[1]王久镇,王丹华,马占洲,等.野生大豆染色体片段代换系百粒重的选择牵连效应及响应分析[J].大豆科学,2015,34(06):950-956.[doi:10.11861/j.issn.1000-9841.2015.06.0950]
　WANG Jiu-zhen,WANG Dan-hua,MA Zhan-zhou,et al.Hitchhiking Effect and Responses Analysis about 100-Seed Weight of Wild Soybean Chromosome Segment Substitution Lines[J].Soybean Science,2015,34(06):950-956.[doi:10.11861/j.issn.1000-9841.2015.06.0950]
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野生大豆染色体片段代换系百粒重的选择牵连效应及响应分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年06期 页码: 950-956 栏目: 出版日期: 2015-12-25
Title:
Hitchhiking Effect and Responses Analysis about 100-Seed Weight of Wild Soybean Chromosome Segment Substitution Lines
作者:
王久镇1; 王丹华1; 马占洲1; 谷月1; 曾庆力1; 刘春燕2; 蒋洪蔚2; 陈庆山1
1.东北农业大学 农学院，黑龙江 哈尔滨 150030；
2.黑龙江省农垦科研育种中心，黑龙江 哈尔滨 150090
Author(s):
WANG Jiu-zhen1;  WANG Dan-hua1;  MA Zhan-zhou1; GU Yue1; ZENG Qing-li1;  LIU Chun-yan2;  JIANG Hong-wei2;  CHEN Qing-shan1
1. Agronomy College, Northeast Agricultural University, Harbin 150030, China;?
2.The Crop Research and Breeding Center of Land-Reclamation of Heilongjiang Province, Harbin 150090, China
关键词:
大豆; 染色体片段代换系; 选择牵连效应; 响应分析
Keywords:
Soybean;  Chromosome segment substitution lines;  Hitchhiking effect;  Responses analysis
DOI:
10.11861/j.issn.1000-9841.2015.06.0950
文献标志码:
A
摘要:
利用野生大豆导入系群体和多位点扫描分析方法研究了针对百粒重性状的选择牵连效应，找到与百粒重性状相关联的位点，并分析了这些位点在选择条件的响应。随机群体28.10%的位点出现偏分离（P＜0.05），经百粒重选择后等位基因偏分离比例减少，偏分离程度加大。标记异常偏分离率缩小到4.13%和23.14%，卡方值变幅缩小至0~166.67和0.01~81.30。对随机群体和选择群体的供体片段导入频率，在P＜0.05水平下进行卡方分析，定位到与百粒重相关的的12个位点。
Abstract:
The hitchhiking effects in the advanced wild soybean chromosome segment substitution lines with selection under 100-seed weight conditions were analyzed by scanning multiple molecular loci.The lociare connected with 100-seed weight and their response in selection condition were analysized.28.10% of the loci in random population were detected with segregation distortion（P＜0.05）, after selection in view of 100-seed weight, the segregation distortion of allelic genes was reduced but the degree of segregation distortion increased.The segregation distortion ratio reduced to 4.13% and 23.14%, the variation of chi-square reduced to 0-166.67 and 0.01-81.30. Twelve putative 100-seed weight related loci were obtained using chi-square test (P＜0.05) by comparison of the introgressive frequency of alleles between random population and selected population.

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备注/Memo

基金项目：教育部新世纪优秀人才支持计划(NECT-1207-01)；黑龙江省自然科学基金重点项目（ZD201213）；国家现代农业产业技术体系（CARS-04-02A）；黑龙江省博士后基金（LBH-Z12035）；中国博士后基金（2012M520030）；黑龙江省高校长江后备支持计划项目（2014CJHB004）。

第一作者简介：王久镇（1989-），男，硕士，主要从事作物遗传育种研究。E-mail：wjzwjz11589@163.com。
通讯作者：蒋洪蔚（1983-），男，博士,高级农艺师，主要从事大豆生物技术研究。E-mail：j3994102@126.com。陈庆山（1973-），男，教授，博导，主要从事大豆生物技术研究。E-mail：qshchen@126.com。

更新日期/Last Update: 2015-12-31