[1]于洪久,朱洪德,刘丽君,等.腐霉菌侵染条件下大豆下胚轴中PAL和POD活性的变化[J].大豆科学,2012,31(03):495-497.[doi:10.3969/j.issn.1000-9841.2012.03.034]
　YU Hong-jiu,ZHU Hong-de,LIU Li-jun,et al.PAL and POD Activities of Soybean Hypocotyls Infected by Pythium [J].Soybean Science,2012,31(03):495-497.[doi:10.3969/j.issn.1000-9841.2012.03.034]
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腐霉菌侵染条件下大豆下胚轴中PAL和POD活性的变化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年03期 页码: 495-497 栏目: 出版日期: 2012-03-25
Title:
PAL and POD Activities of Soybean Hypocotyls Infected by Pythium
文章编号:
1000-9841（2012）03-0495-03
作者:
于洪久1; 2; 朱洪德1; 刘丽君3; 唐晓飞3; 魏 崃3
1.黑龙江八一农垦大学 农学院，黑龙江 大庆163319；
2.黑龙江省农业科学院 农村能源研究所，黑龙江 哈尔滨150086；
3.黑龙江省农业科学院 大豆研究所，黑龙江 哈尔滨150086
Author(s):
YU Hong-jiu1; 2; ZHU Hong-de1; LIU Li-jun3; TANG Xiao-fei3; WEI Lai3
1.Agronomy College,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang;
2.Rural Energy Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang;
3.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China
关键词:
腐霉菌; 大豆; 下胚轴; 苯丙氨酸解氨酶（PAL）; 过氧化物酶（POD）
Keywords:
Pythium aphanidematum; Soybean; Hypocotyls; Phenylalanine ammonia-lyase(PAL); Peroxidase(POD)
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.03.034
文献标志码:
A
摘要:
以对腐霉菌抗性不同的6个大豆品种为材料，测定接种腐霉菌（Pythium aphanidematum）后下胚轴的中的PAL和POD活性，分析大豆与腐霉菌互作过程中，不同抗性品种下胚轴中PAL和POD的活性变化规律。结果表明：接种腐霉菌后，抗病品种下胚轴的PAL活性呈先升高后降低，再次升高后降低的变化趋势；感病品种的PAL活性呈先升高后降低的趋势；不同抗感性品种接种腐霉菌后下胚轴的POD活性均不断升高；抗病品种POD活性增加速度、PAL和POD活性峰值均高于感病品种。
Abstract:
The PAL and POD activities of soybean hypocotyls are closely correlated with soybean disease resistance.In this study,six soybean varieties with different resistance to Pythium were inoculated with Pythium aphanidematum and determined the PAL and POD activities of hypocotyls.The result showed that with the extension of inoculating time,PAL activities of resistant cultivar increased firstly,and then decreased,after that increased and finally decreased;PAL activities of susceptible varieties increased firstly and then decreased.The POD activities of resistant and susceptible soybeans increased gradually,but the increasing speed and the maximum of PAL and POD activities of resistant cultivars were higher than those of susceptible cultivars.

参考文献/References:

[1] 王敬文 , 薛应龙 . 植物苯丙氨酸解氨酶的研究Ⅰ [J]. 植物生理学报 ,1981,7(4):373-375. （ Wang J W,Xue Y L.Research on plant phenylalanine ammonia-lyase Ⅰ [J].Plant Physiology Journal,1981,7(4):373-375. ）

[2]刘亚光,李海英,杨庆凯.大豆品种的抗病性与叶片内苯丙氨酸解氨酶活性关系的研究[J].大豆科学,2002,21(3):195-198.(Liu Y G,Li H Y,Yang Q K.Study on the relationship between resistance of soybean and activity of PAL in leaves of soybean infected by Cercospora sojinaHara[J].Soybean Science,2002,21(3):195-198.)

[3]孙欣,刘丽君,薛永国,等.接种疫霉根腐菌对大豆苯丙氨酸解氨酶活性的影响[J].大豆科学,2008,27(4):641-644.(Sun X,Li L J,Xue Y G,et al.Changes of L-phenylalanin ammo-nialyase activity in soybean inoculated with Phytophthora sojae[J].Soybean Science,2008,27(4):641-644.)

[4]梁艳荣,胡晓红,张颖力,等.植物过氧化物酶生理功能研究进展[J].内蒙古农业大学学报,2003,24(2):110-113.)(Liang Y R,Hu X H,Zhang Y L,et al.Progress on physiological function research of plant peroxidase[J].Journal of Inner Mongolia Agricultural University,2003,24(2):110-113.)

