[1]张永芳,黄昆,马中雨,等.大豆苗期固氮相关性状的QTL分析[J].大豆科学,2009,28(04):583-587,604.[doi:10.11861/j.issn.1000-9841.2009.04.0583]
　ZHANG Yong-fang,HUANG Kun,MA Zhong-yu,et al.Mapping of QTL for Controlling Nitrogen Fixation in Soybean Cultivars at Seedling Stage[J].Soybean Science,2009,28(04):583-587,604.[doi:10.11861/j.issn.1000-9841.2009.04.0583]
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大豆苗期固氮相关性状的QTL分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年04期 页码: 583-587,604 栏目: 出版日期: 2009-08-25
Title:
Mapping of QTL for Controlling Nitrogen Fixation in Soybean Cultivars at Seedling Stage
文章编号:
1000-9841(2009)04-0583-05
作者:
张永芳1; 3; 黄昆2; 马中雨4; 范海1; 李俊4; 常汝镇2; 邱丽娟2
1山东师范大学生命科学学院，山东 济南 250014；
2中国农业科学院作物科学研究所 国家农作物基因资源与遗传改良重大科学工程 农业部作物种质资源与生物技术重点开放实验室，北京100081；
3山西大同大学，山西 大同037009；
4中国农业科学院土壤肥料研究所，北京100081
Author(s):
ZHANG Yong-fang1; 3; HUANG Kun2; MA Zhong-yu4; FAN Hai 1; LI Jun4; CHANG Ru-zhen2; QIU Li-juan2
1Life Science College of Shandong Normal University，Jinan 250014，Shandong；
2The National Key Facility for Crop Gene Resource and Genetic Improvement(NFCRI)/Key Lab of Germplasm & Biotechnology(MOA)/Institute of Crop Science，Chinese Academy of Agricultural Sciences；Beijing 100081；
3.Shanxi Datong University，Datong 037009，Shanxi；
?4.Institute of Agricultural resources and Agricultural Regional Planning，Chinese Academy of Agricultural Sciences，Beijing 100081，China
关键词:
大豆; 生物固氮; QTL
Keywords:
Key words：Soybean; Nitrogen fixation; Quantitative trait loci（QTL）
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.04.0583
文献标志码:
A
摘要:
大豆与根瘤菌共生固氮是大豆生长发育所需氮素的主要来源。由于根瘤菌与大豆两者基因型的不同，接种根瘤菌后大豆固氮能力也不同。以合丰25×固新野生大豆杂交组合的重组自交系(RIL)群体F11的104个株系为材料，在严格控菌条件下，用固氮菌株2178进行结瘤匹配鉴定，测定RIL群体及其亲本的固氮酶活性、结瘤数目、侧根数目、根瘤鲜重、茎干重5个指标，对所得数据进行正态分布检验，结合SSR分子数据利用复合区间作图法对其QTL定位分析。结果表明：RIL群体各性状均表现超亲分离，均值介于双亲之间，其偏度和峰度均较小，符合正态分布。这表明所考察性状均为数量性状遗传。应用复合区间作图法进行固氮性状的QTL定位，在A1、L、O、D1b、D2、C2、I连锁群，检测控制固氮的QTL有8个，解释表型变异的7.65%~15.05%，这些QTL及分子标记位点可用于大豆固氮性状的分子标记选择。
Abstract:
Nitrogen demand of soybean can be supplied via biological nitrogen fixation.Because genotype of soybean and soybean rhizobium is different，the symbiotic ability is significantly different among soybeans after inoculation.In this study，the F11?populations derived from G.max Hefeng 25×G.soja Guxin were inoculated with B.japonicum strain 2178，and five indexes of RILs population and parents，including activity of nitrogen fixation，No.of nodulation，No.of lateral root，weight of nodulation and weight of stem，were identified at seedling stage.QTL mapping was performed by characteristics of nitrogen fixation trait combined with the composite interval mapping.All the nitrogen fixation traits at seedling stage showed a normal distribution in RIL population，which were suitable to QTL mapping analysis.Eight QTLs on chromosomes A1，L，D1b，D2，C2，I and O associated with nodulation traits at seedling stage were detected，which explained the range of the observed phenotypic variance from 7.65% to 15.05%.These markers can be used in molecular marker selection of soybean nitrogen fixation traits.

