CHEN Qing-shan,JIANG Hong-wei,SUN Dian-jun,et al.QTL Mapping for 100 seed Weight Using Wild Soybean Chromosome Segment Substitution Lines[J].Soybean Science,2014,33(02):154-160.[doi:10.11861/j.issn.1000-9841.2014.02.0154]
利用野生大豆染色体片段代换系定位百粒重QTL
- Title:
- QTL Mapping for 100 seed Weight Using Wild Soybean Chromosome Segment Substitution Lines
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 利用野生大豆ZYD00006(供体亲本)与黑龙江省主栽品种绥农14(轮回亲本)所构建的高世代(BC3)染色体片段代换系130个株行进行QTL定位。采用基于单标记的方差分析方法检测到25个百粒重相关的SSR位点，为避免由于标记位点共分离而产生的假阳性结果，对方差分析得到的相邻位点进行代换作图分析，最终获得分布于大豆10条连锁群上的19个百粒重相关位点。其中有7个位点与已有研究结果完全一致；2个位点与已有研究结果位置相距0.9和4.6 cM；其余10个位点首次发现，推测是本套材料的特有位点；其中位点QSW-D1a-2加性效应3.6，QSW-H-2加性效应-2.1，片段长度均小于10 cM，可作为继续研究的首选位点。
- Abstract:
- A chromosome segment substitution lines(BC3)including 130 lines was constructed by the cross of wild soybean ZYD00006(donor parent)and cultivar Suinong 14(recurrent parent).The QTL underlying 100 seed weight was identified using ANOVA Method based on single marker with trait.Twenty five SSR markers underlying 100 seed weight were detected with ANOVA method.To avoid the false positive of co segregation markers,substitution mapping was used to verify the result of ANOVA method.Finally,Nineteen QTL underlying 100 seed weight were identified using two methods and those QTL distributed on 10 linkage groups.Seven QTL were in full accord with known results;two QTL were somewhat different with known results of 0.9 cM or ?4.6 cM ?distance.Another 10 ones were first discovery of loci,which should be specific loci in the study.QSW-D1a2 and QSWH2 with 3.6 and -2.1 of additive effects,fragments length were less than 10 cM could be used as the first choice loci for further study.In this study,substitution lines which had similar genetic background were used to QTL mapping.The result of QTL mapping is more credible because there is no interference of genetic background.Specific materials and important loci lay a foundation for further study on 100seed weight QTL fine mapping and molecular assisted breeding.
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备注/Memo
基金项目:教育部新世纪优秀人才支持计划(NECT-1207-01)；黑龙江省自然科学基金重点项目(ZD201213)；现代农业产业体系(CARS-04-02A)；黑龙江省博士后基金(LBH-Z12035);中国博士后基金(2012M520030)；黑龙江省高校长江后备支持计划项目(2014CJHB004)。
第一作者简介：陈庆山(1973-)，男，博士，教授，博士生导师，主要从事大豆生物技术研究。E-mail：qshchen@126.com。
通讯作者：胡国华(1951-)，男，博士，研究员，主要从事大豆遗传育种与栽培研究。E-mail：hugh757@vip.163.com。