[1]胡振帮,刘航,辛大伟,等.基于野生大豆导入系的大豆粒长和粒宽的QTL分析[J].大豆科学,2015,34(06):945-949.[doi:10.11861/j.issn.1000-9841.2015.06.0945]
　HU Zhen-bang,LIU Hang,XIN Da-wei,et al.QTL Analysis of Seed Length and Seed Width by the Wild Soybean Genome Introgression Lines[J].Soybean Science,2015,34(06):945-949.[doi:10.11861/j.issn.1000-9841.2015.06.0945]
点击复制

基于野生大豆导入系的大豆粒长和粒宽的QTL分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年06期 页码: 945-949 栏目: 出版日期: 2015-12-25
Title:
QTL Analysis of Seed Length and Seed Width by the Wild Soybean Genome Introgression Lines
作者:
胡振帮1; 刘航1; 辛大伟1; 蒋洪蔚1; 2; 朱荣胜1; 陈庆山1; 齐照明1; 刘春燕2
1.东北农业大学 大豆研究所/大豆生物学教育部重点实验室，黑龙江 哈尔滨 150030；
2.黑龙江省农垦科研育种中心，黑龙江 哈尔滨 150030
Author(s):
HU Zhen-bang 1;  LIU Hang1;  XIN Da-wei1;  JIANG Hong-wei1; 2; ZHU Rong-sheng1;  CHEN Qing-shan1;  QI Zhao-ming1;  LIU Chun-yan2
1.Soybean Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry /Key Laboratory of Biology and Genetics & Breeding for Soybean in Northeast China, Ministry of Agriculture, Harbin 150030, China；
2. The Crop Research and Breeding Center of Land-Reclamation of Heilongjiang Province, Harbin 150030，China
关键词:
大豆; 粒长; 粒宽; QTL
Keywords:
Soybean;  Seed length;  Seed width;  QTL
DOI:
10.11861/j.issn.1000-9841.2015.06.0945
文献标志码:
A
摘要:
以野生大豆ZYD00006（父本）和绥农14 （母本）为亲本构建的导入系为定位群体，群体含有102个株系，遗传图谱使用了329个SSR标记位点，针对粒长和粒宽2个籽粒性状进行定位分析。结果表明：有18个位点存在不同性状间被同时检测到的情况，其中与QTL连锁的位点Sat_227、Satt338和Satt568被2个性状同时检测到。通过比较分析也发现3个位点也同时影响着百粒重的大小，进一步通过加性效应分析表明，定位区段对粒长的影响为-10%~15.4%，定位区段对粒宽的影响为-26.72%~2.76%。明确导入位点对粒长和粒宽的影响，可以作为进一步大豆粒长和粒宽相关基因挖掘。
Abstract:
Using the chromosome fragment introgression lines of soybean derived from the cross between ZYD00006 (Glycine soja) as male parent and Suinong 14 (Glycine max) as female parent， to study location of seed length and seed width.This population has 102 lines and 329 SSR markers were used to construct the genetic map. In the results, 18 different traits were found underlying those two seed traits. And the QTL sites of Sat_227, Satt388 and Satt568 were found underlying those two traits at the same time.By the comparison, these 3 sites were found in different genetic population for seed traits.The additive effect of location interval underlying seed length was range from -10% to 15.4%, and the additive effect of location interval underlying seed width was range from -26.72% to 2.76%. So these markers was useful to molecular assistant breeding and gene mining。

参考文献/References:

［1］Salas P, Oyarzo-Llaipen J C, Wang D, et al. Genetic mapping of seed shape in three populations of recombinant inbred lines of soybean (Glycine max L Merr)［J］.Theoretical and Applied Genetics, 2006, 113(8): 1459-1466.