参考文献/References:

[1]沈世华，荆玉祥.中国生物固氮研究现状和展望[J].科学通报，2003，48(6)：535-540.（Shen S H，Jin Y X.Research and prospect of Chinese nitrogen fixation[J].Chinese Science Bulletin，2003，48(6)：535-540.）

[2]谭娟.接种俄罗斯大豆根瘤菌对大豆生长和产量的影响[J].作物杂志，2007，4：36-37.（Tan J.Influence on growth and yield of soybeans after Russia’s Rhizobia inoculation[J].Crops，2007，4：36-37.）
[3]王卫卫，胡正海.几种生态因素对西北干旱地区豆科植物结瘤固氮的影响[J].西北植物学报，2003，23(7)：1163-1168.（Wang W W，Hu Z H.Characteristics related to symbiotic nitrogen fixation of legumes in northwest arid zone of China[J].Acta Botanica Boreali-Occidentalia Sinica，2003，23(7)：1163-1168.）
[4]Nutman P S.Varietal differences in the nodulation of subterranean clover[J].Australian Journal of Agricultural Research，1967，18(3)：381-425.
[5]Hungria M，Stacey G.Molecular signal exchange between host plant and rhizobia：basic aspects and potential application in agriculture[J].Soil Biology and Biochemistry，1997，29(5-6)：819-830.
[6]谢荣，何志水，刘芳华，等.应用cDNA-AFLP技术鉴定紫花苜蓿(Medicago sativa)根瘤发育相关基因[J].科学通报，2006，51(15)：1794-1801.（Xie R，He Z S，liu F H，et al.Identification of gene in Medicago sativa during root nodule formation by cDNA-AFLP technique[J].Chinese Science Bulletin，2006，51(15)：1794-1801.）
[7]Puji L，Kyujung V，Moon Y，et al.Differentially expressed genes related to symbiotic association in a supernodulation soybean mutant and its wild types[J].Molecular Plant Pathology，2006，7(4)：235-247.
[8]Stougard J.Regulators and regulation of legume root nodule development[J].Plant Physiology，2000，124(2)：531-540.
[9]Colebatch G，Kloska S，Trevaskis B，et al.Novel aspects of symbiotic nitrogen fixation uncovered by transcript profiling with cDNA arrays[J].Molecular Plant-Microbe Interactions，2002，15：411-420.
[10]Kaneko T，Nakamura Y，Sato S，et al.Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110[J].DNA Research，2002，9：189-197.
[11]Shoemaker R C，Schlueter J A，Cregan P，et al.The status of soybean genomics and its role in the development of soybean biotechnologies[J].AgBioForum，2003，（1&2）：4-7.
[12]Wiel C，Scheres B，Franssen H，et al.The early nodulin transcript ENOD2 is located in the nodule parenchyma (inner cortex) of pea and soybean root nodules[J].The EMBO Journal，1990，9(1)：1-7.
[13]Scheres B，Engelen F，Knaap E，et al.Sequential induction of nodulin gene expression in the developing pea nodule al[J].Plant Cell，1990，2(8)：687-700.
[14]Pichon M，Journet E P，Dedieu A，et al.Rhizobium meliloti elicits transient expression of the early nodulin gene ENOD12 in the differentiating root epidermis of transgenic alfalfa[J].Plant Cell，1992，4(10)：1199-1211.
[15]Charon C，Johansson C，Kondorosi E，et al.Enod40 induces dedifferentiation and division of root cortical cells in legumes[J].Plant Biology，1997，94：8901-8906.
[16]Carroll B J，McNeil D L，Gresshoff P M.A super nodulating and nitrate-tolerant symbiotic (nts) soybean mutant[J].Plant Physiology，1985，78：34-40.
[17]Radutoiu S，Madsen L H，Madsen E B，et al.Plant recognition of symbiotic bacteria requires two LysM receptor-like kinases[J].Nature，2003，425：585-592.
[18]Madsen E B，Madsen L H，Radutoiu S，et al.A receptor kinase gene of the LysM type is involved in legume perception of rhizobial signals[J].Nature，2003，425：637-640.
[19]Limpens E，Franken C，Smit P，et al.LysM domain receptor kinases regulating rhizobial Nod factor-induced infection[J].Science，2003，302：630-633.
[20]Stracke S，Kistner C，Yoshida S，et al.A plant receptor-like kinase required for both bacterial and fungal symbiosis[J].Nature，2002，417：959-962.
[21]Endre G，Kereszt A，Kevei Z，et al.A receptor kinase regulating symbiotic nodule development[J].Nature，2002，417：962-966.
[22]Krusell L，Madsen L H，Sato S，et al.Shoot control of root development and nodulation is mediated by a receptor-like kinase[J].Nature，2002，420：422-425.
[23]Nishimura R，Hayashi M，Wu G J，et al.HAR1 mediates systemic regulation of symbiotic organ development[J].Nature，2002，420：426-429.
[24]Searle I R，Men A E，Laniya T S，et al.Long-distance signaling in nodulation directed by a CLAVATA1-like receptor kinase[J].Science，2003，299：109-112.
[25]Kim M Y，Van K，Lestari P，et al.SNP identification and SNAP marker development for a GmNARK gene controlling supernodulation in soybean[J].Theoretical and Applied Genetics，2005，110：1003-101.
[26]Kilen C，Palmer R G.Qualitative genetics and cytogenetics[J].Agronomy，1987，16：135-209.
[27]Landau-Ellis D，Angermüller S，Shoemaker R，et al.The Genetic locus controlling supernodulation in soybean (Glycine max L.) co-segregates tightly with a cloned molecular marker[J].Molecular and General Genetics，1991，228(1-2)：21-226.
[28]Nicolás M F，Hungria M，Arias C A A.Identification of quantitative trait loci controlling nodulation and shoot mass in progenies from two Brazilian soybean cultivars[J].Field Crops Research，2006，95(2-3)：355-366.
[29]Dandan Li，Pfeiffer T W，Cornelius P L.Soybean QTL for yield and yield components associated with Glycine soja alleles[J].Crop Science，2008，48：571-581.
[30]蒙忻，刘学义，方宣钧.利用大豆分子连锁图定位大豆孢囊线虫4号生理小种抗性QTL[J].分子植物育种，2003，1(1)：6-21.（Meng X，Liu X Y，Fang X J.QTL Mapping genes conferring resistance to race 4 of SCN[J].Molecular Plant Breeding，2003，1(1)：6-21.）

