GUO Qiu-yun,WANG Ping,LIU Zhao-pu.Effect of IBA and 6-BA Ratio on Adventitious Shoot Induction from Hypocotyl in Soybean[J].Soybean Science,2012,31(05):725-730.[doi:10.3969/j.issn.1000-9841.2012.05.007]
IBA和6-BA浓度配比对诱导大豆下胚轴不定芽的影响
- Title:
- Effect of IBA and 6-BA Ratio on Adventitious Shoot Induction from Hypocotyl in Soybean
- 文章编号:
- 1000-9841（2012）05-0725-06
- Keywords:
- Soybean; Hypocotyl; Adventitious shoot; IBA; 6-BA
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以大豆黑农44下胚轴为外植体，研究不定芽诱导培养基中IBA和6-BA浓度及配比对不定芽诱导与伸长的影响。结果表明，以MS基本培养基+1.0 mg·L-16-BA为大豆无菌苗培养基，在诱导培养基中不添加6-BA时有利于下胚轴不定芽的诱导，添加0.1 mg·L-1IBA时，培养14 d不定芽诱导率可达96.69%，每个下胚轴形成2.31个芽（芽长≥0.5 cm）；不定芽诱导培养基中添加高浓度6-BA和IBA组合不利于大豆下胚轴不定芽的诱导与伸长。
- Abstract:
- Hypocotyl from soybean Heinong 44 was used as the explants to investigate the effects of IBA and 6-BA and the concentration ratio on the adventitious shoot induction and elongation.The higher induction rate of adventitious shoots from hypocotyls was observed in the induction MS medium without 6-BA while the seeds germinated on MS medium+1.0 mg·L-16-BA.Induction rate and number of adventitious shoots reached 96.69% and 2.31(shoot length≥0.5 cm)respectively cultured for 14 days on induction MS medium with addition of 0.1 mg?L-1IBA.Shoot induction medium with higher concentration of 6-BA and IBA significantly reduced the induction and elongation of adventitious shoots from hypocotyl of soybean.
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备注/Memo
基金项目：国家转基因生物新品种培育重大专项（2009ZX08010-013B）; 中央财政支持地方高校发展专项资金资助（CXTD07）。