SHA Wei,HE Hai-yan,ZHANG Yan-fu.Gliadin Genetic Diversity in Soybean Varieties in Heilongjiang Province[J].Soybean Science,2010,29(06):934-937.[doi:10.11861/j.issn.1000-9841.2010.06.0934]
黑龙江省大豆品种醇溶蛋白的遗传多样性分析
- Title:
- Gliadin Genetic Diversity in Soybean Varieties in Heilongjiang Province
- 文章编号:
- 1000-9841（2010）06-0934-04
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 采用SDS-聚丙烯酰胺凝胶电泳技术（SDS-PAGE）对来自黑龙江省的25份大豆品种进行醇溶蛋白遗传多样性分析。结果供试材料共分离出19条谱带，多态性条带12条，多态性比率达63.16％，其中α区3条带，β区5条带，γ区5条带，ε区6条带。材料间的Nei-Li遗传相似性系数（GS）范围为0.4737~0.9474，平均值为0.7105；遗传距离（GD）范围为0.0541~0.7472，平均值为0.3654，表明供试材料之间具有较为丰富的醇溶蛋白遗传多样性。聚类分析结果表明，在GS值为0.79处可聚为4类，主成分分析进一步验证了此结果。由此可见，醇溶蛋白标记技术能很好地用来区分不同的大豆品种，为大豆品种鉴定和种质资源利用奠定基础。
- Abstract:
- The SDS polyacrylamide gel eleetrophoresis(SDS-PAGE) was used to detect the gliadin genetic diversity of 25 soybean varieties in Heilongjiang province, and the results were as follows: 1) A total of 19 bands were detected in 25 cultivars, the polymorphic bands were 12, amount to 63.16％, among α, β, γ and ε zones，there were 3, 5, 5 and 6 bands, respectively. The Nei’s genetic similarity coefficient of the tested cultivars ranged from 0.4737 to 0.9474, and the average GS was 0.7105;the genetic distances of the tested cultivars ranged from 0.0541 to 0.7472, the average GD was 0.3654, these results suggested that there was a rich genetic polymorphism of gliadin among the cultivars. 2) According to the cluster analysis, the tested cultivars could be classified into 4 groups at the level GS 0.79, and principal component analysis verified the results further. Thus, gliadin clusters markers could efficiently classify the soybean varieties, and laid the foundation for soybean variety identification and the utilize of germplasm resources.
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备注/Memo
基金项目：黑龙江省教育厅科研资助项目(10551331)。?