[1]张书利,刘丽君,唐晓飞,等.利用农杆菌介导法将BrCS基因导入大豆的研究[J].大豆科学,2011,30(04):569-573.[doi:10.11861/j.issn.1000-9841.2011.04.0569]
　ZHANG Shu-li,LIU Li-jun,TANG Xiao-fei,et al.Transformation of BrCS Gene into Soybean by Agrobacterium-mediated Method[J].Soybean Science,2011,30(04):569-573.[doi:10.11861/j.issn.1000-9841.2011.04.0569]
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利用农杆菌介导法将BrCS基因导入大豆的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第30卷 期数: 2011年04期 页码: 569-573 栏目: 出版日期: 2011-08-25
Title:
Transformation of BrCS Gene into Soybean by Agrobacterium-mediated Method
文章编号:
1000-9841（2011）04-0569-05
作者:
张书利1; 2; 刘丽君1; 唐晓飞1; 魏崃2
1.黑龙江省农业科学院 大豆研究所，黑龙江 哈尔滨 150086;
?2.东北农业大学 农学院,黑龙江 哈尔滨 150030
Author(s):
ZHANG Shu-li1; 2; LIU Li-jun1; TANG Xiao-fei1; WEI Lai2
1.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086;
?2.College of Agriculture，Northeast Agricultural University,Harbin 150030 Heilongjiang, China
关键词:
大豆; 遗传转化; BrCS基因; 抗冷性; 耐盐性
Keywords:
Soybean; Genetic transformation; BrCS gene; Cold-stress tolerance; Salt-stress tolerance
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.04.0569
文献标志码:
A
摘要:
以大豆子叶节作为外植体，通过农杆菌介导法将含有BrCS基因的pCAMBIA3300的双子叶植物表达载体，导入到大豆品种黑农59和黑农53中，并对黑农59遗传转化的影响因素进行了优化，最终获得了抗性植株。经PCR检测和PCR-Southern杂交分析，初步证明了目的基因已整合到大豆的基因组中。
Abstract:
In this study,plant expression vector pCAMBIA3300-BrCS containing-BrCS-gene was constructed,and transgenic plants were obtained by Agrobacterium-mediated transformation of soybean(Heinong 59 and Heinong53)cotyledonary nodes.We regenerated plants and optimized the conditions of transformation to increase the efficiency of transformation.PCR and PCR-Southern detection was used to identify the integrated level of BrCS gene in transgenic plants;it confirmed the integration of BrCS genes into the genome of soybean.

参考文献/References:

[1]梁慧敏,夏阳,王太明.植物抗寒冻、抗旱、耐盐基因工程研究进展[J].草业学报,2003,12(3):1-7.（Liang H M,Xia Y,Wang T M.Progress on cold,drought and salt resistance gene engineering in plants[J].Acta Prataculturae Sinica,2003,12(3):1-7.）

[2]程继东,安玉麟,孙瑞芬,等.抗旱、耐盐基因类型及其机理的研究进展[J].华北农学报,2006,21(1):116-120.（Cheng J D,An Y L,Sun R F,et al.Progress on drought,salt gene types and their acting mechanisms[J].Acta Agriculturae Boreali-Sinica,2006,21(1):116-120.）

[3]姚荣江,杨劲松,刘广明.东北地区盐碱土特征及其农业生物治理[J].土壤,2006,38(3):256-262.（Yao R J,Yang J S,Liu G M.Charaeteristics and agro-biological management of saline-alkalized land in northeast China[J].Soils,2006,38(3):256-262.）

[4]Li Z,Zhao L,Sun,X,et al.Brassica rapa?subsp.pekinensis salt and low temperature response protein mRNA,GenBank:AY601678.1.

