ZHANG Qing-lin,ZHAO Yan,ZHANG Yan,et al.Sensitivity of Different Genotype Soybean Callus to Agrobacterium EHA105[J].Soybean Science,2011,30(04):566-568.[doi:10.11861/j.issn.1000-9841.2011.04.0566]
不同基因型大豆愈伤组织对农杆菌EHA105的敏感性研究
- Title:
- Sensitivity of Different Genotype Soybean Callus to Agrobacterium EHA105
- 文章编号:
- 1000-9841（2011）04-0566-03
- Keywords:
- Soybean; Callus; Genotype; GUS staining; Agrobacteriem tumefaciens
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 研究了8个基因型的大豆愈伤组织GUS瞬时表达的效果及其最佳染色条件，以考察不同基因型大豆愈伤组织对农杆菌菌株EHA105的敏感性。GUS染色结果表明：不同品种对农杆菌EHA105敏感程度差别较大，吉林47和东农42愈伤GUS染色效果较好；不同品种要求的最佳染色时间存在差异，对农杆菌EHA105较敏感的2个基因型吉林47和东农42，较适宜的GUS染色时间为9~10 h。
- Abstract:
- Soybean genotype has great impact on efficiency of genetic transformation.It is easy and convenient to evaluate the transgenic soybean by transient expression of GUS gene.This paper studied the sensitivity of eight genotypes to ?Agrobacterium EHA105 by GUS gene transient expression and staining times of GUS gene in soybean callus.The results showed that the sensitivity to Agrobacterium EHA105 varied with genotypes,the staining of Jilin47 and Dongnong42 were the best among the 8 soybean types indicated that soybean callus of Jilin47 and Dongnong42 were more sensitive to EHA105;the staining time was also varied with genotypes,appropriate GUS staining time was from 9 to 10 h.
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备注/Memo
基金项目：转基因生物新品种培育重大专项子课题（2008ZX08004-003）；国家自然科学基金面上资助项目（30971808）；吉林省科技发展计划重点资助项目（20080204）；长春市科技局国际科技合作资助项目（08GH10）；“211”三期建设资助项目。