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[1]汤复跃,张磊,陈培,等.大豆M型细胞质雄性不育恢复基因标记定位[J].大豆科学,2009,28(04):578-582.[doi:10.11861/j.issn.1000-9841.2009.04.0578]
　TANG Fuyue,ZHANG Lei,CHEN Pei,et al.Marker Location for Fertility Restorer Gene of MCytoplasmic Male Sterility in Soybean[J].Soybean Science,2009,28(04):578-582.[doi:10.11861/j.issn.1000-9841.2009.04.0578]

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大豆M型细胞质雄性不育恢复基因标记定位

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷期数: 2009年04期页码: 578-582 栏目: 出版日期: 2009-08-25

Title:: Marker Location for Fertility Restorer Gene of MCytoplasmic Male Sterility in Soybean

文章编号:: 1000-9841(2009)04-0578-05

作者:: 汤复跃¹; 张磊²; 陈培²; 陈渊¹; 梁江¹; 1广西玉米研究所，广西南宁532227；
2安徽省农业科学院作物研究所，安徽省农作物品质改良重点实验室，安徽合肥 230031

Author(s):: TANG Fuyue¹; ZHANG Lei²; CHEN Pei²; CHEN Yuan¹; LIANG Jiang¹; 1Guangxi Maize Research Institute，Nanning 532227，Guangxi；
2Crop Institute of Anhui Academy of Agricultural Sciences，Key Laboratory of Crop Quality Improvement of Anhui Province，Hefei 230031，Anhui，China)

关键词:: 大豆; 细胞质雄性不育; 微卫星标记

Keywords:: Soybean; Cytoplasmic male sterility (CMS); SSR

分类号:: S565.1

DOI:: 10.11861/j.issn.1000-9841.2009.04.0578

文献标志码:: A

摘要:: 鉴于与恢复基因紧密连锁的分子标记在分子辅助选择育种中的应用前景，采用SSR标记法定位大豆CMS恢复系WR016的恢复基因。根据（W931A×WR016）F₁、F₂的植株育性，分析表明大豆M型不育系统为单基因配子体不育。由于大豆M-CMS恢复系WR016的恢复基因定位在A1连锁群上，利用A1连锁群上的大豆SSR引物对不育系W931A和恢复系WR016构建的F₂分离群体进行分析，获得了与恢复基因连锁的三个标记Satt684、 Satt276和Satt545，遗传距离分别为29.5cM、10.7 cM和14.1 cM。虽然10.7 cM还是一个较远的距离，但为进一步精确定位恢复基因，并最终克隆恢复基因打下了基础。

Abstract:: In view of the application prospects of the molecular marker-assisted selection in breeding by the molecular markers closely linked with restorer gene，this experiment aims to locate restorer gene of the M-CMS WR016 with SSR markers.Fertilities of F₁，F₂generations of W931A(sterile line)×WR016(restorer line) showed that M-CMS belonged to single-gene gametophyte sterility.This experiment applies SSR molecular markers to locate the restorer gene of the restorer line WR016.Three SSR markers in A1 linkage group，Satt684，Satt276 and Satt545，genetically linked to the restorer gene，were selected by screening of SSR markers with F₂segregation population.The genetic distance between the markers and the restorer gene were 29.5，10.7 and 14.1 cM，respectively.Although 10.7 cM is still a large distance，it laid the foundation for further location of restorer gene.

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备注/Memo

基金项目：国家高技术研究发展计划（863计划）资助项目（2006AA100104）；国家科技支撑计划资助项目（2006BAD01A04）。

作者简介：汤复跃（1984-）男，硕士研究生，研究方向为植物分子遗传育种。 E-mail：tfy0130@163.com。

通讯作者：张磊，研究员。E-mail：leizh66@163.com。

更新日期/Last Update: 2014-09-21