[31]Panthee D R，Kwanyuen P，Sams C E，et al.Quantitative trait loci for βconglycinin (7S) and glycinin(11S) fractions of soybean storage protein[J].Journal of the American Oil Chemists’ Society，2004，81(11)：1005-1012.
[32]Teuku T，Satosh W，Naoki Y，et al.Analysis of quantitative trait loci for protein and lipid contents in soybean seeds using recombinant inbred lines[J].Breeding Science，2003，53(2)：133-140.
[33]葛诚，徐玲攻，樊慧，等.快生型大豆根瘤菌的抗原分析[J].大豆科学，1984，3 (3)：237-242.（ Ge C，Xu L M，Fan H.Analysis of antigens for fast growing R.japonicum[J].Soybean Science，1994，13(4)：331-335.）
[34]葛诚，李俊，樊蕙等.大豆三类共生体的生态学研究[J].大豆科学，1994，13(4)：331-335.（Ge C，Li J，Fan H.Studies on ecology of the three groups of soybean rhizobiua[J].Soybean Science，1994，13(4)：331-335.）
[35]樊蕙，徐玲攻，葛诚等.大豆根瘤菌优良菌株与春大豆品种的亲和性研究[J].大豆科学，1992，11(2)：139-145.（Ge C，Xu L M，Fan H.Studies on compatibility between superior indigenous soybean Rhizobial strains and soybean cultivars[J].Soybean Science，1992，11(2)：139-145.）

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备注/Memo

基金项目：国家高技术研究发展计划（863计划）资助项目（2006AA100104）；国家科技支撑资助项目(2006BAD13B05)。

作者简介：张永芳(1982-），女，硕士研究生，研究方向为植物生理及分子生物学。黄昆为共同第一作者。
通讯作者：邱丽娟，博士，研究员。E-mail:qiu_lijuan@263.net。

更新日期/Last Update: 2014-09-21