[5]刘文萍,卢淑雯,刘建新，等.2009.农杆菌介导的BnCS基因对黄瓜遗传转化研究[J].北方园艺,2009(1):20-22.(Liu W P,Lu S W,Liu Jian X,et al.Agrobacterium-mediated transformation of cucumber for BnCS?gene[J].Northern Horticulture,2009(1):20-22.）

[6]J.萨姆布鲁克,D.W.拉塞尔.分子克隆实验指南[M].北京:科学出版社,2008:27-138.(Joseph S,David R.Molecular cloning:A laboratory manual[M].Beijing:Science Press,2008:27-138.)

[7]唐晓飞.农杆菌介导hsf8基因转化大豆的研究[D].哈尔滨:东北农业大学,2008：23.(Tang X F.Studies on Agrobacterium-mediated transtormation of hsf8?into soybean[D].Northeast Agricultural University,2008:23.)

[8]李茂福,李睿,傅永福,等.农杆菌介导大豆遗传转化的影响因素[J].山地农业生物学报,2006,25(4):283-286.（Li M F,Li R,Fu Y F,et al.Influencing factors on the efficiency ofAgrobacterium-mediated soybean transformation[J].Journal of Mountain Agriculture and Biology,2006,25(4):283-286.）

[9]〖JP3〗杨荣仲,谭裕模,李松,等.大豆子叶节再生及农杆菌介导转化研究[J].西南农业学报,2003,16(4):33-38.（Yang R Z,Tan Y M,Li S,et al.Studies on regeneration of soybean cotyledon node and Agrobacterium-mediated genetic transformation[J].Southwest China Journal of Agricultural Sciences,2003,16(4):33-38.）

[10]李桂兰,乔亚科,杨少辉,等.农杆菌介导大豆子叶节遗传转化的研究[J].作物学报,2005,31(2):170-176.（Li G L,QiaoY K,Yang S H,et al.Study of the Agrobacterium-mediated transformation systems of soybean cotyledonary node[J].Acta Agronomica Sinica,2005,31(2):170-176.）

[11]姬月梅,陈受宜,李英慧,等.农杆菌介导大豆子叶节遗传转化体系的优化研究[J].大豆科学,2008,27(1):27-32.（Ji Y M,Chen S Y,Li Y H,et al.Optimization of genetic transformation system from soybean cotyledon mediated by Agrobacterium[J].Soybean Science,2008,27(1):27-32.）

[12]刘尚前,王罡,季静,等.农杆菌介导法转化大豆最佳条件的研究[J].吉林农业大学学报,2007,29(1):44-47.（Liu S Q,Wang G,Ji J,et al.Optimization of conditions for soybean genetic transformation mediated by Agrobacterium[J].Journal of Jilin Agricultural University,2007,29(1):44-47.）

[13]崔少彬.ODREB2B基因转化马铃薯及转BcBCP1基因马铃薯后代鉴定[D].哈尔滨:东北农业大学,2009:37.(Cui S B.The gene transformation of ODREB2B?gene in potato and identification of transgenic BcBCP1?gene of the progenies in potato[D].Northeast Agricultural University,2009:37.)

[14]Hayashi K，Hashimoto N，Daigen M,et al.Development of PCR-based SNP markers for rice blast resistance genes at the Pizlocus[J].Theoretical and Applied Genetics.2004,108:1212-1220.

[15]刘晋峰,邢达,沈行燕，等.电化学发光PCR技术检测转基因植物[J].生物化学与生物物理进展,2004,31(4):375-378.（Liu J F,Xing D,Shen X Y,et al.Detection of genetically modified plant by electrochemiluminescence PCR method[J].Progress in Biochemistry and Biophysics，2004,31(4):375-378.）


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备注/Memo

基金项目：国家转基因重大专项资助项目（2008ZX08004-002）；现代农业产业技术体系资助项目（nycytx-004）。

第一作者简介：张书利（1984-），男，硕士，研究方向为作物遗传育种。E-mail：qkpx1984@163.com。
通讯作者：刘丽君（1958-），女，研究员，从事大豆遗传育种研究。E-mail：nkyssbd@126.com。

更新日期/Last Update: 2014-08-15