WU Shuai,WANG Zhi-kun,LAN Lan,et al.Screening of the Optimal Acceptor Genotypes in Introduced Soybean Germplasm[J].Soybean Science,2012,31(01):29-33.[doi:10.3969/j.issn.1000-9841.2012.01.007]
引进大豆种质遗传转化适用基因型的筛选
- Title:
- Screening of the Optimal Acceptor Genotypes in Introduced Soybean Germplasm
- 文章编号:
- 1000-9841（2012）01-0029-05
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以引进种质“越黑”、“越褐”、Moshidou Gong503（半野生种）、日A、日B1、日B2的子叶节为受体材料,采用农杆菌介导法转入抗虫基因cryI，筛选组织培养适应性强，转化效率高的引进大豆种质。并对适宜遗传转化的基因型在萌发阶段和芽诱导阶段的适宜6-BA浓度进行筛选。结果表明：参试品种中“越褐”为适用于子叶节器官发生途径的基因型；萌发阶段添加浓度为0.1 mg·L-1的6-BA，可获得最佳轴根比，此时的无菌苗下胚轴粗壮无须根；萌发阶段和芽诱导阶段6-BA的最佳浓度分别为0.1和1.7 mg·L-1。
- Abstract:
- cryI gene was transformed into soybean cotyledon nodes of 6 foreign soybean germplasm by Agrobacterium-midiated method and screened the optimal acceptor genotype by comparing the callus induction,regeneration and etiolate rate.Then the suitable 6-BA concentration for germination and induction mediums of the optimal genotype was studied.The results indicated that the induction,differentiation and etiolation rate were obviously different between soybean genotypes.Among the 6 introduced soybean germplasm,‘Yuehe’ was optimal for tissue culture and genetic transformation.The best hypocotyl-radicel ratio could be obtained when the concentration of 6-BA was 0.1 mg·L-1.The optimal concentration of 6-BA for ‘Yuehe’ transformation was 0.1 and 1.7 mg·L-1in germination medium and bud induction medium,respectively.
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备注/Memo
基金项目：国家自然科学基金资助项目（31071440）；黑龙江省普通高等学校青年骨干支持计划项目（1155G12）；博士后研究人员落户黑龙江科研启动项目(2009HB009)；转基因生物新品种培育重大专项资助项目（2011ZX08004-004-006-002）。