HE Hai-yan,SHA Wei,ZHANG Yan-fu.Genetic Diversity Analysis of Soybean Germplasm in Heilongjiang Province by ISSR Markers[J].Soybean Science,2011,30(01):37-40.[doi:10.11861/j.issn.1000-9841.2011.01.0037]
黑龙江省大豆种质资源遗传多样性的ISSR分析
- Title:
- Genetic Diversity Analysis of Soybean Germplasm in Heilongjiang Province by ISSR Markers
- 文章编号:
- 1000-9841（2011）01-0037-04
- Keywords:
- Soybean; ISSR; Genetic diversity
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 采用ISSR分子标记技术对黑龙江省25个大豆品种进行遗传多样性分析。结果表明：从28对随机引物中筛选出9对多态性明显、条带清晰、反应稳定的引物；9对引物共扩增出65条带，其中58条为多态性条带，多态性比率为89.23%；Shannon多样性指数平均为0.4604，每个位点的有效等位基因数为1.5139；25个品种间的遗传相似性系数变幅范围为0.4462~0.8923，平均为0.6692；UPGMA聚类分析在GS = 0.698处，将25份大豆材料分成5大类。
- Abstract:
- The genetic diversities of 25 soybean varieties were studied by ISSR markers, 9 polymorphic primers were selected from 28 ISSR primers, 65 bands were amplified with 9 primers, they contained 58 polymorphic bands and their polymorphism percentage was 89.23%; Shannon’s index evaluation indicted that average Shannon’s index was 0.4604 and the numbers of effective alleles were 1.5139; the genetic similarity coefficient among the 25 varieties ranged from 0.4462 to 0.8923, and the average GS was 0.6692; cluster analysis with UPGMA method showed that the cultivars tested in this study could be divided into five groups at the level GS 0.698.
参考文献/References:
［1］ 〖ZK（#〗邱丽娟，曹永生，常汝镇，等．中国大豆(Glycine max)核心种质构建 Ⅰ.
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备注/Memo
基金项目：黑龙江省教育厅科研资助项目(10551331)。?