[1]张瑞朋,佟斌,傅连舜,等.氮素对不同来源大豆品种叶片保护酶的影响[J].大豆科学,2009,28(05):833-836.[doi:10.11861/j.issn.1000-9841.2009.05.0833]
　ZHANG Rui-peng,TONG Bin,FU Lian-shun,et al.Effects of Top-dressing Nitrogen on Protective Enzymes of Soybean Leaves from Different Regions[J].Soybean Science,2009,28(05):833-836.[doi:10.11861/j.issn.1000-9841.2009.05.0833]
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氮素对不同来源大豆品种叶片保护酶的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年05期 页码: 833-836 栏目: 出版日期: 2009-10-25
Title:
Effects of Top-dressing Nitrogen on Protective Enzymes of Soybean Leaves from Different Regions
文章编号:
1000-9841(2009)05-0833-04
作者:
张瑞朋1; 佟斌2; 傅连舜1; 杨德忠1; 谢甫绨3
1.铁岭市农业科学院，辽宁 铁岭112616；
2.辽宁职业学院，辽宁 铁岭 112001；
3.沈阳农业大学 农学院，辽宁 沈阳 110161
Author(s):
ZHANG Rui-peng1; TONG Bin2; FU Lian-shun1; YANG De-zhong1; XIE Fu-ti3
1.Tieling Academy of Agricultural Sciences，Tieling 112616，Liaoning；
2.Liaoning Vocational College，Tieling 112001，Liaoning；
3.Agronomy College of Shenyang Agricultural University，Shenyang 110161，Liaoning，China
关键词:
大豆; 氮; 超氧化物歧化酶; 过氧化物酶; 过氧化氢酶
Keywords:
Soybean; Nitrogen; SOD; POD; CAT
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.05.0833
文献标志码:
A
摘要:
采用12个不同来源的大豆品种为试材，并按来源分成3组，分别为俄亥俄当代品种、辽宁当代品种和辽宁老品种，采用苗期追施尿素（0，100，200 kg.hm-2）处理，研究了氮素对大豆叶片保护酶的影响。结果表明：当尿素施用量为100 kg.hm-2时，开花期俄亥俄当代品种与辽宁老品种叶片超氧化物歧化酶活性提高，辽宁当代品种与辽宁老品种结荚期叶片超氧化物歧化酶活性提高。随着施氮量的增加，辽宁当代品种开花期叶片超氧化物歧化酶活性有增加趋势；俄亥俄当代品种结荚期叶片超氧化物歧化酶活性也逐渐增强；不同大豆品种开花期叶片过氧化物酶与过氧化氢酶活性不断增加；和不施肥相比，100 kg.hm-2尿素施用处理使3组不同来源大豆品种鼓粒期叶片过氧化氢酶的活性得到提高。在生产上，采用100 kg.hm-2尿素追肥处理，可以提高大豆叶片保护酶活性，进而提高大豆的抗逆能力。
Abstract:
Nitrogen is very important for soybean growth.It is controversy whether additional nitrogen fertilizer needed or not in soybean production as soybean rhizobium can fix nitrogen.The experiment was carried out by using twelve soybean cultivars from different regions.And the cultivars were divided into three groups：current Ohio cultivar，current Liaoning cultivar and old Liaoning cultivar.Urea fertilizer (0，100，200 kg.ha-1) was top-dressed at soybean seedling stage.The activity of SOD，POD and CAT，three protective enzymes of soybean leaves at blooming，podding and grain filling were measured.Under 100 kg.ha-1nitrogen fertilizer treatment，the SOD activity of current Ohio cultivar and old Liaoning cultivar was increased at blooming stage，while SOD activity of current Liaoning cultivar and old Liaoning cultivar was increased at podding stage.The SOD activity showed increasing trend with the level of nitrogen fertilizer for current Liaoning cultivar at blooming stage，and current Ohio cultivar at podding stage.The POD and CAT activity of the three group soybean cultivar increased with the level of nitrogen fertilizer ascending at blooming stage.The CAT activity of three groups soybean cultivar improved in the treatment of 100 kg.ha-1urea fertilizer than that of control at grain filling stage.Results suggest that applying 100 kg.ha-1urea fertilizer by top-dressing can improve protective enzymes activity of leaves for different soybeans.

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备注/Memo

基金项目：辽宁省科技厅资助项目（2008201005）。

作者简介：张瑞朋（1979-），男，博士，农艺师，研究方向为大豆遗传育种与栽培技术。E-mail：zrp_tb@yahoo.com.cn。
通讯作者：谢甫绨，教授，博士生导师。E-mail:snsoybean@yahoo.com.cn。

更新日期/Last Update: 2014-09-15