学报，2008, 22(2): 131-135. (Han S Y, Yang M L, Chen Y D, et al. Construction of mutant library for soybean ‘Nannong 94-16’ and analysis of some characters［J］. Journal of Nuclear Agricultural Sciences, 2008, 22(2): 131-135.)

［7］韩锁义，张恒友，杨玛丽，等. 大豆“南农86-4”突变体筛选及突变体库的构建［J］. 作物学报，2007, 33(12): 2059- 2062. (Han S Y, Zhang H Y, Yang M L, et al. Screening of mutants and construction of mutant population in soybean ‘Nannong 86-4’［J］. Acta Agronomica Sinica, 2007, 33(12): 2059- 2062.)

［8］陈远东，喻德跃. EMS诱发大豆“南农94-16”突变体库的扩建及部分突变体的SSR分析［J］大豆科学，2009, 28(4): 574-577. (Chen Y D, Yu D Y. Construction of mutant pools for soybean ‘Nannong 94-16’ induced by EMS and analysis of SSR marker on several mutants［J］.Soybean Science, 2009, 28(4): 574-577.)

［9］周蓉，王贤智，陈海峰，等. 大豆倒伏性及其相关性状的QTL分析［J］. 作物学报, 2009, 35(1): 57-65. (Zhou R, Wang X Z, Chen H F, et al. QTL analysis of lodging and related traits in soybean［J］. Acta Agroniomica Sinica, 2009, 35(1): 57-65.)

［10］Pedziwilk F.Ethyl methanesulfonate (EMS)induced mutants of Propionibacterium shermanii 2 ［J］.Acta microbiologica Polonica- Series B: Microbiologia Applicata, 1971, 3(2): 79

［11］张俐俐，雷勃钧，吕晓波，等. 应用化学诱变法筛选抗草甘膦大豆突变株系［J］. 大豆科学, 2009, 28(5): 938-940. (Zhang L L, Lei B J, Lyu X B, et al. Glyphosate resistant mutant strain of soybean filtered be chemomorphsis［J］. Soybean Science, 2009, 28(5): 938-940.)

［12］张力伟，牛腾飞，张文会，等. 大豆“冀黄13”突变体筛选及突变体库的建立［J］大豆科学, 2013, 32(1): 33-37. (Zhang L W, Niu T F, Zhang W H, et al. Screening of mutants and construction of mutant population for soybean cultivar “Jihuang13”［J］. Soybean Science, 2013, 32(1): 33-37.)

［13］Chiu W H, Chandler J, Cnops G, et al. Mutations in the TORNAD02 gene affect cellular decision in the peripheral zone of the shoot apical meristem of Arobidopsis thaliana［J］ Plant Molecular Biology, 2007, 63(6): 731-744.

［14］Koornneeff M, Dellaert L,van der Veen J H, et al. EMS and relation-induced mutation frequencies at individual loci in Arabidopsis thaliana(L) Heynh［J］.Mutation Research, 1982, 93(1): 109-123.

［15］曲高平, 孙妍妍, 庞红喜, 等. 甘蓝型油菜EMS突变体库构建及抗除草剂突变体筛选［J］. 中国油料作物学报, 2014, 36(1): 25-31. (Qu G P, Sun Y Y, Pang H X, et al. EMS mutagenesis and ALS.inhibitor herbicide-resistant mutants of Brassica napus L ［J］ Chinese Journal of Oil Crop Sciences, 2014, 36(1): 35-31.)

［16］Kuraparthy V, Sood S, Dhaliwal H S, et al. Identification and mapping of a tiller inhibition gene(tin-3)in wheat［J］. Theoretical and Applied of Genetics, 2007, 114(2): 285-294.

［17］徐艳花, 陈锋, 董中东, 等. EMS诱变的普通小麦豫农201突变体库的构建与初步分析［J］. 麦类作物学报, 2010, 30(4): 625-629.(Xu Y H, Chen F, Dong Z D, et al. Construction and analysis of EMS induced mutant library of hexaploid wheat cultivar Yunong 201［J］. Journal of Triticeae Crops, 2010, 30(4): 625-629.)

［18］Krishnan A, Guiderdoni E, An G, et al.Mutant resources in rice for the functional genomics of the grasses ［J］. Plant Physiology, 2009, 149: 165-170.

［19］叶俊, 吴建国, 郑希, 等.水稻“9311”突变体筛选和突变体库构建［J］. 作物学报, 2006, 32(10): 1525-1529. (Ye J, Wu J G, Zheng X, et al. The screening of mutants and construction of mutant population for cultivar “9311” in rice (Oryza sativa L)［J］. Acta Agronomica Sinica, 2006, 32(10): 1525-1529.)

［20］Zhao Y, Wang M L, Zhang Y Z, et al. Achlorophyll-reduced seedling mutant in oilseed rape, Brassica napus for utilization in F₁?hybrid production ［J］. Plant Breeding, 2000,119(2): 131-135.

［21］Tadege M, Wang T L, Wen J Q, et al. Mutagenesis and beyond! Tools for understanding legume biology ［J］ Plant Physiology, 2009,151(3): 978-984.

［22］张达，王军虹，王豫颖，等. 矮化大豆突变体GA₃的调控［J］.大豆科学, 2008, 27(3): 456-460. (Zhang D, Wang H J, Wang Y Y, et al. GA₃?regulation in the dwarfing mutant soybean［J］. Soybean Science, 2008, 27(3): 456-460.)

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备注/Memo

基金项目：国家重点基础研究发展计划“973计划”（2011CB100402）；国家现代农业产业技术体系（CARS-04-PS19）。第一作者简介：孟永杰（1990-），男，硕士，主要从事作物高产优质栽培理论与技术研究。通讯作者：舒凯（1982-），男，博士，副研究员，主要从事大豆遗传学和生理学研究。E-mail: kshu@sicau.edu.cn；杨文钰（1958-），男，教授，博导，主要从事作物栽培生理生态与区域农业研究。E-mail: mssiyangwy@sicau.edu.cn。

更新日期/Last Update: 2016-01-28