[1]何道一,张艳梅,张坤亮.大豆幼胚培养直接成苗影响因素研究[J].大豆科学,2012,31(01):34-37.[doi:10.3969/j.issn.1000-9841.2012.01.008]
　HE Dao-yi,ZHANG Yan-mei,ZHANG Kun-liang.Influence Factors of Plantlet Regeneration Directly from Cultured Young Embryos in Soybean[J].Soybean Science,2012,31(01):34-37.[doi:10.3969/j.issn.1000-9841.2012.01.008]
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大豆幼胚培养直接成苗影响因素研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年01期 页码: 34-37 栏目: 出版日期: 2012-02-25
Title:
Influence Factors of Plantlet Regeneration Directly from Cultured Young Embryos in Soybean
文章编号:
1000-9841（2012）01-0034-04
作者:
何道一; 张艳梅; 张坤亮
淮北师范大学 生命科学学院 资源植物生物学安徽省重点实验室，安徽 淮北 235000
Author(s):
HE Dao-yi; ZHANG Yan-mei; ZHANG Kun-liang
Anhui Key Laboratory of Resources and Plant Biology，School of Life Sciences，Huaibei Normal University，Huaibei 235000，Anhui，China
关键词:
大豆; 幼胚培养; 成苗
Keywords:
Soybean; Young embryo culture; Plantlet
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.01.008
文献标志码:
A
摘要:
为建立大豆幼胚培养直接成苗方法，分别研究了大豆幼胚胚龄、幼胚预处理方法、培养基及大豆品种对大豆幼胚培养直接成苗的影响。结果表明：15 d胚龄的幼胚平均萌发率为23%，20 d及以上胚龄的幼胚萌发率高于70%，20 d胚龄的幼胚虽能萌发，但芽和根的正常生长较为困难。在低温（4℃）、高温（37℃）和GA(100 mmol·L-1)预处理幼胚2 d再进行培养条件下，高温预处理平均成苗率达75%；而低温和GA预处理的成苗率只有4%。所试几种培养基对幼胚成苗效果没有显著影响。不同品种中，极早熟品种日本晴和台农292成苗率分别为74%和72%，早熟品种台湾75和苏早1号成苗率均为64%，夏大豆临豆8号成苗率（54%）最低。
Abstract:
In order to establish the method of plantlet regeneration directly from cultured young embryo of soybean，some influencing factors including embryo age，pre-treatment methods，cultivation medium and soybean genotypes，were investigated respectively.Germination rate of 15 d young embryo was 23%，and that of 20 d or elder young embryo was more than 70%.Although 20 d young embryo could germinate，the development of its bud and root was difficult.Among the 2 days pre-treatment of low temperature（4℃），high temperature（37℃） and GA buffer(100 mmol·L-1)，high-temperature promoted the plantlet rate(75%)of young embryo，while no significant effect were observed for other treatmeats.The tested cultivation medium containing different external hormones had no obvious effect on plant regeneration.The plantlet rate of soybean genotypes varied with maturity groups，and that of extremely early-mature soybean were highest，with Ribenqing and Tainong 292 were 74% and 72%，respectively.

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备注/Memo

基金项目：资源植物生物学安徽省重点实验室开放课题（KLPRB200910）。

第一作者简介：何道一（1963-），男，博士，教授，主要从事植物分子生物学研究。E-mail:daoyi1h@sohu.com。

更新日期/Last Update: 2014-08-15