[5]蒋选利,李振岐,康振生.过氧化物酶与植物抗病性研究进展[J].西北农林科技大学学报(自然科学版),2001,29(6):124-129.(Jiang X L,Li Z Q,Kang Z S.The recent progress of research on peroxidase in plant disease resistance[J].Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition),2001,29(6):124-129.)

[6]吴俊江,刘丽君,高明杰,等.大豆接种疫霉根腐病菌后过氧化物酶活性的变化[J].中国油料作物学报,2003,25(3):67-70.(Wu J J,Liu L J,Gao M J,et al.The change of POD activity in soybean varieties after inoculation with Phytophthora sojae[J].Chinese Journal of Oil Crop Sciences,2003,25(3):67-70.)

[7]Hartman G L,Sinclair J B,Rupe J C.Compendium of soybean diseases[M].St Paul:American Phytopathological Society Press,1999:42-44.

[8]郝再彬,苍晶,徐伸.植物生理学实验[M].北京:中国农业出版社,2004.(Hao Z B,Chang J,Xu S.Plant Physiology Experiment[M].Beijing:China Agriculture Press,2004.

[9]郑殿峰,梁喜龙,左豫虎,等.大豆根腐病菌对大豆幼苗生理生化指标的影响[J].中国油料作物学报,2004,26(3):57-61.(Zheng D F,Liang X L,Zuo Y H,et al.Pathogen(Fusarium oxysporum)of soybean root rot impacting on biochemical and physiological indexes of soybean seedling[J].Chinese Journal of Oil Crop Sciences,2004,26(3):57-61.)

[10]金庆超,叶华智,张敏.苯丙氨酸解氨酶活性与玉米对纹枯病抗性的关系[J].四川农业大学学报,2003,21(2):116-118.(Jin Q C,Ye H Z,Zhang M.Relationship between the activity of PAL and resistance of corn to maize sheath blight[J].Journal of Sichuan Agricultural University,2003,21(2):116-118.)

[11]周博如,刘太国,杨微,等.不同抗性的大豆品种感染细菌性疫病后POD、PPO变化的研究[J].大豆科学,2002,21(3):183-186.(Zhou B R,Liu T G,Yang W,et al.Study on the change of POD activity and PPO activity in soybean varieties with different resistance toPseudomonas syringaepv.Glycinea(PSG)[J].Soybean Science,2002,21(3):183-186.)

[12]曾水三,王振中.豇豆与锈菌互作中的多酚氧化酶和过氧化物酶活性及其与抗病性的关系[J].植物保护学报,2004,31(2):145-150.(Zeng S S,Wang Z Z.Relationships between activities of polyphenol oxidase and peroxidase,and resistance of cowpea to Uromyces vignae[J].Acta Phylacica Sinica,2004,31(2):145-150.)

[13]邢会琴,李敏权,徐秉良,等.过氧化物酶和苯丙氨酸解氨酶与苜蓿白粉病抗性的关系[J].草地学报,2007,15(4):376-380.(Xing H Q,Li M Q,Xu B L,et al.Relationships between leaf POD and PAL and the resistance of Alfalfacultivars against powdery mildew[J].Acta Agrestia Sinica,2007,15(4):376-380.)

[14]王安乐,邓稳桥,朱海全,等.黄瓜感染霜霉病菌后关键酶活性的变化[J].长江蔬菜,2008（10）:52-54.(Wang A L,Deng W Q,Zhu H Q，et al.On the change of enzyme activites of cucumber infected by Pseudoperonospora[J].Journal of Changjiang Vegetables,2008(10):52-54.)

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备注/Memo

基金项目：国家科技支撑计划资助项目（2011BAD35B06）；国家农业科技成果转化项目（2010GB2B200128）；黑龙江省科技厅攻关计划资助项目（GA09B101-1-12）。

第一作者简介：于洪久（1981-），男，助理研究员，在读硕士，研究方向为大豆遗传育种。E-mail:yhj3130618@126.com。
通讯作者：朱洪德（1962-），男，研究员，博士生导师，主要从事大豆遗传育种研究。E-mail:zhd495@163.com。刘丽君（1958-），女，研究员，主要从事大豆遗传育种研究。E-mail:nkyssbd@126.com。

更新日期/Last Update: 2014-08-17