［2］Moongkanna J, Nakasathien S, Novitzky W P, et al.SSR markers linking to seed traits and total oil content in soybean［J］. The Journal of Agricultural Science, 2011,44(4): 233-241.
［3］Xu Y, Li H N, Li G J, et al. Mapping quantitative trait loci for seed size traits in soybean (Glycine max L Merr)［J］. Theoretical and Applied Genetics, 2011, 122(3): 581-594.
［4］Niu Y, Xu Y, Liu X F, et al. Association mapping for seed size and shape traits in soybean cultivars［J］. Molecular Breeding, 2013, 31(4): 785-794
［5］邱丽娟，常汝镇.大豆种质资源描述规范和数据标准［M］.北京:中国农业出版社，2006.（Qiu L J, Chang R Z. Rule and standardion of soybean germplasm［M］. Beijing: China Agricultural Press, 2006.）
［6］Doyle J J. Isolation of plant DNA from fresh tissue ［J］. Focus, 1990, 12: 13-15.
［7］McCough S R, Doerge R W. QTL mapping in rice ［J］. Trends in Genetics, 1995, 11(12): 482-487.
［8］Eshed Y, Zamir D. An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL［J］. Genetics, 1995,141: 1147-1162.
［9］Li M, Sun P, Zhou H, et al. Identification of quantitative trait loci associated with germination using chromosome segment substitution lines of rice (Oryza sativa L.)［J］. Theoretical and Applied Genetics, 2011, 123(3): 411-420.
［10］Koide Y, Fujita D, Tagle A G, et al. QTL for spikelet number from a high-yielding rice variety, Hoshiaoba, detected in an introgression line with the genetic background of an indica rice variety, IR64［J］. Euphytica, 2013, 192(1): 97-106.
［11］Qi H, Huang J, Zheng Q, et al. Identification of combining ability loci for five yield-related traits in maize using a set of testcrosses with introgression lines［J］. Theoretical and Applied Genetics, 2013, 126(2): 369-377.
［12］Davoyan E R, Davoyan R O, Bebyakina I V, et al. Identification of a leaf-rust resistance gene in species of Aegilops L., synthetic forms, and introgression lines of common wheat ［J］. Russian Journal of Genetics: Applied Research, 2012, 2(4): 325-329.
［13］Lei M P, Li G R, Zhou L, et al. Identification of wheat-secale africanum chromosome 2Rafr introgression lines with novel disease resistance and agronomic characteristics ［J］. Euphytica, 2013, 194(2): 197-205.
［14］Zhang W B, Qiu P C, Jiang H W, et al. Dissection of genetic overlap of drought and low-temperature tolerance QTLs at the germination stage using backcross introgression lines in soybean［J］. Molecular Biology Reports, 2012, 39(5): 6087-6094.
［15］Wang W, He Q, Yang H, et al. Development of a chromosome segment substitution line population with wild soybean (Glycine soja Sieb. et Zucc.) as donor parent［J］. Euphytica, 2013, 189(2): 293-307.
［16］Liu W X, Kim M Y, van K Y, et al. QTL identification of yield-related traits and their association with flowering and maturity in soybean［J］. Journal of Crop Science and Biotechnology, 2011, 14(1): 65-70.
［17］Liang H, Yu Y L, Yang H Q, et al. Inheritance and QTL mapping of related root traits in soybean at the seedling stage［J］. Theoretical and Applied Genetics, 2014, 127(10): 2127-2137.
［18］Smalley M D, Fehr W R, Cianzio S R, et al. Quantitative trait loci for soybean seed yield in elite and plant introduction germplasm［J］. Crop Science, 2004, 44: 436-442.
［19］Zhang Y H, He J B, Wang Y F, et al. Establishment of a 100-seed weight quantitative trait locus-allele matrix of the germplasm population for optimal recombination design in soybean breeding programmes［J］. Journal of Experimental Botany, 2015, doi: 10.1093/jxb/erv342.
［20］Du W J, Yu D Y, Fu S X. Detection of quantitative trait loci for yield and drought tolerance traits in soybean using a recombinant inbred line［J］. Population Journal of Integrative Plant Biology, 2009, 51 (9): 868-878.
［21］Hao D, Cheng H, Yin Z, et al. Identification of single nucleotide polymorphisms and haplotypes associated with yield and yield components in soybean (Glycine max) landraces across multiple environments［J］. Theoretical and Applied Genetics. 2012; 124:447-458.
［22］Foxac M C, Carya R T, Nelson L R, et al. Confirmation of a seed yield QTL in soybean［J］. Crop Science, 2014, 55(3): 992-998.
［23］Kato S, Fujii K, Yumoto S, et al. Seed yield and its components of indeterminate and determinate lines in recombinant inbred lines of soybean［J］. Breeding Science, 2015, 65(2): 154-160.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(06):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(06):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(06):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(06):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(06):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(06):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(06):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(06):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(06):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(06):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

基金项目：教育部新世纪优秀人才支持计划(NECT-1207-01)；国家自然基金面上项目（31271747，31471516）；国家自然科学基金青年基金(31400074，31401465)；黑龙江省自然科学基金重点项目(ZD201213)；黑龙江省博士后基金(LBH-Z12035)； 中国博士后基金(2012M520030)； 黑龙江省高校长江后备支持计划项目(2014CJHB004)。

第一作者简介：胡振帮（1978 -）,男，硕士，实验师,主要从事大豆表型网络及导入系构建研究。E-mail:hzb_net@126.com。
通讯作者：刘春燕（1981-），女，博士，副研究员，主要从事大豆生物技术研究。E-mail：cyliucn@126.com。

更新日期/Last Update: 2015-12-31